| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,374,201 | G→A | G38S (GGC→AGC) | ycjP → | putative ABC transporter membrane subunit YcjP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,374,201 | 0 | G | A | 96.2% | 67.3 / ‑3.3 | 26 | G38S (GGC→AGC) | ycjP | putative ABC transporter membrane subunit YcjP |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (10/15); total (11/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.96e-01 | |||||||||||
CGGTGTATATCTCTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACAAATAAACGCACACTCAGTCGCATCGGTTTTTACTGCGGGCTGGCGCTGTTTCTCATCATCAC‑GCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCGTAACAGTCTGGTGGTGTCTGTGGTTTCATCCGTGGTGGCGGTATTCCTCGGCATTCTTGGGGCTTACGCGCTTTCCCG > NC_000913/1373965‑1374391 | cGGTGTATATCTCTACAAATCCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACAAATAAACGCACACTCAGGCGCATCGGTTTGGACTTCGGGCTGGCGCTGTGTCTCATCATCAC‑GCTGTTTCCATTGTTTGTGATGCTGATGACCTCGTTCAAGAGCGCGAAAGAGGc < 1:371744/250‑1 (MQ=255) gtATATCTCTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAAAAAATAAACGCACACTCAGTCGCATCGGTTTTTACTGCGGGCTGGCGCTGTTTCTCATCATCAC‑GCTGTTTCTATTTTTTGTGATGCTGATGACCTCGTTCAAGAGCGCTAAAGAGTCGAtc < 1:412161/250‑1 (MQ=255) aTGATTGAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACAAATAAACGCACACTCAGTCGCATCGGTTTTTACTGCGGGCTGTCTCTGTTTCTCATCATCAC‑GCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGAGCGCGAAAGAGTCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTg < 1:337082/251‑1 (MQ=255) gtCTGCTGACCAGTAAACGGGTGAACCTCAATGGCAACAAATAAACGCACACTCAGTCGCATCGGTTTTTACGGCGTGCTTGCGCTGTTTCTCATCATCAC‑GCTTTTTCCATTTTTTGTGCTGCTTATGTCCTCGTTCAAGAGCGCGAAAGAGGc < 1:442700/154‑1 (MQ=255) ccAGGAAACGGGTGAACCTCAATGGCAACAAATAAACGCACACTCAGTCGCATCGGTTTTTACTGCGGGCTGGCGCTGTTTCTCATCATCAC‑GCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGAGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTcc < 1:396429/244‑1 (MQ=255) ccAGGAAACGGGTGAACCTCAATGGCAACAAATAAACGCACACTCAGTCGCATCGGTTTTTACTGCGGGCTGGCGCTGTTTCTCATCATCAC‑GCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGAGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCGTAAc > 1:355474/1‑249 (MQ=255) naaaCGGGTGAACCTCAATGGCAACAAATAAACGCACACTCAGTCGCATCGGTTTTTACTGCGGGCTGGCGCTGTTTCTCATCATCAC‑GCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGAGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGAttt > 1:150708/2‑219 (MQ=255) ccTCAATGGCAACAAATAAACGCACACTCAGTCGCATCGGTTTTTACTGCGGGCTGGCGCTGTTTCTCATCATCAC‑GCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGAGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCGTAACAGTCTGGTGGTGTCtgtg > 1:379276/1‑251 (MQ=255) ccTCAATGGCAACAAATAAACGCACACTCAGTCGCATCGGTTTTTACTGCGGGCTGGCGCTGTTTCTCATCATCAC‑GCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGAGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCGTAACAGTCTGGTGGTGTCtgtg < 1:432540/251‑1 (MQ=255) aTGGCAACAAATAAACGCACACTCAGTCGCATCGGTTTTTACTGCGGGCTGGCGCTGTTTCTCATCATCAC‑GCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGAGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCGTAACAGTCTGGTGGTGTCTGTGGTTTc > 1:454638/1‑251 (MQ=255) caacaaATAAACGCACACTCAGTCGCATCGGTTTTTACTGCGGGCTGGCGCTGTTTCTCATCATCAC‑GCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGAGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTAc > 1:409815/1‑173 (MQ=255) aaCGCACACTCAGTCGCATCGGTTTTTACTGCGGGCTGGCGCTGTTTCTCATCATCAC‑GCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGAGCGCGAAAGAGGCGATCTCACTGAATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCGTAACAGTCTGGTGGTGTCTGTGGTTTCATCCGTGGTGGCg > 1:30405/1‑251 (MQ=255) cGCACACTCAGTCGCATCGGTTTTTACTGCGGGCTGGCGCTGTTTCTCATCAT‑ACAGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGAGCGCGACAGTGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGCCGCTGGAGCATTACGTCGACATTTTTAACCCgatgc > 1:382583/1‑183 (MQ=255) gCACACTCAGTCGCATCGGTTTTTACTGCGGGCTGGCGCTGTTTCTCATCATCAC‑GCTGTTTCCATTTTTTGGGATGCTGATGACCTCGTTCAAGAGCGCGAAAGAGGCGATCTCACTGCATCCTACgc < 1:86291/129‑1 (MQ=255) cacTCAGTCGCATCGGTTTTTACTGCGGGCTGGCGCTGTTTCTCATCATCCC‑GCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCAc > 1:485007/1‑114 (MQ=255) tCAGTAGCATCGGTTTTTACTGCGGGTTGGCGCTGTTTCACATCATCAC‑GCTGTTTCCCTTTTTTGTGATGGTGATGACCTAGTTACAGAGCGCGAAAGAGGAGATATCACTGAATCCTACGCTGCTTCCGCAGCAGTGGACGCTGGAGCCTTACGTCGACATTTTTAACCCGATGATTTTCCAGTTTGTCGCCTACTTCCGTAACAGTCTGGTGGTGTCTGTGGTTTCATCCGTGGTGGCGGTATTCCTc > 1:454631/1‑251 (MQ=255) tttACTGCGGGCTGGCGCTGTTTCTCATCATCAC‑GCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGAGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCGTGACAGTCTGGTGGTGTCTGTGGTTTCATCCGTGGTGGCGGTATTCCTCGGCATTCTTggggn < 1:319720/250‑2 (MQ=255) ttACTGCGGGCTGGCGCTGTTTCTCATCATCAC‑GCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGAGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCgcagc < 1:338001/119‑1 (MQ=255) cTGTTTCTNANNNTNNC‑GCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGAGCNCGAAAGAGGCGATCTNACNGCATNCTANgc > 2:349176/1‑91 (MQ=255) cTGTTTCTCATCATCAC‑GCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGAGCGCGAAAGAGGCGATCTCACTGCATCCTACgc < 1:349176/91‑1 (MQ=255) cTGTTTCTCATCATCAC‑GCTGTTTCCATTTTTTGTAATGCTGATGACCTCGTTCAAGAGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCGTAACAGTCTGGTGGTGTCTGGAGTTTCATCCGTGGTGGCGGTATTCCTCGGCATTCTTggg < 1:365733/231‑1 (MQ=255) c‑GCTGTTTCCATGTTTTGTGATGCTGATGGCCTCGGTCAAGAGCGCGAAAGAGGTGATCTCTCTGCTGCCCTCTCGGCGGCCGCAGCAGTTTACTCTGTAGCATTACGTCGACATTTTTAACCCgatga < 1:451994/129‑1 (MQ=255) nnngtgNTGCTGATGACCTCGTTCAAGAGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCg < 2:309062/137‑1 (MQ=255) aTGCTGATGACCTCGTTCAAGAGCGCGAAAGAGGCGATCTCACTTCATCCTACGCTGCTGCCGCAGCAGTGGCCGCTGGAGCATTACGTCGACATGTTTAACCCGATGATTGTCCCTTTTGTCGACTACTTCCGTAACAGTCTGGTGGTGTCTGTGGTTTCATCCGTGGTGGCGTTATTCCTCGTCATTCTTGGGACTTACGCGCTTTccct < 1:165901/212‑2 (MQ=255) aCCTCGTTCAAGAGCGCGAAAGAGGCNATCTCANNGCNTCCTACGCTGCTGCCGCAGNAGNNNANNNTNAAGCANTANNTCGACATTTNTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCGTAACAGTCTGGTGGTGTNTGTGGTTTCNNCCGTGGTGGCgg > 2:116027/1‑166 (MQ=255) aCCTCGTTCAAGAGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCGTAACAGTCTGGTGGTGTCTGTGGTTTCATCCGTGGTGGCgg < 1:116027/166‑1 (MQ=255) | CGGTGTATATCTCTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACAAATAAACGCACACTCAGTCGCATCGGTTTTTACTGCGGGCTGGCGCTGTTTCTCATCATCAC‑GCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCGTAACAGTCTGGTGGTGTCTGTGGTTTCATCCGTGGTGGCGGTATTCCTCGGCATTCTTGGGGCTTACGCGCTTTCCCG > NC_000913/1373965‑1374391 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |