| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,041,643 | C→T | P111S (CCA→TCA) | ygfZ → | folate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,041,643 | 0 | C | T | 95.7% | 54.4 / ‑5.7 | 23 | P111S (CCA→TCA) | ygfZ | folate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (10/12); total (10/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
GCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAA‑TATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGG > NC_000913/3041400‑3041848 | gCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCCGATGTCAGCAAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCCTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGTCAgacg > 1:269291/1‑251 (MQ=255) ccaccaTTACTGACGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGCTGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAA‑TATGCGGTATTCTCTAAAGTGACCATCGCGTCAgacgac > 1:391508/1‑251 (MQ=255) cAGGTGACAGCAGAGGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAA‑TATGCGGTATTCTCTAAAGTGACCATCGCGTCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgcgn < 1:146628/251‑2 (MQ=255) gcagATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAA‑TATGCGGTATTCTCTAAAGTGACCATCGCGTCAGACGACGAGCGTGTGC‑ACTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGc > 1:11122/1‑250 (MQ=255) cagATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAA‑TATGCGGTATTCTCTAAAGTGACCATCGCGTCAGACGACGAGCGTGTGCTGCTTGGTGttn < 1:101868/222‑2 (MQ=255) cAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGGAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAA‑TATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTgg < 1:26129/210‑1 (MQ=255) ccAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAA‑TATGCGGTATTCTCTAAAGTGACCATCGCGTCAGACGACGAGCGTGTGCTGCTTGgtgt > 1:216225/1‑210 (MQ=255) cGCCAAAGGTAAAATGTGGAGCTATTGACGTCTGTTCCGCGACGGCGATGGGTTTGCATGGATTGAACGGTGCAGCGGGGGTGAACCGCAGCTGACTGAACTGAAAAAA‑TATGCGGTATGCTCGAAAGTGCTCATCGCGTCAGTCGTCGAGCGGGTGCTGCTTGGGGTTGCCGGTTTGCGGGCGCGCTCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAGGAAGgcg < 1:329646/251‑1 (MQ=255) cGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAA‑TATGCGGTATTCTCTAAAGTGACCATCGCGTCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCa > 1:500088/1‑180 (MQ=255) gCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAA‑TATGCGGTATTCTCTAAAGTGACCATCGCGTCAgac < 1:328218/144‑1 (MQ=255) tCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAA‑TATGCGGTATTCTCTAAAGGGACCATCGCGTCAGACGACGAGCGTGTGCTGCTTgg < 1:428638/135‑1 (MQ=255) cgcgACTGCGATGGCTTTGCATGGATGGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAA‑TATGCGGTATTCTCTAAAGTGACCATCGCGTCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAGCAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACg < 1:465431/251‑1 (MQ=255) gATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAA‑TATGCGGTATTCTCTAAAGTGACCATCGCGTCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCa < 1:6466/134‑1 (MQ=255) ggCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAA‑TATGCGGTATTCTCTAAAGTGACCATCGCGTCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCTCCGCGCTGGCAAATCTCTTTa > 1:410531/1‑160 (MQ=255) ggATTGAACGGCGCAGCGTGCGGGAACCGCAGCTGACTGAACTGAAAAAA‑TATGCGGTATTCTCTAAAGTGACCATCGCGTCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCNCTGNNNNNtc > 2:319068/1‑144 (MQ=255) gAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAA‑TATGCGGTATTCTCTAAAGTGACCATCGCGTCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGtt > 1:418843/1‑112 (MQ=255) aGNNNNNTGANCTGAAAAAA‑TATGCGGTATTCTCTAAAGTGACCATCGCGTCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAAc < 2:311907/144‑1 (MQ=255) gactgaACTGAAAAAA‑TATGCGGTATTCTCTAAAGTGACCATCGCGTCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGcgcg < 1:42381/251‑1 (MQ=255) actgaactgaAAAAA‑TATGCGGTATTCTCTAAAGTGACCAGCGGGTCAGACGACGTGCGTGTGCTGCGTGGTGTTGCCGGTTTGCAGGCGCGCGCCGCGCTGGCAAAACTCTTTATCGAACTGCCTTCGAAAGAAAAACAGGGATTCAAAGATGGCGCGACCGCTTTGCTATGGGTGGAACACCCGGCAGAATGTTGCCTGAGCGTAACCGATGGATCGTCTTCTAATATGCTGACCGATAAACTGCGCgg < 1:423365/251‑1 (MQ=255) ctgaactgaaAAAA‑TATGCGGTATTCTCTAAAGTGACCATCGCGTCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgc > 1:198754/1‑92 (MQ=255) ngncanaa‑TATNCGGTNTTCTCTAAAGTGACCNTCGCGTCAGACGACGAGCGNNTNCTGCTNGgtgt < 2:216225/63‑1 (MQ=255) ctctAAAGTGACCATCGCGTCAGACGACGAGCGTGTGCTGCTCGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGc > 1:79006/1‑162 (MQ=255) agcgcgTCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCCGTTTTCAGGCGCGTTCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaag < 1:76039/92‑1 (MQ=255) | GCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAA‑TATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGG > NC_000913/3041400‑3041848 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |