| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,637,791 | G→A | A366T (GCG→ACG) | creC → | sensory histidine kinase CreC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,637,791 | 0 | G | A | 96.5% | 75.9 / ‑4.0 | 29 | A366T (GCG→ACG) | creC | sensory histidine kinase CreC |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (12/16); total (12/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.32e-01 | |||||||||||
GACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAG > NC_000913/4637589‑4638023 | gACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGACGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGAtt < 1:40968/251‑1 (MQ=255) aGAAACGTTACTCCGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGAATGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCCCCGAGGTTAACGTTGCTGCTGAAACGACGTTACTGGAGCAGGAGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGGATTACGCtc > 1:324224/1‑249 (MQ=255) gACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGAATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCCTGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGACGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGCAGTGGATCAGGAacac > 1:235839/1‑251 (MQ=255) cGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAAGCACTCTGCATGGTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGTGAGATGTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGc < 1:75511/203‑1 (MQ=255) cgtcctgctgTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGGTGGCAGAAAAAAAAATCACTCGGCATGTTACTCCCACCGAGGTTAACGTTGCTGCTGAACCGACGTTACTGGAGCAGGCGCTGGGAAATTTTCTGAATATCGCAATCGATTTTTCCCGCTAGAGCgg < 1:401631/184‑1 (MQ=255) cATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGACGTTACTGGAg < 1:14813/113‑1 (MQ=255) cATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGACGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGagagn < 1:58792/164‑2 (MQ=255) cAGCGAAGCGCGCACCGTGCAGTTGGCTGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGACGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTAtttttn < 1:132426/251‑2 (MQ=255) cACCGTGCAGTTGGCAGAAAAAAAAATCACTNTGCATGTTACNCCCNNCGAGNTNANCGTTGCTGCTGAACCGACGTTACTGGAGCNGGCGCTGGGAAATTTACTGGATANNGCNNNCGNNTTTAc < 2:376547/126‑1 (MQ=255) cACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGACGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTAc > 1:376547/1‑126 (MQ=255) gTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGACGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGcc > 1:376574/1‑153 (MQ=255) gTGCAGTNGNCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGNTGCTGAACCGNCGTNNCTGGNGNANGCGCTGGGAAATTTACTGGATAACGCCATNGATTTTACCCCCGAGAGCGGTTGNNGCNNNCTNNGCGcc < 2:376574/153‑1 (MQ=255) gTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGATGAACCGACGTTACTGGAGCAGGAGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTc > 1:504876/1‑251 (MQ=255) cAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGACGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTAc > 1:30978/1‑112 (MQ=255) aCTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGACGTTACCGGAGCATGCGCTGGGAAATTTACTGTATAACGCCATCGATTTTACCCCCGAGATCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGa < 1:277501/167‑1 (MQ=255) ntctGCANNTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGACGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTAc < 2:30978/96‑1 (MQ=255) ctGCATGTTACGCCCGCCGAGGTTAACGTTGCTGGTGATCCGACCTTTCTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACGCGTCACGCTTAAGGTGCTGGATACCGGTAGTAGTATTCCTGACTACGCACTGTCACGTATTTTTGAACGCTTGTACTCTTTGCCTCGTCCAAATGGGCAAAAAagc < 1:239680/249‑1 (MQ=255) gCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGACGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACGACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAagcagc < 1:153854/250‑1 (MQ=255) tGTTACGCCCACCGAGGTTAACGTTGCTGCGGAACCGACGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCTCCGAAGTGGATCAGGAACACGTCACGCTTAAgg < 1:397716/152‑1 (MQ=255) gTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGACGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCt < 1:180467/113‑1 (MQ=255) nccGAGGTTAACGTTGCTGCTGAACCGACGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCAc > 1:297329/2‑184 (MQ=255) ggTTAACGTTGCTGCTGAACCGACGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCTGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGATCGCTTTTAcn < 1:97401/200‑2 (MQ=255) ggTTAACGTTGCTGCTGAACCGACGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGc > 1:2511/1‑206 (MQ=255) aaCGTTGCTGCTGAACCGACGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGGCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGTGTTGGCGTTCGTCAg < 1:179705/251‑1 (MQ=255) acggtgctgcTGAACCGACGTTACTGNAGCAGGNNCTNGGAAATTTACTGGATAACGNCANNNANNNTNAACCCNAGNNCGGTTGCATNACGCTAAGCGCCGAAGTgg > 2:114610/5‑108 (MQ=255) aCGTTGCTGCTGAACCGACGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTgg < 1:114610/108‑1 (MQ=255) ntgctgAACCGACGTTACTGGAGCAGGCGATGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTc > 1:404114/2‑239 (MQ=255) ctgctgAACCGACGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAgcgc > 1:21627/1‑94 (MQ=255) ctgctgAACCGACGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTa > 1:171952/1‑157 (MQ=255) ctgcnnAACCGACGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAgcgc < 2:21627/94‑1 (MQ=255) | GACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAG > NC_000913/4637589‑4638023 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |