| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,718,545 | G→A | intergenic (‑17/‑190) | yfiF ← / → trxC | putative methyltransferase YfiF/reduced thioredoxin 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,718,545 | 0 | G | A | 95.7% | 58.4 / NA | 23 | intergenic (‑17/‑190) | yfiF/trxC | putative methyltransferase YfiF/reduced thioredoxin 2 |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (14/8); minor base C (1/0); total (15/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAGAAACATACCGGCGTCGTTGCCGATAAGTCTCCTTACTCATCCCGAGGTTAGTT > NC_000913/2718335‑2718734 | tCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCACTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCgg < 1:113145/248‑1 (MQ=255) gTCACGGGACTGCCTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTCTCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCACTGCGCGAAGCGCGCAGATTACCCGagatcggaagagccaccgtctgaact > 1:72648/1‑226 (MQ=255) cGGGGCTGACTCTGTTTGTAATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCACTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTAc > 1:420705/1‑251 (MQ=255) gcgggcggGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCACTGCGCGAAgc < 1:258495/184‑1 (MQ=255) gcggggcgACGGCCAACGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCACTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTg > 1:411387/1‑251 (MQ=255) nggcgACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCACTGCGCGAAgcgcg > 1:127638/2‑180 (MQ=255) ggcgACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCACTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAAt > 1:116995/1‑250 (MQ=255) gcgACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCACTGCGCGa > 1:78862/1‑172 (MQ=255) ccACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTCCGTTTATCAGAATCATCATCACTGCGGCCATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCCCCAGCACTGCGCGAAGCGCGCAGATTAcc > 1:444401/1‑180 (MQ=255) ccACCGTCAGCACGAGATTTTACTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTCTCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTAATGCTTTTCTCCACCAGCCCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAAc > 1:327596/1‑247 (MQ=255) cgcccCCTTTCCCGGAACGCGGGTTGTGGGTACGTTTATCCGAATCATCATCCCTGCAGCCATCCCTCCCTTTCACCTTGCCGCTTTTACCTTTCATTTCCTCGTTCATGCTTTTCTCCACCAGCACTCCGCGAAGCGCGCAGATTACTCGACGTCCGCGCGGTTCGCCATggtt > 1:491094/1‑172 (MQ=255) ggTACGTTTATCAGCATCATCATCACNGCGGACNNACNTCACTTTGACCTTGCCGCTNTTNNNTNNNANTTCATNGTNNATGCTTTTCNCCACCAGCACTGCGCGCAGCGCGCAGATTAccc > 2:118483/1‑122 (MQ=255) ggTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCACTTCGCGAAGCGCGCAGATTAccc < 1:118483/122‑1 (MQ=255) tCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCCTTTCATCGTTCATGCTTTTCTCCACCAGCACTGCGCGAAGCGCGCAGATTACCCGAAg > 1:200487/1‑116 (MQ=255) nctncaCNGNNGNNATANATCACGTTGACCTTGCCGCTTNNACCTTGCATNGCATCGTTCATGCTTTTCTCCACCAGCACTGCGCGAAGCGCGCAGATTACCCGNAGTCCGCGCNNTTNGTCATNANNNCNNNCCNAAGCCTGCGACTATCATACNTANNGAATAANACAGATTGTTGTCTGGAACAATGTcc < 2:116995/189‑1 (MQ=255) catCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCACTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTAACAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAGAAACATACCGGCGTCGTTGCCGATAAGTCTC‑ATACTCATCCCGAGgttagtt > 1:72441/1‑250 (MQ=255) cTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCACTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAGAAACATACCGGCGTCGTTGCCGATAAGTCTCCTTAc > 1:19008/1‑229 (MQ=255) ccTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCACTGCGCGa > 2:43269/1‑59 (MQ=255) ccTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCACTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGAttg < 1:43269/147‑1 (MQ=255) tGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCACTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGGTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATtgttgt > 1:22838/1‑148 (MQ=255) nnnnttACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCACTGCGCNAAGCGNGNANNTTNNCCGAAGTc < 2:338944/70‑1 (MQ=255) ntcattncatCNTTCATGCTTTTCTCCNCCAGCACTGCGCGAAGCGCNNANATTACNCGAAg < 2:200487/61‑1 (MQ=255) ccaccaGCACTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAGAAAc < 1:155854/149‑1 (MQ=255) | TCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAGAAACATACCGGCGTCGTTGCCGATAAGTCTCCTTACTCATCCCGAGGTTAGTT > NC_000913/2718335‑2718734 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |