| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,721,136 | C→T | L38F (CTT→TTT) | ydhI → | DUF1656 domain‑containing protein YdhI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,721,136 | 0 | C | T | 94.4% | 40.7 / ‑6.6 | 18 | L38F (CTT→TTT) | ydhI | DUF1656 domain‑containing protein YdhI |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (5/12); minor base A (1/0); total (6/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
CGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGCTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGC > NC_000913/1720964‑1721354 | cGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCTGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTg < 1:400309/250‑1 (MQ=255) cACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGttt > 1:34712/1‑251 (MQ=255) cTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTacac > 1:143394/1‑180 (MQ=255) naaTATTTATTACTCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGc > 1:131350/2‑200 (MQ=255) aCGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGATTGGATTCGTCATCTGGTTTGTCGTa < 1:5515/154‑1 (MQ=255) cGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTg < 1:325254/215‑1 (MQ=255) aGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGNTGGNNNNNCg > 2:305836/1‑144 (MQ=255) aGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTggn < 1:305836/214‑2 (MQ=255) ggttATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGAAAGACCGAGTCCTTTACTCCGCTTTTCAACGCATTCGCGTTAGGATTCGTCATCTGGATTGTCGTACACCGACTGATTCGTGGCTGGATCTACGCCGGTGAAATCTGGCATCACTTGTTAATGGATTTATCGATGTTTGCGATTTGCGTTTGACTTGCTCTGGCAATACTGATTGTGTGGTAACTATGTCAATTAAAACAAt > 1:457238/3‑251 (MQ=255) cTCAAGGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCGCCGTTGTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTTTCGTACGCCGCTCGCTGCTTTGATGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGttt < 1:474825/180‑1 (MQ=255) cAATGCAANANNNTNNCCCTTACAAGATCTGGTGTTCAGTGCGTCCGTCTACTTTCCTCCNTTTTTCAAAGCATTCNCGNTTGGNTTCNTCATCTGGTTTGt > 2:358223/1‑102 (MQ=255) cAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCAGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGt < 1:358223/102‑1 (MQ=255) cgtccgtcTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTc < 1:119890/78‑1 (MQ=255) tccgtcTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTgg < 1:407652/84‑1 (MQ=255) tccgtcTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTcaan < 1:57738/214‑2 (MQ=255) ttttCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACTCCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATg < 1:71684/101‑1 (MQ=255) cATCTGGTTTGTCGTACACCGCCTGCTTCGTGTCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTTTAGCGGTAGTTGCTGTGCTTGCCGGATGGTGGCTGTGGAATTATTACATGc < 1:195928/226‑1 (MQ=255) tCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTggn < 1:94126/57‑2 (MQ=255) | CGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGCTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGC > NC_000913/1720964‑1721354 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |