Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,137,967	G→A	Q357* (CAG→TAG)	gldA ←	L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,137,967	0	G	A	100.0%	28.5 / NA	12	Q357* (CAG→TAG)	gldA	L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/6);  total (6/6)

GGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTG‑AGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCC  >  NC_000913/4137798‑4138181                                                                                                                                                                          |                                                                                                                                                                                                                        ggAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATCGCTGAGATTGCGTAGGCATGATAAGCGTAGCGAAGCAGGCAATTTTGCGATGAAACTAACGGACACCACGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTAGTCGGCTACCAGCAGAGCGGCGAAAACCGGATCTGGCGTCGCGCCGCCAGGGAtgttacggaagaacggaagagaacacgt                                                                                                                                        >  1:85333/1‑227 (MQ=25)                                                                     gATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGCGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTAGTCGGCTACCAGCagag                                                                                                                                                                                                        >  1:212121/1‑119 (MQ=255)                                                                                       ggCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCACACTCTTGCACGAAACGCTGACCGTACTAGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTG‑AGCGAGAGTTATTGGCAAACCTACCGCAt                                                 >  1:261106/1‑251 (MQ=255)                                                                                         cAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAAAACTGACCGTACTAGTAGGCTACCAGCAGAGCGGCGTAAAACTGATCTGGCGTCGCGCCGCCAGGCCTGTTGTGAATGGTTTCACCTTCTGCACATGACGCTTCTGc                                                                                                                            >  1:230685/1‑175 (MQ=255)                                                                                                 cGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGATGACCGTACTAGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCCTTTTCGCCGGGACATCTTCTTTAATATCCAGTTG‑AGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGc                                       >  1:223099/1‑251 (MQ=255)                                                                                                                         naNTTNGGAGTNGGTTNTTCCCACTCTTGCAGNAAACGCTGACCGTACTAGTNNGNTACCANCagag                                                                                                                                                                                                        <  2:212121/66‑1 (MQ=255)                                                                                                                                      gTTATTCCCACTCTTGCAGGAAACGCTGACCGTGCTAGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCTTCGCGCCGCCAGGCATGTTGTGAATGTTTTCACCTTCTTCGCAGGCCGGTGCTGCCACAATTCTCATGTTCGCCGTTACTGCGTCTGTAAGAGCCA‑TTGTAGCCAGAGTTAGTGGCAAACCTGCCGCTTGGCTAATAGCATCTACGGTTTCGTTTCCCGCCATCGGGGCATTTTcc  <  1:414777/251‑1 (MQ=255)                                                                                                                                        tATTCCCACTCTTGCAGGAAACGCTGACCGTACTAGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTc                                                                                                                                                                                 <  1:118493/75‑1 (MQ=255)                                                                                                                                                  ctTGCAGGAACCGCTGACCGTACTAGTCGGCTACCAGCAGAGCGGCTTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGTTTTCACCTTCTCCACATGCCGCTTCTGCCACAATTCGCATTTGCGCCGGGACATCTTCTTTAATATCCAGTTG‑AGCGAGAGTTATTGGCAAACCTGCCGc                                                   <  1:221751/190‑1 (MQ=255)                                                                                                                                                         gAAACGCTGACCGTACTAGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATcc                                                                                    <  1:194045/151‑1 (MQ=255)                                                                                                                                                             cGCTGACCGTACTAGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGc                                                                                                                                                                                    <  1:181452/51‑1 (MQ=255)                                                                                                                                                                   ccGTACTAGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGCCATCTTCTTTATTATCCAGTTG‑Ag                                                                            >  1:498289/1‑148 (MQ=255)                                                                                                                                                                          |                                                                                                                                                                                                                        GGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTG‑AGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCC  >  NC_000913/4137798‑4138181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑