| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,991,071 | C→T | M183I (ATG→ATA) | yecA ← | putative iron‑binding protein YecA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,991,071 | 0 | C | T | 94.7% | 39.0 / ‑3.6 | 19 | M183I (ATG→ATA) | yecA | putative iron‑binding protein YecA |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (9/9); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.72e-01 | |||||||||||
GAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCATCCAGTACGCATGTAGATCAAGCGCCGCCAGTCGAATGGCATCTACGCTCTCTTCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAATCGGGCAACGTTG > NC_000913/1990881‑1991228 | gAAGTGGGGTGATCAGCTAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGCACAGCTTTTTCCTGCGGATGCGCTATCCAGTACGCATGTAGATc > 1:110423/1‑211 (MQ=255) gCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACTCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACCGCTTTTTCCTGCGGATGCGCTATCCAGTACGCa > 1:37712/1‑184 (MQ=255) cAAATTCCTTATTGGGTGCCAGAATTAACGCTGACCCCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTCCGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCTATCCAGTACGCATGTAGATCAAGCgccgcc < 1:33871/198‑1 (MQ=255) cAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCCATTTGGCCTCTTAATGCAGGCAGCAATGCTTACATTTCTTACCAATACCGCACGGGCAAGGGTCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCTATCCAGTACGCATGTAGATCACGCGCCGCCAGTCGAATGGCATCTACGCTCTCTTCAAACGCTTCTGGCGACATCTTTTCTAc > 1:413124/1‑251 (MQ=255) aaaTTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATGTGGCCTCTTAAGGCAGGCAGCACGTCTTAAAGTTCTTACTGCTATCGCACGGGCAAGGATCGTTACGCCCCTGTTTCTCTTTTGCTTGGATCGGTTGCTGAACAGCTTTGTTCTGCGGATGCGCTTTCCAGTACGCATGTAGATCAAGCGCCGCCAGTCGAATg < 1:74723/206‑1 (MQ=255) aGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTACATTTCTTACCACTACCGCCCGGGCAAGCATCGTTACGCCACGGTTTCTCTTCTGCTTTGATCGGTTGCTGCACAGCTTTTTCCTGCGGATGCGCTATCCAGTACGCa > 1:289114/1‑160 (MQ=255) acCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCTATCCAGTACGCATGTAGATCAAgc < 1:35979/157‑1 (MQ=255) gcaggcagCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGATTTTTCCTGCGGATGCGCTATCCAGTACGCATGTAGATCAAGCGCCGCCAGTCGTATGGCATCTACGCTCTCTTCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGtttt > 1:504097/1‑251 (MQ=255) ngcagcaCTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCATCCAGTACGCATGTAGATCAAGCGCCGCCAGTCGAATGGCATCTACGCTCTCTTCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAAc > 1:147114/2‑251 (MQ=255) ggcagCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCTATCCAGTACGCATGTAGATCAAGCGCCGCCAGTCGAATGGCATCTACGCTCTCTTCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAAc > 1:138471/1‑251 (MQ=255) cTTACCACTACCGCACGGGCAAGGATCGGTACGCCCCGTTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCTATCCAGTa < 1:474451/99‑1 (MQ=255) cAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCTATCCAGTACGCATGTAGATCAAGCGCCTCCAGTCGAATg > 1:18824/1‑111 (MQ=255) cAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCTATCCAGTACGCATGTAGATCAAGCGCCGCCAGTCGAATGGCATCTACGCTCTCTTc < 1:254352/128‑1 (MQ=255) gATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGATGAACAGCTTTTTCCTGCGGATGCGCTATCCAGTACGCa > 1:172978/1‑80 (MQ=255) cGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCTATCCAGTACGCATGTAGATCAAGCGCCGCCAGTCGAATGGCATCTAcn < 1:90620/108‑2 (MQ=255) tnCTNTTCTGNTTTGNTCGGTTGCTGAACAGNTTTTTCCTGCGGATGCGCTNNCNATTACNCATGt < 2:191408/66‑1 (MQ=255) tctGCTTTGATCGGTTGCTGAACAGCTTTTTCCTTCGGAGGCGCTATCCAGTACGCATGTAGATCAAGCGCCGCCAGTCGAATGGCATCTACGCTCTCTTCAAACGCTTCTGGCGACATc < 1:469759/120‑1 (MQ=255) tgagcGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCTATCCAGTACGCATGTAGATCAAGCGCCGCCAGTCGAATGGCATCTACGCTCTCTGCAAACGCTTCTGGCGACATCGTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCan < 1:94191/147‑2 (MQ=255) gATGCGCTATCCAGTACGCATATAGATCAAGCGCCGCAAGTCGAATGGCATCTACGCTCTCTTCAAACGCTTCTGTCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAATCGGGCAACGTTg > 1:403527/1‑165 (MQ=255) | GAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCATCCAGTACGCATGTAGATCAAGCGCCGCCAGTCGAATGGCATCTACGCTCTCTTCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAATCGGGCAACGTTG > NC_000913/1990881‑1991228 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |