| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,805,480 | C→T | G157G (GGG→GGA) | waaP ← | lipopolysaccharide core heptose (I) kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,805,480 | 0 | C | T | 94.7% | 48.6 / ‑6.2 | 19 | G157G (GGG→GGA) | waaP | lipopolysaccharide core heptose (I) kinase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (8/10); minor base A (1/0); total (9/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
AGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTGCACGTATCTGTGCCCGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGCCCAATCGGCACAATAATCTTCAAGGCTAATTGTGGGAGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCT > NC_000913/3805249‑3805723 | aGGCATCCCAAAATAAACCTTCATAAATCGCCATATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTTCACGTATCTGTGCCCGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATTCCTGCAGTATGCATTTTAc < 1:325163/251‑1 (MQ=255) atTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTGCACGTATCTGTGCCCGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATTCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGt < 1:411314/251‑1 (MQ=255) cACGTATCTGTGCCCGATGCAGATCGATAACTGCAATTTTTAATTCATCTTCCCGGCCAATAAATGGCAAATGAAGCAAACAGAGGCAAATGTAACAGTAGCGGTGATTTATTCCTGCCGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGtt > 1:430241/1‑190 (MQ=255) cAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATTCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCggg > 1:87293/1‑169 (MQ=255) naTCGATAAATGAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCACATGAAGCAAAAAGTGGAAAATGTAACAGTCGCGGTGATTTATTCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTACCACGAATATCAGGCGGGTTGACTGCCCAATCGGCACAATAATCTTCAAGGCTAATTGTGGGAGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAAt > 1:62949/2‑251 (MQ=255) tCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATTCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTgn < 1:102414/149‑2 (MQ=255) ccGGCCAGTAAATGGCAAATGACGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATTCATGACGTATGCATTTTCTGCACACTAGTTGCTACACGTGCGATCAGCATACGATTAACACGTATATCAGGCGGGTTGACTGCCCCATCGGCACAATAATCTTCACGGCTAATTGTGGGAGTGAGATATTCgg > 1:483547/1‑194 (MQ=255) ccGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATTCCTGCAGTATGCAGTTTACGCACCATAGTGGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTg < 1:460628/139‑1 (MQ=255) cAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATTCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAg > 1:442744/1‑103 (MQ=255) aaaTGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATTCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGCCCAATCGGCACAATAATCTTCAAGGCTAATTGTGGGAGTGAGATCTTCGGTAATAATAAATGAGGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAAccc < 1:186855/237‑1 (MQ=255) tGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGGCGCGGTGATTTATACCTGCAGTATGCATTTTCCGCACAATAGCTGCTCCACGTGCGATCAGCATACGCTTAACACGTATATCAGGAGGGGTGACTGCCCAATCGGCCCAATAATCTTCAAGGATAATTGTGGGAGTGCGATCTTCGGAAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTAt > 1:224337/1‑251 (MQ=255) gCAAATGAAGGAAAAAGTTGCAAATGGAACAGTCTTGGTGATGTAGTCCTGCAGTATGAATTTTACTCACCAGAGTTGCTACAGGTGCGATCAGCATACGCTTAACACGTATATCAGGCGTGtt < 1:504265/124‑1 (MQ=255) gAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATTCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCATAACACGTATATCAGGCGGGTTGACTGCCCAATCGGCACAATAATCTTCAAGGCTAATTGTGGGAGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGcc > 1:68800/1‑250 (MQ=255) aaaaGTGGCAAATGTAACAGTCGCGGTGATTTATTCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGCCCAATCGACACAATAATCTTCAAGGCTAATTGTgg < 1:400366/153‑1 (MQ=255) cgcgGTGATTTATTCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGCCCAATCGGCACAATAATCTTCAAGGCTAATTGTGGGAGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTTTATCAACGCCAACATCACTCAGGCGATGAATAGCGTgn < 1:96958/251‑2 (MQ=255) gATTTATTCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGTTCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGCCAAATCGGCACAATAATCTTCAAGGCTAATTGTGGGAGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACACTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGCTGAATAGCGTGCCActct > 1:458024/1‑251 (MQ=255) aTTTATTCCTGCAGTATGCATTTTACGCACCATAGTTGCTACGCGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGCCCAATCAGCACAATAATCTTCAAGGCCAAATGTGGGAGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATg < 1:69544/196‑1 (MQ=255) tttATTCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGCCCAATCGGCACAATAATCTTCAAGGCTAATTGTGGGAGTGAGATNTTCNNnnntannnnnn > 2:304225/1‑144 (MQ=255) tttATTCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGCCCAATCGGCACAATAATCTTCAAGGCTAATTGTGGGAGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAAcn < 1:304225/190‑2 (MQ=255) | AGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTGCACGTATCTGTGCCCGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGCCCAATCGGCACAATAATCTTCAAGGCTAATTGTGGGAGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCT > NC_000913/3805249‑3805723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |