| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,140,714 | G→A | A228T (GCG→ACG) | flgL → | flagellar hook‑filament junction protein 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,140,714 | 0 | G | A | 94.7% | 47.5 / ‑6.0 | 19 | A228T (GCG→ACG) | flgL | flagellar hook‑filament junction protein 2 |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (11/7); total (12/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTCGATGGTGATAGGGCACACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATGCAGCAAACGGCAT > NC_000913/1140535‑1140921 | gttagATGGTGATAGTGCACACGGGTGACAAAATTTTCGACAGTATTACCAGCAAAGGGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAAGCTTTTTGCCATGCTGGATAGGGCAATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGACGGTATATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTAggn < 1:59317/247‑2 (MQ=255) cAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGATTCTGAAACAAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGACGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTAGGAACGCAGCTGAACGAACTGGAGTCGCTgg > 1:506637/1‑251 (MQ=255) gACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGACGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTAg > 1:74820/1‑211 (MQ=255) cGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGACGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGAt > 1:412111/1‑223 (MQ=255) gcgcTTCGGAAACCAATCTTTTGGCCAGGCGGGAGAGGGCCATCGCAGCCCTGAAAACCCCGGTCGCGGATAGCTAAGCTGATAAAGAAACCGCCGCTGCGACGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCGGACTGTTCGCGCGGAATTAGGCACGCAGCTGAACGAACTgn < 1:46602/192‑2 (MQ=255) gAAACAAATTTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAACCGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGACGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTg < 1:3576/250‑1 (MQ=255) ccATGCTGGATAGTGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGACGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGcn < 1:98295/225‑2 (MQ=255) ccATGCTGGATAGTGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGACGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTNcgcnnn > 2:305934/1‑137 (MQ=255) ccATGCGGGATAGTGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGACGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATcn < 1:305934/196‑2 (MQ=255) ngATAGTGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGACGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTAGGCACGCAGCTga > 1:122798/2‑152 (MQ=255) cgcccgaaaaaCGCCGGTCGCGGATAGCGAAGGGGATAAAAAAACCGCCGCTGCGACGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGACAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCAGCTTACATCATgc < 1:417755/244‑1 (MQ=255) ccTGAAAACGCCGGCCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTCGATAAAACCAACAGCGGAATGACAAACTAGCTGAACAATGAGCTGACAGTTCGCGCGGACTTAGGCACGCAGCTGAACGAACt > 1:460563/1‑141 (MQ=255) gAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGACGTTAGATAAAAACAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATAGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTAt > 1:473239/1‑228 (MQ=255) aCGCCGGGCGCGGATAGAGAAGCGAATAAAGAAACCGCCGCTGCGACGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGGTCGCGCGGAATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCg < 1:134541/160‑1 (MQ=255) ggTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGACGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTAGGCACGCAGCTgaac > 1:281241/1‑124 (MQ=255) gCGAAGCGNNTANNGNANCCGCCNCTGCGACGTTAGATAAAACCAACCGCGGACTGAAAAACTCg > 2:327006/1‑65 (MQ=255) gCGAAGCGGATAAAGACACCGCCGCTGCGACGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGAGGACTGGAATGCAACTATTTCATCTTACATCATGCAGCAAACGGCAt > 1:112875/1‑237 (MQ=255) ggATAAAGCAACCGCCGCTGCTACGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACaa > 1:390700/1‑66 (MQ=255) gcTGCGACGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACACTGTGCTGACTGTTcgcgcg > 1:268624/1‑69 (MQ=255) | GTTCGATGGTGATAGGGCACACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATGCAGCAAACGGCAT > NC_000913/1140535‑1140921 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |