Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	555,459	C→T	Y283Y (TAC→TAT)	cysS →	cysteine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	555,459	0	C	T	100.0%	47.9 / NA	18	Y283Y (TAC→TAT)	cysS	cysteine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/5);  total (13/5)

TGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGACGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGGCGCG  >  NC_000913/555245‑555701                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   tGAACTGCAAGCAGCTGGGAAACCACTTTGAAATCCACGGCCGCGGTTCACACATGATGCTCCCGATCCACGAAACCGACATCGCGCAGCCCACCTGTCCCCATGATGGCCAGTATGTGAACTACTGGATGCACTCGGGGACGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTACCTTCTTTACCGAGCGCGACGTGCTGAAATATTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCg                                                                                                                                                                                                                >  1:197058/1‑251 (MQ=255)       acAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATATTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCAc                                                                                                                                                                                                          >  1:143955/2‑251 (MQ=255)                  ggTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCAACAAGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCCGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGCTGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATATTACGACGCGGAAACCGTGCGTTACTTCCTGAt                                                                                                                                                                                                                    >  1:371923/1‑230 (MQ=255)                                                             tCCCGCACCACGAAAACGAAATCGCGCAGTCCAACTGTGCCCATGATGGTCAGTATGTGAACTCCTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATATTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGaa                                                                                                                                                                                         >  1:162700/1‑214 (MQ=255)                                                                            acgaaaTCGCGCCGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTCACTTCTTTACCGTGCGCGCTGTGCTGAAATATTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACt                                                                                                                                                                                       >  1:352294/1‑201 (MQ=255)                                                                                    gcgcAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATATTACGACGCGGAAACCGCGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACActgcgct                                                                                                                             >  1:500349/1‑251 (MQ=255)                                                                                                                                   gCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGTGTAACTTCTTTACCGTGCGCGATGTGCTGAAATATTACGACGCGGAAACCGTGCGTTACTTCCt                                                                                                                                                                                                                       <  1:15847/114‑1 (MQ=255)                                                                                                                                      cTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATATTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCa                                                                                                                                                                                                           >  1:177962/1‑123 (MQ=255)                                                                                                                                       tCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATTGCTGGGTAACTTCTTTACCCTGGGCGAAGGCCTGACATATTACGACGCGGAAACCGAGGCTTACTTCCCGATGTCGGGTCACTATCGCAGACAGTTGGAATACCGAGACAAGAACCTGCAGCCGGCGCGTCCCGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAACCCGTTGCGACTGCCGGTGGCGAAGCGCTTTAAgc                                                                          >  1:269446/1‑251 (MQ=255)                                                                                                                                        cGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATATTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAgcg                                                                         >  1:121367/1‑251 (MQ=255)                                                                                                                                                                                        acgtcttTCCCGTGCGCGATGTGCTGAAATATTACGACGCGGAAACCGTGCGTTGCTGGCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGACCCTGAAGCAGGCGCGTGCGGCGCTGGAGCGtcgc                                                                                                                                        <  1:220471/137‑3 (MQ=255)                                                                                                                                                                                        aCTTCNTTACCNTNNGCGATGTGCTGAAATATTANGACGCGGAATCCGTGNGTTANTTCCtnctntn                                                                                                                                                                                                                 >  2:220471/1‑61 (MQ=255)                                                                                                                                                                                            cttTACCGTGCGCGATGTGCTGAAATATTACGACGCGGAAACCGTGCGTTAATTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAg                                                                                                                                                                                   >  1:215111/1‑93 (MQ=255)                                                                                                                                                                                                     gnncGATGTNNTGAAATATTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGaa                                                                                                                                                                    <  2:27129/99‑1 (MQ=255)                                                                                                                                                                                                      cgcgATGTGCTGAAATATTACNNCGCGGACACNGTGCGTTACTTCCTGATGTCGGGCCCCTATCGCAGCCAGTTGAACTACAGCGANGAGAACCTGNNGCNGGCGCNTNNNGNNNTGNAGCGTCTCTACACTGCGCTNCGNNGCACAGNTAAAACCTTTGCGCCTGCCGGTGGCg                                                                                       <  2:116515/175‑1 (MQ=255)                                                                                                                                                                                                      cgcgATGTGCTGAAATATTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCg                                                                                       >  1:116515/1‑175 (MQ=255)                                                                                                                                                                                                               cTGAAATATTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGACGGTTGACATACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACCCTGCGCTGAGCGGCACAGATCAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGACGAGGATTTCAACACACCGGAAGCCTATTCCGTACTGTTTGATATGGagcg  >  1:273302/1‑251 (MQ=255)                                                                                                                                                                                                                 gAAATATTACGACGCGGAAACCGTGCGTTACTGCCTGAGTTCGGGCCACTATCGCAGCCAGTTGAACGACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACActgcgctgc                                                                                                                           <  1:416069/128‑1 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   TGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGCGATGGACGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTTGATATGGCGCG  >  NC_000913/555245‑555701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑