Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,775,395	G→A	L324L (CTG→CTA)	mtlD →	mannitol‑1‑phosphate 5‑dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,775,395	0	G	A	100.0%	31.1 / NA	12	L324L (CTG→CTA)	mtlD	mannitol‑1‑phosphate 5‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/4);  total (8/4)

CAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAGCGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATAACAGATTGTCAGATATGCAGGCAACAAT  >  NC_000913/3775227‑3775636                                                                                                                                                                         |                                                                                                                                                                                                                                                  cAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGACGTCAGCCACTGCGTCAACTGCGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTAATTGAAGGTATTGCCGCTGCACTGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGAc                                                                                                                                                                  >  1:260214/1‑250 (MQ=255)               aCATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGGGCTGGCGACCGTTTGATCAAGCCACTGCTCGGTACGCTGGAAGATGGGCTGCCACATAAAAACCTAATTGAAGGTATTGCCTCTGCAATGCACTTCCGCAGTGAAgatga                                                                                                                                                                                                       <  1:186693/199‑1 (MQ=255)                     aGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTAATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTg                                                                                                                                             <  1:435068/251‑1 (MQ=255)                                                                                                    gAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTAATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAGCGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAAtggt                                                              >  1:14393/1‑251 (MQ=255)                                                                                                                       aTCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCCCATAAAAACCTAATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAGCGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACAcc                                           >  1:250418/1‑251 (MQ=255)                                                                                                                       aTCAAGCCACTGCGCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTAATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGAGCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAGCGAGGTTGTATCCTAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACAcc                                           <  1:436350/251‑1 (MQ=255)                                                                                                                                ntGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTAATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATcc                                                                                                                                                                                                    >  1:122969/2‑89 (MQ=255)                                                                                                                                       gTACGCTGGAATATGTTCTGCCACATAAAAACCTAATTGAAGGTATTGCCGATGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGATAAAGGTCCGCAGGCGGAGCTGGCACAGATTTCCGGTCTTGATGCCAACAGCGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAgcaatgc                                                                       >  1:349837/1‑207 (MQ=255)                                                                                                                                                          nccACATAAAAACCTAATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAGCGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGAAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATAACAGATTGTCAAATATGccagc        >  1:297426/2‑247 (MQ=255)                                                                                                                                                                tAAAAACCTAATTGAATGTATTGCCGCTGCAATGCACTTCCGCAGTTAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCa                                                                                                                                                       <  1:211141/102‑1 (MQ=255)                                                                                                                                                                tAAAAACCTAATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGGTCAGGAACTGGCAGCACTGATCGATGACAAAGGACCGAAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCACAAGCGAGGTTGTATCCGAGGAGGTCACCGCTTATAAAGCAATGCCATACTGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATAACAGATTGTCAGATATGCAGGCAACAAt  >  1:223860/1‑251 (MQ=255)                                                                                                                                                                tAAAAACCTAATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCAGCCGGCTCAGGCACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAGCGAGGTTGTATCCGAGGCGGCAAACGCTTATAAAGCCATGCAATAATGGGGGACCAGGCGCAGGacac                                            >  1:283863/1‑209 (MQ=255)                                                                                                                                                                         |                                                                                                                                                                                                                                                  CAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGAACTGGCAGCACTGATCGCTGACAAAGGTCCGCAGGCGGCGCTGGCACAGATTTCCGGTCTTGATGCCAACAGCGAGGTTGTATCCGAGGCGGTAACCGCTTATAAAGCAATGCAATAATGGTGGACCAGGCGCAGGACACCCTGCGCCCGAATAACAGATTGTCAGATATGCAGGCAACAAT  >  NC_000913/3775227‑3775636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑