Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,654,454 G→A T160T (ACC→ACT)  sseB ← protein SseB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,654,4540GA91.3% 51.4 / ‑5.2 23T160T (ACC→ACT) sseBprotein SseB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (11/10);  minor base C (2/0);  total (13/10)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGTTCATCGCCAGGTAATGTATCGGTCGCTACACTTCCCGTCGCCTGAATAATTTCTTCGATATCACCATCGGCTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAATCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGATTTCACGCGGCATAAATTCTTTACCGGTTGGCAGTTTGGCATTAAGGAAGAGCGTTTCGCCAAGTGTCATCTCAAACAGCGTGCGAACGGGCATTACGACAAATGCCTGTTCGTCTTCAAC  >  NC_000913/2654220‑2654699
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     
cgcAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTAACCTGACAAATATCGATTGGTTCATCGCCAGGTAATGTATCGGTCGCTACACTTCCCGTCGCCTGAATAATTTCTTCGATATCACCATCGGCTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAACGTGGTGAGTGaatca                                                                                                                                                                                                                                        >  1:75573/1‑250 (MQ=255)
               aataaAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGTTCATCGCCAGGTAATGTATCGGTCGCTACACTTCCCGTCGCCTGAAAAAGTTCTTCGATATCACCATCGGCTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGTTTTTAAATAAAGTGGTGAGTGAATCAATCATTTGTGCTggc                                                                                                                                                                                                                         <  1:504927/250‑1 (MQ=255)
                               ccTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGTTCATCGCCAGGTAATGTATCGGTCGCTACACTTCCCGTCGCCTGAATAATTTCTTCGATATCACCATCGGCTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAAGTGGTGAGTGAATCAATCATTTGTGCTGGCGGCTCTg                                                                                                                                                                                                                  <  1:172337/241‑1 (MQ=255)
                                           tttttCACCTGACAAATATCGATTGGTTCATCGCCAGGTAATGTATCGGTCGCTACACTTCCCGTCGCCTGAATAATTTCTTCGATATCACCATCGGCTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAAGTGGTGAGTGAATCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAgn                                                                                                                                                                                              <  1:123639/249‑2 (MQ=255)
                                                                             cAGGTAATGTATCGGTCGCTACACTTCCCGTCGCCTGAATAATTTCTTCGATATCACCATCGGCTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAAGTGGTGAGTGAATCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGTCTGCTCAGc                                                                                                                                                          <  1:157342/251‑1 (MQ=255)
                                                                                                  cacTTCCCGTCGCCTGAATAATTTCTTCGATATCACCATCGGCTGCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCGTCAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTGTTAAATAAAGTGGTGAGTGAATCAATCATTTGTGCTGGCGGCTCTGCGACTTcc                                                                                                                                                                                                          <  1:246512/182‑1 (MQ=255)
                                                                                                        ncGTCGCCTGAATAATTTCTTCGATATCACCATCGGCTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATACAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAAGTGGTGAGTGAATCAATCATTTGTGCTGGCGGcta                                                                                                                                                                                                                    >  1:100363/2‑165 (MQ=255)
                                                                                                                   ataatTTCNTNNNTNNCACCATCGGCTTCAATGCCCATAAGTAAATTAGGCTGTTCCTCTNCGTTCTCTTTAATTGNACNAATANAAGNACGCTTCACCGGCTTAATGGTTTTAAATAAAGTGGTGAGTGAATCAATCATTTGTGCTGGCGGCTTTGCGACTTCCGATAATATCAGCCATTCCCCGCCTTCCAGNATTNCCTGGNNGNTCAGCGNNnttnnnnnnnncnnnncc                                                                                                                                     >  2:345742/1‑219 (MQ=255)
                                                                                                                   ataatTTCATCGATATCACCATCGGCGTCAATGCCAATAAGTAAATTAGGCTGTGCATCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAAGTGGTGAGTGAATCAATCAGTTGTGCTGGCGGCTCTGCGACTTTCTATAATATCAGCGATTCACCTCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCTCCAATc                                                                                                                                     <  1:345742/234‑1 (MQ=255)
                                                                                                                      atTTCTTCGATATCACCATCGGCTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAAGTGGTGAGTGAATCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTcc                                                                                                                                                                      <  1:321145/198‑1 (MQ=255)
                                                                                                                                    aCCATCGGCTTACATGCCCATAAGTAAATTAGGCTTTGCCTCTTCGTTCTCTTTCATTGACCAAATAAAAGCACGCATCCCCGGCTTAATGGTTTTAAATAACGTGGTGAGTGAATCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGc                                                                                                                                                                                              >  1:184890/1‑160 (MQ=255)
                                                                                                                                            cTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAAGTGGTGAGTGAATCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGAt                                                                                                                                                                                           >  1:19988/1‑155 (MQ=255)
                                                                                                                                            cTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAAGTGGTGAGTGAATCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGATTTCACGCGGCATAAATTCTTTACCGGTTGGc                                                                                           >  1:17172/1‑251 (MQ=255)
                                                                                                                                               naaTGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAAGTGGTGAGTGAATCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGATTTCACGCGGCATAAATTCTTTACCGGTTGGCAGt                                                                                        >  1:91486/2‑251 (MQ=255)
                                                                                                                                                          agaaaaTTAGGCTGTGCCTCTTCGTTCTCTTTAATTGACCAAATAAAAGCACGCGTCCCCGGCTTAATGGGTTTAAATAAAGTGGTGAGTGAATCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCt                                                                                                                                                                     >  1:484126/4‑163 (MQ=255)
                                                                                                                                                                                          aaTTGAACAAATAAAAGAACGCTTCACCGGCTTAATGATTTTAAATAAAGTGGTGAGAGAATCAATCATTTGTGCTGGCGGCTCTGCGACTTCAGGTAATATCAGCGATTCACCGCCTTCACGGATTTCCTGGCTGCTCAGCGGATTTTCCTCTTCAc                                                                                                                                          >  1:486982/1‑158 (MQ=255)
                                                                                                                                                                                            ttGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAAGTGGTGAGTGAATCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAgg                                                                                                                                                                            <  1:238855/122‑1 (MQ=255)
                                                                                                                                                                                              gAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAAGTGGTGAGTGAATCAATCATTTGTGCTGGCGGCTCTGCGACTTcc                                                                                                                                                                                                          >  1:462252/1‑90 (MQ=255)
                                                                                                                                                                                               aaCAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAAGTGGTGAGTGAATCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTNAGcnn                                                                                                                                                        >  2:312984/1‑137 (MQ=255)
                                                                                                                                                                                                aCAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAAGTGGTGAGTGAATCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGATTTCACGCGGCATAAATTCTTTACCGGTTGGCAGTTTGGCATTAAGGAAGAGCGTTTCGCCAAGTGTCATCTCAAACAGCGTgn                                       <  1:312984/251‑2 (MQ=255)
                                                                                                                                                                                                  aaaTANAAGCANGNNTCACCGGCTTAATGGTTTTNAATAAAGTGGTGAGTNAATCNATCATNtg                                                                                                                                                                                                                                >  2:192004/1‑64 (MQ=255)
                                                                                                                                                                                                                    ccGGCTTAATGGTTTTAAATAAAGTGGTGAGTGAATCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCCGCGATTCACCNNCTTCCa                                                                                                                                                                              >  2:9100/1‑96 (MQ=255)
                                                                                                                                                                                                                                     aaTAAAGTGGTGAGTGAATCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGAATTCACGCGGCATAAATTCTTTACCGGTTGGCAGTTTGGCATTAAGGAAGAGCGTTTCGCCAAGTGTCATCTCAAACAGCGTGCGAACGGGCATTACGACAAATGCCTGTTCGTCTTCAAc  <  1:9100/251‑1 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     
CGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGTTCATCGCCAGGTAATGTATCGGTCGCTACACTTCCCGTCGCCTGAATAATTTCTTCGATATCACCATCGGCTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAATCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGATTTCACGCGGCATAAATTCTTTACCGGTTGGCAGTTTGGCATTAAGGAAGAGCGTTTCGCCAAGTGTCATCTCAAACAGCGTGCGAACGGGCATTACGACAAATGCCTGTTCGTCTTCAAC  >  NC_000913/2654220‑2654699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑