Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,715	A→T	S377C (AGT→TGT)	ydiF →	putative acetate‑CoA transferase YdiF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,715	0	A	T	100.0%	42.7 / NA	16	S377C (AGT→TGT)	ydiF	putative acetate‑CoA transferase YdiF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (8/8);  total (8/8)

GTAAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTAT‑CACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGC  >  NC_000913/1776485‑1776849                                                                                                                                                                                                                                        |                                                                                                                                       gTAAAGGCGCGGTGGGGAATGTCGGCGGCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTAACCGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTAT‑CACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGAcc                                                                                                                      >  1:469505/1‑249 (MQ=255)                                                         ncccGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTAT‑CACGGAGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCa                                                                                        >  1:406392/2‑223 (MQ=255)                                                            cGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTAT‑CACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGAc                                                                                                                       <  1:118451/189‑1 (MQ=255)                                                            cGAGAAGAAGGTTGTGCTGATGACTTNATTCTGNNGGNAGAAACAGGTCCGATTGGCNGANNNANNNCNCAGGGNATNNCCTTTGGCGNGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTAT‑CNCGGTGGCGGNNTGGCTGTTTGTTATTTGTGTTNTGCNNANNTNGAc                                                                                                                       >  2:118451/1‑189 (MQ=255)                                                                        tgtgCTGATGTCTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTAT‑CACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGtn                                              <  1:299864/250‑2 (MQ=255)                                                                          tgCTGATGACTTTATTCTGACGGTAGAAACAGGTACGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTAT‑CACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGcc                                            >  1:424209/1‑250 (MQ=255)                                                                            ntgatgaCTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCAGAGGGGATCGCATTTGGCGAGAACGTGACTCCCCGTGCCATTCTGGATCTGACGTCCCAGTTTGATTTTTAT‑CACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGACGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACt                                         >  1:62233/2‑251 (MQ=255)                                                                                                   gAAACAGGTACGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATGCTGGATATGACGTCCCAGTTTGATTTTTAT‑CACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCCCGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGc                                                                       <  1:25202/198‑1 (MQ=255)                                                                                                       cgggTCCGATTGGCGGAATTACTTCACAGGGGATCGCCGTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTAT‑CACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACttn                                       <  1:63976/224‑2 (MQ=255)                                                                                                              gATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTAT‑CACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTg       >  1:439895/1‑251 (MQ=255)                                                                                                                   gCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGACCGTGAATAACCGTGCCATTCTGGATATGACGTCGCCGTATGATTTTTATTCA‑GGTGGCGGTCTGGATGTTTGTTATTAGTGTTTTGCTGAAGTCGACCAGCACGGAAACGTAGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGCAGAAAATCATTTTCTGCGGCCCATTAACTTCGGGc  >  1:456687/1‑251 (MQ=255)                                                                                                                                          gCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTAT‑CACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCggg   <  1:4682/227‑1 (MQ=255)                                                                                                                                                               aNNCGTGCCNNTCTGGATATGTCGTCCCAGTTTGATTTTTAT‑CACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGt                                                                                                              <  2:37555/99‑1 (MQ=255)                                                                                                                                                                cccGTGCCATTNTNGATATGACGTCCCAGTTTGAGTTTTAT‑CACGGTGGCGGTCTGGATGTTNGTTATTTGTGNTTTNNTGAANTNGNCCAGCACGGTAACGTCGGCGTGCATAAATNCAATGGTAAAATCATGGGCACCGNNGGNNNTANNGatat                                                   <  2:378230/157‑1 (MQ=255)                                                                                                                                                                cccGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTAT‑CACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGatat                                                   >  1:378230/1‑157 (MQ=255)                                                                                                                                                                                      gTCCCAGTTTGATTTTTAT‑CACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACGGCggg   <  1:137945/183‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                       GTAAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTAT‑CACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGC  >  NC_000913/1776485‑1776849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑