Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,393,552	G→A	F172F (TTC→TTT)	queG ←	epoxyqueuosine reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,393,552	0	G	A	100.0%	30.1 / NA	14	F172F (TTC→TTT)	queG	epoxyqueuosine reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/4);  total (10/4)

GGCAGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGCCGAGTTTGGGGTTTTTC  >  NC_000913/4393320‑4393789                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                              ngCAGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTcg                                                                                                                                                                                                                             >  1:92754/2‑251 (MQ=255)                                                               ccTTCACGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCAAACGGgcg                                                                                                                                                               >  1:402845/1‑251 (MQ=255)                                                                                                               gTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTACTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCCGCAATTCGCCTAAAAAAAAGACCGAACAGGCCTCGCGATTGAGGATAAGTGAGTGATTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAAAGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAACTTCAGCGAAac                                                                                                               >  1:296306/1‑251 (MQ=255)                                                                                                                                      ngTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAg                                                                                                                                                                                                                     >  1:49581/2‑126 (MQ=255)                                                                                                                                                              cACGCATTTGCCGCATACTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCg                                                                                                                                                                                         >  1:417410/1‑130 (MQ=255)                                                                                                                                                                         ngCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTACAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCGGAAGTTtgtg                                                      >  1:132222/2‑250 (MQ=255)                                                                                                                                                                                          ggTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGAGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCCGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTACAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGa                                                                            >  1:454229/1‑211 (MQ=255)                                                                                                                                                                                                tCCACGGGCAGCGGAAGATCGACCAGCAATGCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGTATAAGTGAGTGCTTACCGGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCg                                                                                                                                                           <  1:272442/126‑1 (MQ=255)                                                                                                                                                                                                       gCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCg                                                                                                                                                                                         >  1:136846/1‑89 (MQ=255)                                                                                                                                                                                                          gCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGt                                                 <  1:15687/222‑1 (MQ=255)                                                                                                                                                                                                                        aGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGCCAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACAAAGCCGAGTTTGGGGtt      >  1:266358/1‑251 (MQ=255)                                                                                                                                                                                                                         gCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGTATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTGTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAg                                                                                                                     <  1:328787/139‑1 (MQ=255)                                                                                                                                                                                                                         gCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTGCCTGTCCAGCCGAACCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGACTCATCTCGCCAAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACTGCCCAGCGCATAACTGCTAACATAACCGCGTTTGGGGt       >  1:263129/1‑249 (MQ=255)                                                                                                                                                                                                                                  cTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAAGCATCTCGCCCAGCTTTTTGAGGCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGCCGAGTTTGGGGTTTTTc  <  1:76546/245‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                              GGCAGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGCCGAGTTTGGGGTTTTTC  >  NC_000913/4393320‑4393789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑