breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 2,857 G→A V19V (GTG→GTA thrB → homoserine kinase
RA 13,942 G→A V594I (GTT→ATT)  dnaK → chaperone protein DnaK
RA 14,424 G→A G86D (GGC→GAC)  dnaJ → chaperone protein DnaJ
RA 23,088 G→A G233D (GGC→GAC)  ileS → isoleucine‑‑tRNA ligase
RA 26,614 G→A G113D (GGC→GAC)  ispH → 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase
RA 27,781 G→A E163E (GAG→GAA rihC → ribonucleoside hydrolase RihC
RA 35,476 G→A G229G (GGC→GGT caiD ← crotonobetainyl‑CoA hydratase
RA 51,290 C→T G106D (GGC→GAC)  apaG ← DUF525 domain‑containing protein ApaG
RA 93,591 C→T G142G (GGC→GGT murE → UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase
RA 109,960 G→A R561H (CGT→CAT)  secA → protein translocation ATPase
RA 177,404 A→T L74Q (CTG→CAG)  yadS ← PF03458 family protein YadS
RA 186,539 C→T T704T (ACG→ACA glnD ← protein‑PII uridylyltransferase/uridylyl‑removing enzyme
RA 202,615 C→T S19L (TCA→TTA)  lpxA → acyl‑[acyl‑carrier‑protein]‑‑UDP‑N‑ acetylglucosamine O‑acyltransferase
RA 246,747 G→T S12S (TCG→TCT yafL → NlpC/P60 family protein YafL
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 280,709 C→T W9* (TGG→TGA yagB ← orphan antitoxin YagB
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 294,093 G→A P236L (CCT→CTT)  yagM ← protein YagM
RA 313,543 G→A intergenic (‑301/‑1748) ykgR ← / → insE1 putative membrane protein YkgR/IS3 element protein InsE
JC JC 332,367 IS2 (–) +5 bp [pdeL] [pdeL]
RA 339,935 C→T A56V (GCC→GTC)  yahH → putative uncharacterized protein YahH
RA 339,958 G→A D64N (GAC→AAC)  yahH → putative uncharacterized protein YahH
RA 364,850 G→A Q486* (CAG→TAG)  lacZ ← beta‑galactosidase
RA 368,583 C→T intergenic (‑163/‑28) mhpR ← / → mhpA DNA‑binding transcriptional activator MhpR/3‑(3‑hydroxyphenyl)propanoate hydroxylase
RA 371,176 G→A E300E (GAG→GAA mhpB → 3‑carboxyethylcatechol 2,3‑dioxygenase
RA 371,683 C→T P149S (CCG→TCG)  mhpC → 2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase
RA 374,464 C→T H199H (CAC→CAT mhpE → 4‑hydroxy‑2‑oxovalerate aldolase
RA 378,493 C→T G360D (GGC→GAC)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA 385,763 C→T P178S (CCG→TCG)  tauA → taurine ABC transporter periplasmic binding protein
RA 395,831 G→A R153C (CGT→TGT)  ampH ← peptidoglycan DD‑carboxypeptidase/peptidoglycan DD‑endopeptidase
RA 397,530 G→A V298I (GTA→ATA)  sbmA → peptide antibiotic/peptide nucleic acid transporter
RA 401,991 C→T A82V (GCC→GTC)  phoA → alkaline phosphatase
RA 402,739 G→A L331L (TTG→TTA phoA → alkaline phosphatase
RA 403,368 C→T Q30* (CAA→TAA)  psiF → phosphate starvation‑inducible protein PsiF
RA 413,051 C→T G901G (GGG→GGA sbcC ← ATP‑dependent structure‑specific DNA nuclease ‑ SbcC subunit
RA 428,681 C→T L345L (CTC→CTT secD → Sec translocon accessory complex subunit SecD
RA 431,286 G→A A243V (GCT→GTT)  tsx ← nucleoside‑specific channel‑forming protein Tsx
RA 431,591 G→A R141R (CGC→CGT tsx ← nucleoside‑specific channel‑forming protein Tsx
RA 435,874 G→A A81T (GCC→ACC)  thiL → thiamine monophosphate kinase
RA 437,421 G→A L239F (CTC→TTC)  yajO ← 1‑deoxyxylulose‑5‑phosphate synthase YajO
RA 437,436 C→T E234K (GAG→AAG)  yajO ← 1‑deoxyxylulose‑5‑phosphate synthase YajO
RA 439,909 C→T G90E (GGA→GAA)  dxs ← 1‑deoxy‑D‑xylulose‑5‑phosphate synthase
RA 441,092 G→A P4S (CCG→TCG)  ispA ← geranyl diphosphate/farnesyl diphosphate synthase
RA 441,907 G→A S120N (AGC→AAC)  thiI → tRNA uridine 4‑sulfurtransferase
RA 447,020 G→A A229V (GCT→GTT)  cyoE ← heme O synthase
RA 448,014 G→A S11S (TCC→TCT cyoD ← cytochrome bo3 subunit 4
RA 451,013 G→A P200S (CCC→TCC)  cyoA ← cytochrome bo3 subunit 2
RA 455,171 C→T R13R (CGC→CGT tig → trigger factor
RA 459,694 G→A E269E (GAG→GAA lon → Lon protease
RA 460,576 G→A K563K (AAG→AAA lon → Lon protease
RA 462,097 C→T S61S (AGC→AGT ppiD → periplasmic folding chaperone
RA 464,112 G→A G59D (GGC→GAC)  ybaV → helix‑hairpin‑helix 3 family protein
RA 466,723 G→A A197V (GCA→GTA)  ybaE ← uncharacterized protein YbaE
RA 467,244 G→A T23T (ACC→ACT ybaE ← uncharacterized protein YbaE
RA 474,558 C→T A202T (GCT→ACT)  tesB ← acyl‑CoA thioesterase II
RA 478,694 G→A intergenic (‑77/+87) pdeB ← / ← ylaC c‑di‑GMP phosphodiesterase PdeB/putative inner membrane protein
RA 480,295 2 bp→AA coding (13‑14/219 nt) hha ← hemolysin expression‑modulating protein Hha
RA 498,094 G→A G14S (GGT→AGT)  hemH → ferrochelatase
RA 501,402 C→T S426S (AGC→AGT gsk → inosine/guanosine kinase
RA 509,329 G→A A684V (GCC→GTC)  copA ← Cu(+) exporting P‑type ATPase
RA 509,517 C→T E621E (GAG→GAA copA ← Cu(+) exporting P‑type ATPase
RA 510,530 G→A L284F (CTT→TTT)  copA ← Cu(+) exporting P‑type ATPase
RA 511,514 G→A intergenic (‑135/‑127) copA ← / → glsA Cu(+) exporting P‑type ATPase/glutaminase 1
RA 513,659 G→A A362T (GCG→ACG)  ybaT → putative transporter YbaT
RA 516,955 C→T P125S (CCT→TCT)  fetB → putative iron ABC exporter membrane subunit FetB
RA 527,162 C→T T1301M (ACG→ATG)  rhsD → protein RhsD
RA 537,976 C→T A115V (GCC→GTC)  ybbW → putative allantoin transporter
RA 544,586 C→T R236H (CGT→CAT)  allC ← allantoate amidohydrolase
RA 553,298 C→T V215I (GTA→ATA)  lpxH ← UDP‑2,3‑diacylglucosamine diphosphatase
RA 554,298 C→T G47D (GGC→GAC)  ppiB ← peptidyl‑prolyl cis‑trans isomerase B
RA 555,459 C→T Y283Y (TAC→TAT cysS → cysteine‑‑tRNA ligase
RA 576,410 G→A pseudogene (416/1040 nt) nmpC ← putative outer membrane porin NmpC
RA 579,076 G→A intergenic (‑183/+108) borD ← / ← ybcV prophage lipoprotein BorD/DUF1398 domain‑containing protein YbcV
RA 612,410 G→A P29S (CCT→TCT)  fepA ← ferric enterobactin outer membrane transporter
RA 622,820 G→A G174D (GGC→GAC)  entS → enterobactin exporter EntS
RA 631,537 G→A W548* (TGG→TGA cstA → pyruvate transporter CstA
RA 633,227 C→T G84D (GGT→GAT)  hcxA ← hydroxycarboxylate dehydrogenase A
RA 690,948 G→A V279V (GTC→GTT ybeX ← CorC‑HlyC family protein YbeX
RA 700,038 G→A H97Y (CAT→TAT)  umpH ← UMP phosphatase
RA 700,628 C→T G323S (GGT→AGT)  nagC ← DNA‑binding transcriptional dual regulator NagC
RA 809,217 2 bp→AG coding (40‑41/1290 nt) bioA ← adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
RA 869,418 G→A R3K (AGA→AAA)  gsiB → glutathione ABC transporter periplasmic binding protein
RA 871,045 G→A V27I (GTC→ATC)  gsiC → glutathione ABC transporter membrane subunit GsiC
RA 873,115 G→A R46H (CGC→CAC)  pdeI → putative c‑di‑GMP phosphodiesterase PdeI
RA 884,344 G→A G224G (GGG→GGA mdfA → multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 897,642 G→A V187I (GTC→ATC)  potI → putrescine ABC transporter membrane subunit PotI
RA 909,012 G→A L95L (CTG→TTG)  ltaE ← low‑specificity L‑threonine aldolase
RA 914,713 C→T A49T (GCG→ACG)  lysO ← L‑lysine/thialysine exporter
RA 924,350 C→T P363S (CCG→TCG)  clpA → ATP‑dependent Clp protease ATP‑binding subunit ClpA
RA 968,837 G→A D158N (GAT→AAT)  lpxK → tetraacyldisaccharide 4'‑kinase
RA 987,152 G→T intergenic (‑170/+433) ompF ← / ← asnS outer membrane porin F/asparagine‑‑tRNA ligase
RA 987,159 T→A intergenic (‑177/+426) ompF ← / ← asnS outer membrane porin F/asparagine‑‑tRNA ligase
RA 1,030,625 C→T G15D (GGC→GAC)  tusE ← sulfur transfer protein TusE
RA 1,040,126 G→T L277L (CTG→CTT appB → cytochrome bd‑II subunit 2
RA 1,070,014 A→G L114P (CTG→CCG)  rutF ← FMN reductase RutF
RA 1,106,559 G→A A69T (GCA→ACA)  clsC → cardiolipin synthase C
RA 1,109,816 G→A G161D (GGT→GAT)  opgG → osmoregulated periplasmic glucans biosynthesis protein G
RA 1,111,727 2 bp→TT coding (865‑866/2544 nt) opgH → osmoregulated periplasmic glucans biosynthesis protein H
RA 1,111,867 G→A W335* (TGG→TGA opgH → osmoregulated periplasmic glucans biosynthesis protein H
RA 1,113,805 G→A *76* (TGA→TAA)  yceK → DUF1375 domain‑containing lipoprotein YceK
RA 1,115,668 G→A R305R (CGC→CGT lpxL ← lauroyl acyltransferase
RA 1,125,322 G→A S2F (TCC→TTC)  mdtH ← multidrug efflux pump MdtH
RA 1,127,608 G→A G268D (GGC→GAC)  yceM → putative oxidoreductase YceM
RA 1,132,149 G→A G99D (GGC→GAC)  flgD → flagellar biosynthesis, initiation of hook assembly
RA 1,132,865 G→A G98R (GGA→AGA)  flgE → flagellar hook protein FlgE
RA 1,135,235 G→A Q169Q (CAG→CAA flgG → flagellar basal‑body rod protein FlgG
RA 1,136,053 G→A V164M (GTG→ATG)  flgH → flagellar L‑ring protein
RA 1,140,714 G→A A228T (GCG→ACG)  flgL → flagellar hook‑filament junction protein 2
RA 1,150,616 C→T L297L (CTG→TTG)  fabD → [acyl‑carrier‑protein] S‑malonyltransferase
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,183,662 G→A Y261Y (TAC→TAT potB ← spermidine preferential ABC transporter membrane subunit PotB
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,276,230 C→T P463P (CCG→CCA narX ← sensor histidine kinase NarX
RA 1,286,364 C→T G172G (GGC→GGT narI → nitrate reductase A subunit gamma
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
MC JC 1,302,147 Δ5 bp coding (966‑970/1632 nt) oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,310,770 A→G H32H (CAT→CAC yciI ← protein YciI
RA 1,315,384 G→A Y129Y (TAC→TAT yciF ← DUF892 domain‑containing protein YciF
RA 1,317,129 G→A Q32* (CAG→TAG)  trpA ← tryptophan synthase subunit alpha
RA 1,320,907 C→T D160N (GAT→AAT)  trpD ← anthranilate synthase subunit TrpD
RA 1,322,325 G→A L208F (CTC→TTC)  trpE ← anthranilate synthase subunit TrpE
RA 1,327,979 C→T D127N (GAT→AAT)  btuR ← cobinamide/cobalamin adenosyltransferase
RA 1,329,672 C→T A114V (GCC→GTC)  sohB → S49 peptidase family protein
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,340,381 G→A V47I (GTA→ATA)  lapA → lipopolysaccharide assembly protein A
RA 1,340,390 G→A A50T (GCT→ACT)  lapA → lipopolysaccharide assembly protein A
RA 1,341,035 G→A A160T (GCA→ACA)  lapB → lipopolysaccharide assembly protein B
RA 1,351,182 C→T intergenic (‑143/+225) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD
RA 1,351,623 G→A Y46Y (TAC→TAT ycjD ← DUF559 domain‑containing protein YcjD
RA 1,356,258 G→A P285S (CCG→TCG)  sapA ← putative periplasmic binding protein SapA
RA 1,356,932 G→A T60I (ACC→ATC)  sapA ← putative periplasmic binding protein SapA
RA 1,357,202 C→T intergenic (‑92/+221) sapA ← / ← ymjA putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
RA 1,358,331 C→T G286D (GGC→GAC)  puuP ← putrescine:H(+) symporter PuuP
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,362,490 G→A intergenic (+22/‑253) puuR → / → puuC DNA‑binding transcriptional repressor PuuR/gamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase
RA 1,363,627 C→T F295F (TTC→TTT puuC → gamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase
RA 1,366,277 G→A G243D (GGC→GAC)  puuE → 4‑aminobutyrate aminotransferase PuuE
RA 1,366,433 G→A G295D (GGC→GAC)  puuE → 4‑aminobutyrate aminotransferase PuuE
RA 1,369,455 G→A K21K (AAG→AAA pspD → phage shock protein D
RA 1,370,821 C→T Y202Y (TAC→TAT ycjM → glucosylglycerate phosphorylase
RA 1,372,825 G→A G306E (GGG→GAG)  ycjN → putative ABC transporter periplasmic binding protein YcjN
RA 1,374,201 G→A G38S (GGC→AGC)  ycjP → putative ABC transporter membrane subunit YcjP
RA 1,376,784 G→A A251T (GCG→ACG)  ycjR → 3‑dehydro‑D‑guloside 4‑epimerase
RA 1,380,629 G→A G161D (GGC→GAC)  ycjU → beta‑phosphoglucomutase
RA 1,383,388 G→A P192S (CCG→TCG)  ycjW ← DNA‑binding transcriptional repressor YcjW
RA 1,399,042 G→A S162L (TCG→TTG)  fnr ← DNA‑binding transcriptional dual regulator FNR
RA 1,400,978 C→T G266S (GGT→AGT)  abgT ← p‑aminobenzoyl glutamate:H(+) symporter
RA 1,410,100 G→A R197Q (CGA→CAA)  dbpA → ATP‑dependent RNA helicase DbpA
RA 1,416,797 G→A P197L (CCT→CTT)  recE ← exonuclease VIII, ds DNA exonuclease, 5' ‑‑> 3' specific
MC JC 1,428,795 Δ115,337 bp IS5‑mediated lomRnarU 104 genes
RA 1,554,719 C→T Q317Q (CAG→CAA maeA ← malate dehydrogenase (oxaloacetate‑decarboxylating)
RA 1,603,321 C→T L101L (CTC→CTT lsrC → Autoinducer‑2 ABC transporter membrane subunit LsrC
RA 1,604,223 C→T G59G (GGC→GGT lsrD → Autoinducer‑2 ABC transporter membrane subunit LsrD
RA 1,604,840 C→T S265F (TCC→TTC)  lsrD → Autoinducer‑2 ABC transporter membrane subunit LsrD
RA 1,605,533 C→T S161S (AGC→AGT lsrB → Autoinducer‑2 ABC transporter periplasmic binding protein
RA 1,606,464 C→T A122V (GCC→GTC)  lsrF → 3‑hydroxy‑2,4‑pentadione 5‑phosphate thiolase
RA 1,608,089 C→T A248A (GCC→GCT tam → trans‑aconitate 2‑methyltransferase
RA 1,628,147 G→A D211N (GAT→AAT)  ydfG → 3‑hydroxy acid dehydrogenase YdfG
JC JC 1,633,913 IS5 (–) +4 bp coding (292‑295/591 nt) pinQ → putative recombinase PinQ
RA 1,643,554 C→T E251K (GAA→AAA)  ydfU ← protein YdfU
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,645,875 C→T intergenic (‑3/‑22) relB ← / → ydfV antitoxin/DNA‑binding transcriptional repressor RelB/protein YdfV
RA 1,648,720 C→T A19V (GCC→GTC)  ydfB → uncharacterized protein YdfB
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,677,341 C→T G188D (GGC→GAC)  pntA ← pyridine nucleotide transhydrogenase subunit alpha
RA 1,712,661 A→T intergenic (+503/‑108) nth → / → dtpA endonuclease III/dipeptide/tripeptide:H(+) symporter DtpA
RA 1,721,136 C→T L38F (CTT→TTT)  ydhI → DUF1656 domain‑containing protein YdhI
RA 1,743,057 G→A D62D (GAC→GAT ribC ← riboflavin synthase
RA 1,776,715 A→T S377C (AGT→TGT)  ydiF → putative acetate‑CoA transferase YdiF
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
JC JC 1,907,451 IS2 (+) +5 bp coding (137‑141/144 nt) yobF ← DUF2527 domain‑containing protein YobF
RA 1,919,728 C→T T740I (ACT→ATT)  letB → lipophilic envelope spanning tunnel
RA 1,978,245 A→C intergenic (‑48/+273) flhD ← / ← insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
RA 1,986,333 A→G intergenic (‑205/‑592) araF ← / → ftnB arabinose ABC transporter periplasmic binding protein/putative ferritin‑like protein
RA 1,987,499 C→A intergenic (+71/+8) ftnB → / ← yecJ putative ferritin‑like protein/DUF2766 domain‑containing protein YecJ
RA 1,991,071 C→T M183I (ATG→ATA yecA ← putative iron‑binding protein YecA
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
JC JC 2,174,817 IS1 (+) +9 bp coding (225‑233/453 nt) gatA ← galactitol‑specific PTS enzyme IIA component
RA 2,188,087 T→C T110A (ACG→GCG)  yehA ← putative fimbrial adhesin YehA
RA 2,195,035 G→A A246T (GCG→ACG)  metG → methionine‑‑tRNA ligase
RA 2,257,606 C→T Q254Q (CAG→CAA psuG ← pseudouridine‑5'‑phosphate glycosidase
RA 2,270,933 C→T P70S (CCG→TCG)  pdeN → putative c‑di‑GMP phosphodiesterase PdeN
RA 2,286,554 C→T pseudogene (2361/2525 nt) yejO ← adhesin‑like autotransporter YejO
RA 2,384,808 C→T K305K (AAG→AAA yfbK ← IPR002035/DUF3520 domain‑containing protein YfbK
JC JC 2,406,778 IS5 (+) +4 bp intergenic (‑137/‑780) lrhA ← / → alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
RA 2,410,419 G→A L312L (CTG→TTG)  yfbS ← putative transporter YfbS
RA 2,415,485 G→A G247S (GGC→AGC)  pta → phosphate acetyltransferase
RA 2,484,827 C→T L152F (CTT→TTT)  evgS → sensor histidine kinase EvgS
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,600,716 G→A R80H (CGT→CAT)  bcp → thiol peroxidase
RA 2,602,942 G→A G375G (GGG→GGA hyfB → hydrogenase 4 component B
RA 2,603,828 G→A A671T (GCC→ACC)  hyfB → hydrogenase 4 component B
RA 2,609,438 G→A E318K (GAA→AAA)  hyfG → hydrogenase 4 catalytic subunit HyfG
RA 2,622,358 G→A G42D (GGC→GAC)  purN → phosphoribosylglycinamide formyltransferase 1
RA 2,646,753 G→A S191S (TCC→TCT pbpC ← peptidoglycan glycosyltransferase PbpC
RA 2,654,454 G→A T160T (ACC→ACT sseB ← protein SseB
RA 2,678,218 G→A G48G (GGC→GGT yphE ← putative ABC transporter ATP‑binding protein YphE
RA 2,692,949 G→A G865G (GGC→GGT purL ← phosphoribosylformylglycinamide synthetase
RA 2,698,650 G→A intergenic (‑100/‑109) pgpC ← / → yfhH phosphatidylglycerophosphatase C/putative DNA‑binding transcriptional regulator YfhH
RA 2,713,679 C→T H262Y (CAT→TAT)  srmB → ATP‑dependent RNA helicase SrmB
RA 2,718,545 G→A intergenic (‑17/‑190) yfiF ← / → trxC putative methyltransferase YfiF/reduced thioredoxin 2
RA 2,722,605 G→A E885K (GAA→AAA)  patZ → peptidyl‑lysine N‑acetyltransferase
RA 2,725,233 C→T G172R (GGA→AGA)  kgtP ← alpha‑ketoglutarate:H(+) symporter
RA 2,736,417 C→T A91V (GCC→GTC)  bamD → outer membrane protein assembly factor BamD
RA 2,746,932 G→A D288D (GAC→GAT ffh ← signal recognition particle protein component
RA 2,763,207 G→A L112F (CTC→TTC)  abpB ← putative helicase YfjK
RA 2,766,540 G→A R208H (CGC→CAC)  rnlA → RNase LS, toxin of the RnlAB toxin‑antitoxin system
RA 2,767,481 G→A intergenic (+126/‑229) rnlB → / → yfjP antitoxin RnlB/putative GTP‑binding protein YfjP
RA 2,776,896 G→A R7H (CGC→CAC)  ypjJ → DUF987 domain‑containing protein YpjJ
RA 2,777,980 Δ2 bp intergenic (+198/+165) ypjF → / ← ypjA toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter YpjA
RA 2,777,984 Δ1 bp intergenic (+202/+162) ypjF → / ← ypjA toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter YpjA
RA 2,796,259 G→A A196T (GCG→ACG)  glaR → DNA‑binding transcriptional repressor GlaR
RA 2,867,430 G→A A41V (GCC→GTC)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,875,975 G→A L119L (CTG→TTG)  cysD ← sulfate adenylyltransferase subunit 2
RA 2,880,433 G→A R206* (CGA→TGA)  casD ← type I‑E CRISPR system Cascade subunit CasD
RA 2,880,763 G→A R96C (CGT→TGT)  casD ← type I‑E CRISPR system Cascade subunit CasD
RA 2,881,160 G→A A328V (GCT→GTT)  casC ← type I‑E CRISPR system Cascade subunit CasC
RA 2,912,293 G→A Y453Y (TAC→TAT relA ← GDP/GTP pyrophosphokinase
JC JC 2,913,392 IS2 (–) +5 bp coding (256‑260/2235 nt) relA ← GDP/GTP pyrophosphokinase
RA 2,955,349 C→T A219T (GCT→ACT)  recB ← exodeoxyribonuclease V subunit RecB
RA 3,025,288 C→T A313V (GCT→GTT)  xanQ → xanthine:H(+) symporter XanQ
RA 3,025,590 C→T P414S (CCC→TCC)  xanQ → xanthine:H(+) symporter XanQ
RA 3,032,624 C→T L420L (CTG→TTG)  uacT → urate:H(+) symporter
RA 3,037,203 C→T V302I (GTC→ATC)  recJ ← ssDNA‑specific exonuclease RecJ
RA 3,037,599 C→T A170T (GCA→ACA)  recJ ← ssDNA‑specific exonuclease RecJ
RA 3,038,660 C→T G55S (GGC→AGC)  dsbC ← protein disulfide isomerase DsbC
RA 3,041,643 C→T P111S (CCA→TCA)  ygfZ → folate‑binding protein
RA 3,052,074 C→T E82K (GAA→AAA)  ubiI ← 2‑octaprenylphenol 6‑hydroxylase
RA 3,060,787 C→T intergenic (+141/‑63) argP → / → scpA DNA‑binding transcriptional dual regulator ArgP/methylmalonyl‑CoA mutase
RA 3,061,674 C→T F275F (TTC→TTT scpA → methylmalonyl‑CoA mutase
RA 3,065,028 C→T A76V (GCC→GTC)  scpC → propionyl‑CoA:succinate CoA transferase
RA 3,081,667 C→T intergenic (‑32/‑246) tktA ← / → loiP transketolase 1/metalloprotease LoiP
RA 3,085,118 C→T S265N (AGC→AAC)  speA ← biosynthetic arginine decarboxylase
RA 3,086,235 G→A F18F (TTC→TTT
E17K (GAA→AAA) 
yqgG ←
yqgC →
protein YqgG
protein YqgC
RA 3,088,010 2 bp→TT intergenic (+150/‑273) metK → / → galP methionine adenosyltransferase/galactose:H(+) symporter
RA 3,093,876 C→T A126V (GCG→GTG)  yqgF → ribonuclease H‑like domain‑containing nuclease
RA 3,095,445 C→T R116R (CGC→CGT yggS → PLP homeostasis protein
RA 3,101,599 C→T A9T (GCA→ACA)  yggN ← DUF2884 domain‑containing protein YggN
RA 3,110,975 C→T G51E (GGA→GAA)  yghD ← putative type II secretion system M‑type protein YghD
RA 3,148,391 C→T V165V (GTC→GTT gpr → L‑glyceraldehyde 3‑phosphate reductase
RA 3,209,309 C→T A429V (GCG→GTG)  ttdT → L‑tartrate:succinate antiporter
RA 3,236,745 G→A G69D (GGT→GAT)  ygjQ → DUF218 domain‑containing protein YgjQ
RA 3,241,019 T→A F359Y (TTC→TAC)  sstT → serine/threonine:Na(+) symporter
RA 3,246,535 G→A intergenic (+13/‑117) exuT → / → exuR hexuronate transporter/DNA‑binding transcriptional repressor ExuR
RA 3,279,461 A→G H183R (CAT→CGT)  kbaZ → putative tagatose‑1,6‑bisphosphate aldolase 1 chaperone
RA 3,285,696 C→T F73F (TTC→TTT agaD → galactosamine‑specific PTS enzyme IID component
RA 3,336,411 2 bp→AT coding (83‑84/1260 nt) murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,403,159 G→A F58F (TTC→TTT csrD ← regulator of CsrB and CsrC decay
RA 3,413,728 G→A intergenic (‑263/‑136) envR ← / → acrE DNA‑binding transcriptional repressor EnvR/multidrug efflux pump membrane fusion lipoprotein AcrE
RA 3,436,697 G→A E60E (GAG→GAA trkA → NAD‑binding component of Trk potassium transporters
RA 3,476,285 G→A H52H (CAC→CAT yheO ← DNA‑binding transcriptional regulator YheO
RA 3,513,409 C→T S77N (AGC→AAC)  trpS ← tryptophan‑‑tRNA ligase
RA 3,515,325 G→A H197Y (CAC→TAC)  dam ← DNA adenine methyltransferase
RA 3,518,683 G→A P128S (CCG→TCG)  aroK ← shikimate kinase 1
RA 3,522,608 C→T R48R (CGG→CGA hofM ← DNA utilization protein HofM
RA 3,539,633 G→A G750D (GGT→GAT)  yhgF → putative RNA‑binding protein YhgF
RA 3,539,686 G→A A768T (GCA→ACA)  yhgF → putative RNA‑binding protein YhgF
RA 3,540,703 G→A E99E (GAG→GAA feoB → Fe(2(+)) transporter FeoB
RA 3,541,225 G→A A273A (GCG→GCA feoB → Fe(2(+)) transporter FeoB
RA 3,553,272 C→T S63L (TCG→TTG)  malT → DNA‑binding transcriptional activator MalT
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,642,547 G→A P196S (CCG→TCG)  rsmJ ← 16S rRNA m(2)G1516 methyltransferase
RA 3,643,308 G→A F625F (TTC→TTT prlC ← oligopeptidase A
RA 3,650,642 G→A A136T (GCG→ACG)  arsC → arsenate reductase
MC JC 3,653,231 Δ52,001 bp IS5‑mediated yhiS[dppB] 43 genes
RA 3,705,623 G→A L56L (CTC→CTT dppB ← dipeptide ABC transporter membrane subunit DppB
RA 3,706,152 G→A G518G (GGC→GGT dppA ← dipeptide ABC transporter periplasmic binding protein
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,708,813 G→A P555S (CCG→TCG)  eptB ← phosphoethanolamine transferase EptB
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,771,979 G→T intergenic (‑235/‑302) yibI ← / → mtlA DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component
RA 3,775,395 G→A L324L (CTG→CTA mtlD → mannitol‑1‑phosphate 5‑dehydrogenase
RA 3,805,480 C→T G157G (GGG→GGA waaP ← lipopolysaccharide core heptose (I) kinase
RA 3,805,830 C→T E41K (GAA→AAA)  waaP ← lipopolysaccharide core heptose (I) kinase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
JC 3,822,651 (CAGGAT)1→2 coding (252/2109 nt) spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,823,162 C→T H255Y (CAC→TAC)  spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,823,583 G→A G395E (GGG→GAG)  spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,824,443 G→A A682T (GCG→ACG)  spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,842,448 C→T intergenic (‑46/+7) yicN ← / ← adeQ DUF1198 domain‑containing protein YicN/adenine transporter
RA 3,854,927 G→A G336S (GGC→AGC)  emrD → multidrug efflux pump EmrD
RA 3,869,637 C→A L87L (CTC→CTA cbrA → colicin M resistance protein
RA 3,878,536 G→A Y528Y (TAC→TAT gyrB ← DNA gyrase subunit B
RA 3,898,467 C→T A149V (GCC→GTC)  cbrC → PF03691 family colicin E2 tolerance protein CbrC
RA 3,915,155 C→T G16S (GGC→AGC)  glmU ← fused N‑acetylglucosamine‑1‑phosphate uridyltransferase and glucosamine‑1‑phosphate acetyltransferase
RA 3,917,231 C→T G49S (GGC→AGC)  atpD ← ATP synthase F1 complex subunit beta
RA 3,918,209 C→T K19K (AAG→AAA atpG ← ATP synthase F1 complex subunit gamma
RA 3,919,941 C→T A155T (GCG→ACG)  atpH ← ATP synthase F1 complex subunit delta
RA 3,938,295 +T coding (69/993 nt) rbsR → DNA‑binding transcriptional dual regulator RbsR
RA 3,951,988 C→T intergenic (+1431/‑213) ilvX → / → ilvM uncharacterized protein IlvX/acetolactate synthase II subunit IlvM
RA 3,958,806 C→T N279N (AAC→AAT ilvC → ketol‑acid reductoisomerase (NADP(+))
RA 3,963,552 C→T G226G (GGG→GGA gpp ← guanosine‑5'‑triphosphate,3'‑diphosphate phosphatase
RA 3,965,392 C→T R80H (CGC→CAC)  rhlB ← ATP‑dependent RNA helicase RhlB
RA 3,970,539 C→T T136I (ACA→ATA)  wecB → UDP‑N‑acetylglucosamine 2‑epimerase
RA 3,976,185 G→A S347N (AGC→AAC)  wecE → dTDP‑4‑dehydro‑6‑deoxy‑D‑glucose transaminase
RA 3,979,089 C→T Y163Y (TAC→TAT wzyE → enterobacterial common antigen polymerase
RA 3,983,365 C→T I136I (ATC→ATT aslB → putative anaerobic sulfatase maturation enzyme AslB
RA 3,985,771 C→T E79E (GAG→GAA aslA ← putative sulfatase AslA
RA 3,986,329 C→T intergenic (‑322/‑103) aslA ← / → glmZ putative sulfatase AslA/small regulatory RNA GlmZ
RA 3,993,568 C→T P806S (CCG→TCG)  cyaA → adenylate cyclase
RA 3,996,049 T→A L156* (TTG→TAG)  yigA → DUF484 domain‑containing protein YigA
RA 4,011,802 C→T P243S (CCG→TCG)  bioP → biotin transporter
RA 4,015,369 C→T G267G (GGG→GGA ysgA ← putative dienelactone hydrolase
RA 4,015,879 A→G D97D (GAT→GAC ysgA ← putative dienelactone hydrolase
RA 4,046,937 C→T intergenic (‑335/‑29) yihG ← / → polA 1‑acylglycerol‑3‑phosphate O‑acyltransferase YihG/DNA polymerase I
RA 4,084,903 T→G N307T (AAC→ACC)  fdoG ← formate dehydrogenase O subunit alpha
RA 4,092,703 A→G F41L (TTC→CTC)  frvA ← putative PTS enzyme IIA component FrvA
RA 4,113,851 G→A H208Y (CAT→TAT)  fpr ← flavodoxin/ferredoxin‑NADP(+) reductase
RA 4,114,090 C→T G128D (GGT→GAT)  fpr ← flavodoxin/ferredoxin‑NADP(+) reductase
RA 4,118,719 G→A E69K (GAA→AAA)  zapB → cell division factor ZapB
RA 4,124,168 C→A G97* (GGA→TGA)  cytR ← DNA‑binding transcriptional repressor CytR
RA 4,135,729 C→T T632I (ACT→ATT)  katG → catalase/hydroperoxidase HPI
RA 4,137,967 G→A Q357* (CAG→TAG)  gldA ← L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase
RA 4,143,377 C→T S283L (TCA→TTA)  frwC → putative PTS enzyme IIC component FrwC
RA 4,159,493 C→T G433D (GGC→GAC)  sthA ← soluble pyridine nucleotide transhydrogenase
RA 4,161,248 G→T G61V (GGC→GTC)  fabR → DNA‑binding transcriptional repressor FabR
RA 4,164,123 C→G A162G (GCA→GGA)  btuB → cobalamin outer membrane transporter
RA 4,215,347 T→A intergenic (+138/‑131) metA → / → aceB homoserine O‑succinyltransferase/malate synthase A
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,282,332 (A)5→6 coding (744/1293 nt) yjcF ← pentapeptide repeat‑containing protein YjcF
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,341,309 G→A P107L (CCG→CTG)  melR ← DNA‑binding transcriptional dual regulator MelR
RA 4,392,997 G→A V357V (GTC→GTT queG ← epoxyqueuosine reductase
RA 4,393,552 G→A F172F (TTC→TTT queG ← epoxyqueuosine reductase
RA 4,394,231 G→A A56T (GCG→ACG)  nnr → NAD(P)HX epimerase/NAD(P)HX dehydratase
RA 4,396,373 C→T T103T (ACC→ACT amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,396,772 T→C F236F (TTT→TTC amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,399,673 G→A G141D (GGT→GAT)  miaA → tRNA dimethylallyltransferase
RA 4,425,688 C→A H57N (CAC→AAC)  priB → primosomal replication protein N
RA 4,425,696 C→A N59K (AAC→AAA priB → primosomal replication protein N
RA 4,447,298 G→A G1063S (GGT→AGT)  tamB → translocation and assembly module subunit TamB
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,532,409 T→G intergenic (‑758/+28) sgcX ← / ← yjhP putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP
RA 4,546,601 C→T I502I (ATC→ATT fimD → type I fimbriae usher protein
RA 4,612,957 G→A D183N (GAT→AAT)  yjjU → putative patatin‑like phospholipase YjjU
RA 4,613,247 G→A R279R (CGG→CGA yjjU → putative patatin‑like phospholipase YjjU
RA 4,613,740 G→A R87K (AGG→AAG)  yjjV → putative metal‑dependent hydrolase YjjV
RA 4,621,998 C→T A77V (GCC→GTC)  yjjJ → toxin YjjJ
RA 4,629,431 G→A T364T (ACC→ACT ettA ← energy‑dependent translational throttle protein EttA
RA 4,636,217 G→A A71T (GCG→ACG)  creB → DNA‑binding transcriptional regulator CreB
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,637,791 G→A A366T (GCG→ACG)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD
JC JC 4,640,418 IS30 (–) +2 bp coding (17‑18/141 nt) yjjY → uncharacterized protein YjjY

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 164552 164609 58 4 [3] [3] 4 hrpB/mrcB RNA‑dependent NTPase HrpB/peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB
* * ÷ NC_000913 1196333 1211436 15104 4 [3] [3] 4 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1978495 1979270–1978504 10–776 31 [0] [0] 13 insB5–insA5 insB5,insA5
* * ÷ NC_000913 2726218–2729227 2730810–2729571 345–4593 4 [3] [3] 4 rrlG–[rrsG] rrlG,gltW,[rrsG]
* * ÷ NC_000913 3423878–3424451 3424451 1–574 4 [3] [3] 5 [rrlD] [rrlD]
* * ÷ NC_000913 3708467 3708550 84 4 [0] [3] 4 dppA/proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
* * ÷ NC_000913 4287449 4312942 25494 23 [0] [0] 24 ytcB–[alsR] ytcB,nrfA,nrfB,nrfC,nrfD,nrfE,nrfF,nrfG,gltP,yjcO,fdhF,mdtP,mdtO,mdtN,ytcA,yjcS,alsK,alsE,alsC,alsA,alsB,[alsR]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994981 (0.060)28 (1.590) 23/254 0.0 96.5% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 = 1300693 NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 1299499 =NA (NA)14 (0.790) 12/254 0.2 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 1300694 = 0 (0.000)intergenic (+147/‑488) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1397238NA (NA)31 (1.600) 24/278 0.0 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1979271 =0 (0.000)13 (0.670) 9/278 0.6 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL
* ? NC_000913 2288919 =NA (NA)24 (1.240) 21/278 0.0 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 4287448 0 (0.000)intergenic (‑77/‑163) acs/ytcB acetyl‑CoA synthetase (AMP‑forming)/protein YtcB
* ? NC_000913 = 3653230NA (NA)23 (1.190) 19/278 0.0 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 4312943 = 0 (0.000)coding (49/891 nt) alsR DNA‑binding transcriptional repressor AlsR