Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,415,485	G→A	G247S (GGC→AGC)	pta →	phosphate acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,415,485	0	G	A	100.0%	40.7 / NA	16	G247S (GGC→AGC)	pta	phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/12);  total (4/12)

AACTGAACGCACCGGTTGATGAACAGGGTCGTACTCGCCCGGATCTGTCCGAGATTTTCGACGACTCTTCCAAAGCTAAAGTAAACAATGTTGATCCGGCGAAGCTGCAAGAATCCAGCCCGCTGCCGGTTCTCGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTCCGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTG  >  NC_000913/2415267‑2415727                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   aaCTGAACGCACCGGTTGATGAACAGGGTCGTACTCGCCCGGATCTGTCCGAGATTTTCGACGACTCTTCCAAAGCTAAAGTAAACAATGTTGATCCGGCGAAGCTGCAAGAATCCAGCCCGCTGCCGGTTCTCGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAAGCGACATCAATACTCGCCGCGTTAAATCCGTc                                                                                                                                                                                                                    <  1:180015/251‑1 (MQ=255)                   atgaACAGGGTCGTACTCGCCCGGATCTGTCCGAGATTTTCGACGACTCTTCCAAAGCTAAAGTAAACAATGTTGATCCGGCGAAGCTGCAAGAATCCAGCCCGCTGCCGGTTCTCGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAAGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACgcagc                                                                                                                                                                                                  >  1:275590/1‑251 (MQ=255)                                                                                      cAATGTTGATCCGGCGAAGCTGCAAGAATCCAGCCCGCTGCCGGTTCTCGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAAGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCAGATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGa                                                                                                                                                    <  1:458196/230‑1 (MQ=255)                                                                                            tGATCCGGCGAAGCTGCAAGAATCCAGCCCGCTGCCGGTTCTCGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAAGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCTGTTCTCTGCTGGTGACTTCCGCAGan                                                                                                                                         <  1:404508/235‑2 (MQ=255)                                                                                                 cGGCGAAGCTGCAAGAATCCAGCCCGCTGCCGGGTCTCGGCGCTGTGCCGGGGAGCTTTGAGCTGATCGCGACTCGTGCGATCGATATGGCGCGCCACCTGAATGCGACCATCATCAACGAAAGCGACATCAATGCTCGCCTCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGc                                                                                                                                                                                           <  1:467871/180‑1 (MQ=255)                                                                                                            cAAGAATCCAGCCCGCTGCCGGTTCTCGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAAAGAAAGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTc                                                                                                                                                                                                              >  1:212338/1‑150 (MQ=255)                                                                                                                 aTCCAGCCCGCTGCCGGTTCTCGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAAGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTgg                                                                                                                                                                                  <  1:30085/173‑1 (MQ=255)                                                                                                                                      cGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAAGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTCCGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTg                                                                               <  1:8620/251‑1 (MQ=255)                                                                                                                                                       gCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCAGCATCAACGAAAGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTGCTGCGCACGCAGCATTCCGCACACGCTGGAGCACTGCCGTGCCGGGTCGCTGCTGGTGACTTCCGCAGACCGTCCTGACGTGCtggtg                                                                                                                       <  1:344108/194‑1 (MQ=255)                                                                                                                                                                ngATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAAGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTCCGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTAcac                                                      >  1:308717/2‑248 (MQ=255)                                                                                                                                                                    cgcgACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAAGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGc                                                                                                                                                                               <  1:187694/125‑1 (MQ=255)                                                                                                                                                                     gcgACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAAGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTCCGCAGACCGTCCTGACGTGCTGGTGGCCGc                                                                                                                  <  1:449464/185‑1 (MQ=255)                                                                                                                                                                                           gttcagacgtggctcttccgatcttcaACGAAAGCGACATCAATACTCGGCGCGTGAAATCCGTCACGTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGGTCTCTGCTGGTGACTTCCGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGc                          <  1:448350/227‑1 (MQ=255)                                                                                                                                                                                                cACCTGAATGCGACCATCATCAACGANAGCGACNNCANTACTCGCCGCGTTAAATCCNTCNNNTNNNGNACACGNAGNNTTCCGCACANGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTCCGCAGACCGTCCTGNCGTGCTGGTNNCCGCTTGCCTGGCAGCCATGANCGGNNTNNANATCNGt                                                                                >  2:107640/1‑192 (MQ=255)                                                                                                                                                                                                     gAATGCGACCATCATCAACGAAAGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCAGTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTCCGCAGACCGGCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGc                                                                              <  1:65045/189‑1 (MQ=255)                                                                                                                                                                                                                    caACGAAAGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTCCGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCtn  <  1:316083/250‑2 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   AACTGAACGCACCGGTTGATGAACAGGGTCGTACTCGCCCGGATCTGTCCGAGATTTTCGACGACTCTTCCAAAGCTAAAGTAAACAATGTTGATCCGGCGAAGCTGCAAGAATCCAGCCCGCTGCCGGTTCTCGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTCCGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTG  >  NC_000913/2415267‑2415727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑