Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,040,126	G→T	L277L (CTG→CTT)	appB →	cytochrome bd‑II subunit 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,040,126	0	G	T	100.0%	78.8 / NA	27	L277L (CTG→CTT)	appB	cytochrome bd‑II subunit 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (10/17);  total (10/17)

CAGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTG  >  NC_000913/1039896‑1040343                                                                                                                                                                                                                                       |                                                                                                                                                                                                                          caGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTCATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGGGATGGCGATTTATCg                                                                                                                                                                                                       >  1:113542/1‑251 (MQ=255)      ggCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTgg                                                                                                                                                                                                    <  1:143047/249‑1 (MQ=255)                    cTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTAGGTATTGCTGGCTTTGTACTGCTCGCCCCGGATGCTAACGGTCCTTCCAATACGTTAATGAACCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGCGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGGGATGGCGATTTATCGTGGTCGCCCGGGTTgggg                                                                                                                                                                                     >  1:268923/1‑250 (MQ=255)                           gTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCTGGTTGGGGATTTTTg                                                                                                                                                                              <  1:388610/250‑1 (MQ=255)                                                    ncGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTCATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATTGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGt                                                                                                                                                    >  1:95501/2‑251 (MQ=255)                                                              ggCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACg                                                                                                                                          <  1:76390/251‑1 (MQ=255)                                                                                                cGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATTGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGcc                                                                                                          <  1:334489/249‑1 (MQ=255)                                                                                                     aGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCATGGGTGCATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTTCCGCTGATGGGCTTCTTCTGCCCCTTGCTTCCGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGc                                                                                                   >  1:345687/1‑251 (MQ=255)                                                                                                          gCTAACGGTCCTTCCAATCAGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTg                                                                                                >  1:137803/1‑249 (MQ=255)                                                                                                           cTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGTTCTTCCCGCTGCTGGGATGCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGAttt                                                                                                                                                                                 <  1:434886/167‑1 (MQ=255)                                                                                                                    cTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTANCGTNNNNNcc                                                                                                                                                                                               >  2:306990/1‑144 (MQ=255)                                                                                                                       ccAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCTATCTCCAgn                                                                                 <  1:150046/251‑2 (MQ=255)                                                                                                                            ncGNTAANGNNANNGGTNGCAGTGCTACCTGGTGCCTGGNNGAATAATTTNGTCGAGTCGCCCGTTTTGTTGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTNCTTACGGTGNNGGNGATTGNTNNNGNNNGCNCGGGTTGGGGATTTTTTATNGCNNCATTGANGCAATTTGGCGGGATTTTCACggcag                                                                                                                                      <  2:113542/192‑1 (MQ=255)                                                                                                                                       gcTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCa                                                                                                                                            >  1:440079/2‑176 (MQ=255)                                                                                                                                           gtggCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTAc                                                             >  1:175272/1‑251 (MQ=255)                                                                                                                                           gtggCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGAGTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGGCCGATCTCCAGCCTGACGTTGTGGGACAGTAc                                                             <  1:181878/251‑1 (MQ=255)                                                                                                                                                      aCCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGc                                                  <  1:8518/251‑1 (MQ=255)                                                                                                                                                               cTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATg                                                                                                                                                                                                                  <  1:153790/82‑1 (MQ=255)                                                                                                                                                                      aataatTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCa                                                                                                                                            <  1:164122/145‑1 (MQ=255)                                                                                                                                                                                        cccGTTTTGTGGATCGTCCCGCTGCTGGGATGCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGTTCGCCCGGTTTTGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGc                                                                                                                             <  1:382481/142‑1 (MQ=255)                                                                                                                                                                                          cGTTGTGTGGATCTTCCCGCTGCTGGGATGCTTCTGCCCAGGGCTTACGGTGGTGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATg                                                                                                                                                                           <  1:279004/94‑1 (MQ=255)                                                                                                                                                                                               tgtgGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCGGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCa                                                                                   <  1:238467/177‑1 (MQ=255)                                                                                                                                                                                                   gATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATg                                                                                                                                                                           >  1:28720/1‑85 (MQ=255)                                                                                                                                                                                                   gATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATg                                                                                                                                                                           <  2:28720/85‑1 (MQ=255)                                                                                                                                                                                                        tCCCGCTGCTGTGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCAtn   <  1:306990/248‑2 (MQ=255)                                                                                                                                                                                                        tCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTTTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCTGCCTGACGTTGTGGGATAGTTCTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATtg  <  1:275808/249‑1 (MQ=255)                                                                                                                                                                                                          ccGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGGTTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACggcaggcac                                                                                                                                  >  1:271304/1‑118 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                          CAGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTG  >  NC_000913/1039896‑1040343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑