Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,539,686	G→A	A768T (GCA→ACA)	yhgF →	putative RNA‑binding protein YhgF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,539,686	0	G	A	100.0%	32.6 / NA	15	A768T (GCA→ACA)	yhgF	putative RNA‑binding protein YhgF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/4);  total (11/4)

TGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATT  >  NC_000913/3539445‑3539900                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                       tgtgGAAGATCCGCATTCCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACAATGGGc                                                                                                                                                                                                                <  1:21337/250‑1 (MQ=255)                             gCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCACAACAACCGCCCGGCAGCCAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACAATGGGCAAAAAACg                                                                                                                                                                                                        >  1:209521/1‑230 (MQ=255)                                                                                                                    ggCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACAATGGGCAAAAAACGTTAAACGCCCGTATTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCa                                                                                                                                        >  1:409898/1‑207 (MQ=255)                                                                                                                                                          nccGCAAAACAACCGCCCGGCAGCCAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTc                                                      >  1:403944/2‑251 (MQ=255)                                                                                                                                                          cccGCAAAACAACCGCCCGGCAGCCAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCaacgaac                                                                                                                    >  1:390610/1‑189 (MQ=255)                                                                                                                                                           ccGCAAAACAACCGCCCGGCAGCCAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGGTGCGCTGGCGGCGACAATGGGCAAAAAACGTTAAACGCACGGACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCaac                                                                                                                        >  1:79486/1‑184 (MQ=255)                                                                                                                                                                     cccGCCCGGCAGCCAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACTGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACTCCTTGCCTGACATAAAAGTGCCGGAGAATATCGCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTc                                           <  1:357486/250‑1 (MQ=255)                                                                                                                                                                           cGGCAGCCAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGACATCGTAGGGCAGATACGGCGTTCACGCCGCCTCTGGCaac                                                                                                                        >  1:215951/1‑168 (MQ=255)                                                                                                                                                                            ggCATCCAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACAATGGGCAAAAAACGGTAAACGCCCGTACTGGCCTACGGTTCGAAGTTGCACGAAATGGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACAGAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGc                                       <  1:359558/248‑1 (MQ=255)                                                                                                                                                                                 ncAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTg                                                                                                                                                                      >  1:315084/2‑116 (MQ=255)                                                                                                                                                                                 ccAAACCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTg                                                                                                                                                                      <  2:315084/116‑1 (MQ=255)                                                                                                                                                                                   aaaCCACGCGATCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACCATGGGCAAAAAACGTTAAACGcc                                                                                                                                                                                               >  1:253682/1‑89 (MQ=255)                                                                                                                                                                                                gTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACAATGGGCAACACACGTTCAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGCAATCGTAGGGCAGATAAg                                                                                                                                               >  1:187858/1‑124 (MQ=255)                                                                                                                                                                                                      cgcAGACTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGACAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGa                                                                                                         >  1:386168/1‑156 (MQ=255)                                                                                                                                                                                                                                          tGGCGGCGACAATGGGCAAAAAACGTTAAACGCCCGTACTGGCATACGGTTCGAATTTGCACGCCATCGTAGGGCAGATACGGCGTTCACGCCGCCTCTGGCAACGAAAGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTTACAACTGAAACCttaatt  >  1:359319/1‑223 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                       TGTGGAAGATCCGCATACCGTGGTGAAAGCGGGCGACATTGTGAAGGTGAAAGTGCTGGAAGTGGATCTTCAGCGTAAACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATT  >  NC_000913/3539445‑3539900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑