| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 897,642 | G→A | V187I (GTC→ATC) | potI → | putrescine ABC transporter membrane subunit PotI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 897,642 | 0 | G | A | 94.1% | 40.9 / NA | 17 | V187I (GTC→ATC) | potI | putrescine ABC transporter membrane subunit PotI |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (5/11); minor base C (1/0); total (6/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
CAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGTCATTACGCTACCGATGATCATGCCCGCGATCATTTCTGGCTGGTTACTGGCTTTTACTTTGTCGCTTGATGATCTGGTGATCGCCAGCTTTGTTTCCGGGCCGGGAGCCACCACGTTACCGATGCTGGTCTTTTCCAGCGTGCGGATGGGGGTGAATCCGGAAATCAACGCCCTGGCAACATTGATCCTCGGCGCGGTCGGAATTGTCGGA > NC_000913/897421‑897854 | cAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCAGTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGAGTGCGGGAACGGGAGCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCATCATTACGCTACCGATGATCATGCCcgcg < 1:442650/251‑1 (MQ=255) cGCTTTTGTAATTATTCGTCGCACTTGCTCATGCCATTGTCTGGCGTGCGGACAGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTTAAGATGGCGGGAACTGGTTCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGGGACGGCGCTGAAAGTGTTTTGCATCATTACGCTTCCGATGATCATGCCCGCGATCATTTCTGGCTGGTTAcc < 1:351777/250‑2 (MQ=255) cGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATTTGGCGGTCGTTCTTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCATCGTTACGCTACCGATGACCATGCCCGCGATCATTTCTGGCTGGTTACtt > 1:486602/1‑250 (MQ=255) gCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTGTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCATCATTACGCTACCgatgatn < 1:57935/220‑2 (MQ=255) ccTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTAACTTTTTGTACGGCTTCTGTGGCGGTTGTTATTTCTTCCAGATTGCGGGAAACGGATCGCTCGCTAGAAGAAGCAGCGATGGGTCTCGGTGCGAAGCCGCTGAAAGTGTTTTTCATCATTACGCTACCGATGATCATGCCCGCGATCCTTTCTGGCTGGTTACTGGCTTTTACTTTGTCGCTTGATGATCTGGGGCTCGCCAGCTTTg > 1:248858/1‑250 (MQ=255) gACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCATCATTACGCTACCGATGATCATGCCCGCGATCATTTCTGGCTGGTTACTGGCTTTTACTTTGTCGCTTGATGATCTGGTGATCGCCAGCTTTGTTTccgg > 1:212966/1‑251 (MQ=255) aCCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGTAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTGCATCAGTACGCTACCGATGATCATGCCCGCGATCATTTCGGGCTGGTTACTGGCTTTTACTTTGTCGCTTGATGATCTGGTGATCGCCAGCTTTGTTTccggn < 1:59347/251‑2 (MQ=255) gcgGTATGCTCACCAGCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGGCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCATCATTACGCTACCGATGATCATGCCCGCGATCATTTCTGGCTGGTTACTGGCTTTTACTTTGTCGCTTGATGATCTGGTGATCGCCAg < 1:268804/235‑1 (MQ=255) ggctggcGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTAAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGAAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCATCATTACGCTACCGATGATCATGCCCGCGATCATTTCTGGCTGGt > 1:435961/1‑174 (MQ=255) cgcATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATGTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCATCATTACGCTACCGATGATCATGCCCGCGATCATTTCTGGCTGGTTACTGGCTTTTACTTTGTCGCTTGATGATCTGGTGATCGCCAGCTTTGTTTCCGGGCCGGGAGCCACCACGTTACCGATGCTg < 1:953/251‑1 (MQ=255) gcctGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATGTCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGGTTTTCATCATTACGCTTCCGATGATCATGCCCGCGATCATTTCTGGCTGGTTACTGGCTTTTACTTTGTCGCTTGATGATCTGTTGATCGCCAGCTTTGTTTCCGGGCCGTGAGCCACCACGTTACCGAGGCTga < 1:385644/248‑2 (MQ=255) ttGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGAGGGATCTCGGTGCGACGCCGCTGAAAGTTTTTTTCATCATTACGCTACCTATGATCATGCCCGCGATCATTTCTGGCTGGTTACTGGCTTTTACTTTGTCGCTTGATGATCTGGGGATCGCCAGCTTTGTTTCCTGGCCGGGAGCCACCACGTTACCGATGCTTGTCTTTTcc < 1:83675/247‑1 (MQ=255) ggATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGAGATTTTCCTCATTAAGCTACCGATGATCATGCCCGCGATAATTGCTGGCTGGTTACTGGCTTTTACTTTGTCGCTTGATGATCTGGCGATCGCCTGCTTTGTTTCCGGGCCGGTAGCCACCACGTTCCCGCTGCTGGTCTTTTCCAGCGTGCGGATGGGGGTGAATCGGGAAATCAACGACCTGGAAACATTGATcc > 1:495846/1‑251 (MQ=255) gATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCATCATTACGCTACCGATGATCATGCCCGCGATCATTTCTGGCTGGTTACTGGCTTTTACTTTGTCGCTTGATGATCTGGTGATCGCCAGCTTTGTTTCCGGGCCGGGAGCCACCACGTTACCGATGCTGGTCTTTTCCAGCTTGCGGATGGGGGTGAATCCGGAAATCAACGCCCTGGCAACATTGATCCTCGGCGCgg < 1:236031/251‑1 (MQ=255) ggATGGATCTCGGTTCGACGCCGCTGAAAGTGTTTTTCATCATTACGCTACCGATGATCATGCCCGCTATCATTTCTGGCTGGTTACGGGCTTTTACTTTGTCGCTTGATGATCTGGTGATCGCCAGCTTTGTTTCCGGTCCGGGAGCCACCACGTTACCGATGCTGGTCTTGTCCAGCGTGCGGATGTGGGTGAATCCGGAAATCAACGCCCTGGCAACATTGATCCTCGGCGCGGTCGGAATTGTCGGa < 1:71670/250‑1 (MQ=255) cGACGCCGCTGAAAGTGGTTTTCATCATTACGCTACCGATGATCATGCCCGCGATCATTTCTGGCTGGTTACTGGCTTTTACTTTGTCGCTTGATGATCTGGTGATCGCCAGCTTTGTTTCCGGGCCGGGAGCCACCACGTTACCGATGCTGGTCTTTTCCAGCGTGCGGATggggg > 1:403069/1‑177 (MQ=255) ttttCATCATTCCGCTACCGATGATCATGCCCGCGATCATTTCTGGCTGGTTACTGGCTTTTACTTTGTCGCTTGATGATCTGGTGATCGCCAGCTTTGTTTCCGGGCCGGGAGCCACCACGTTACCGATGCTGGTCTTTTCCAGCGTGCGGATGGGGGTGAATCCGGAAATCaan < 1:128422/176‑2 (MQ=255) | CAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGTCATTACGCTACCGATGATCATGCCCGCGATCATTTCTGGCTGGTTACTGGCTTTTACTTTGTCGCTTGATGATCTGGTGATCGCCAGCTTTGTTTCCGGGCCGGGAGCCACCACGTTACCGATGCTGGTCTTTTCCAGCGTGCGGATGGGGGTGAATCCGGAAATCAACGCCCTGGCAACATTGATCCTCGGCGCGGTCGGAATTGTCGGA > NC_000913/897421‑897854 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |