Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,606,464 C→T A122V (GCC→GTC)  lsrF → 3‑hydroxy‑2,4‑pentadione 5‑phosphate thiolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,606,4640CT95.2% 53.1 / NA 21A122V (GCC→GTC) lsrF3‑hydroxy‑2,4‑pentadione 5‑phosphate thiolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (12/8);  minor base G (0/1);  total (12/9)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

GCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAAAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATT  >  NC_000913/1606325‑1606702
                                                                                                                                           |                                                                                                                                                                                                                                              
gCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGGCGTGGCGTTATCGAGGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCTATCAAAAAGATTATTCAGCt                                                                                                                                 <  1:425150/251‑1 (MQ=255)
               cATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGAAGCGTAGTTCCCCCTGAGACCAATAGGCAGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGTCGTGGAGTTATCGATGGATGAAGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAAAATATTATTCAGCTGGTTGATGCCGGAAt                                                                                                                  >  1:438452/1‑251 (MQ=255)
                                     ngcgcgGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGAGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGACGTCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAAAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATg                                                                                            >  1:59484/2‑251 (MQ=255)
                                           gCATTTTGCGCAGCGTAGTTCCCCCTGCGACCACTAGGCCGCTGGTACTGCGGGAGTCCGGTGCGAACTCTATTCTGGCGGAATTAAGTACTGAAGTACTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAAAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTg                                                                                      >  1:204399/1‑251 (MQ=255)
                                           gCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGTCGTGGCGTTATCGATGGCTGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAGTATGAACATCAGTCGATCAAAAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTg                                                                                      >  1:462736/1‑251 (MQ=255)
                                                    gcAGCGTAGTTCCCCCTGCGACCAATAGGCCGGAGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGTCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCACGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAAAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTg                                                                             >  1:392135/1‑251 (MQ=255)
                                                            gTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGTCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATg                                                                                                                                                                <  1:10996/160‑1 (MQ=255)
                                                            gTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGTCGTGGCGTTATCGATGGAGGACTCCGTGCGCCTGAACAGTTg                                                                                                                                                                                                      <  1:161877/122‑1 (MQ=255)
                                                                    tGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGACCTCTATTCTGGCGGAATTAAGTAATGAAGTCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTggcggc                                                                                                                                                                                          >  1:276015/1‑126 (MQ=255)
                                                                          cAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGGAGTCGTGGCGTTATCgatggatg                                                                                                                                                                                                                            >  1:260253/1‑86 (MQ=255)
                                                                               ggCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGTCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTgcg                                                                                                                                                                                                    >  1:265136/1‑105 (MQ=255)
                                                                                     tgggaCTGCGGGCGTCAGGTGCGACCTCTATTCTGGCGGAATTAAGTAATGAAGTCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAg                                                                                                                                                                                      <  1:162443/109‑1 (MQ=255)
                                                                                       ntACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGCATTAAGTAATGAAGTCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATc                                                                                                                                                  >  1:57047/2‑147 (MQ=255)
                                                                                         aCTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGTCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAAAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTctcg                                         <  1:41184/250‑1 (MQ=255)
                                                                                          cTGCGGGCGTCAGGTCCGAACTATATTATGGCGGAATTAAGTAATGAAGTCGTGGCGTTATCGATGGATGACGCAGCGCGCCTGAACAGTTGCGCGGTg                                                                                                                                                                                               >  1:419249/1‑99 (MQ=255)
                                                                                             cGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGTCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAAAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGa                                                                                                 <  1:271911/190‑1 (MQ=255)
                                                                                             cGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGTCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAAAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTctcgn                                        <  1:126946/247‑2 (MQ=255)
                                                                                                  gTCAGNTGCGCNCNNTATTCTGGCGGAATTAAGTNATGAAGTCGTGGCGTNATCGNTGGATNatnc                                                                                                                                                                                                                        >  2:199355/1‑63 (MQ=255)
                                                                                                  gTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGTCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAAAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTa                                                <  1:199355/234‑1 (MQ=255)
                                                                                                                                aaGTAATGAAGTCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAAAATATTATTCAGATGGTTGTTGCCGGCATGAAAGTGGGAAAGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAAtt  >  1:273499/1‑250 (MQ=255)
                                                                                                                                    aaTGAAGTCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAAAATATTATTCAGCTggn                                                                                                                              <  1:100651/122‑2 (MQ=255)
                                                                                                                                           |                                                                                                                                                                                                                                              
GCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAAAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATT  >  NC_000913/1606325‑1606702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑