| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,965,392 | C→T | R80H (CGC→CAC) | rhlB ← | ATP‑dependent RNA helicase RhlB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,965,392 | 0 | C | T | 88.2% | 38.2 / NA | 18 | R80H (CGC→CAC) | rhlB | ATP‑dependent RNA helicase RhlB |
| Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (7/8); minor base G (0/2); total (8/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGT > NC_000913/3965154‑3965619 | aaTGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTGCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGTGATCGGCAATCg < 1:411201/251‑1 (MQ=255) gCCAATCAGAATGTCAACGCCGCTTTCCAGCACGTTCAGCTGTTTGGCGTAGCCATCACCACCGGAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGACAGCGGTTATGCGTCGGCATGGATATGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGTGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTccc < 1:3605/249‑1 (MQ=255) ccAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCCCCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCGGGGGTTCGGCGGCGGCATGGATCTGCACGGCAAGTTCACGCGTGGGTGCCATAATTAAGGCACGCTGCTGATTCACCTTGGGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAATAAACGc < 1:79614/236‑1 (MQ=255) aGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGACAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGTGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGc > 1:211359/1‑251 (MQ=255) cAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGTGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGAc < 1:12939/203‑1 (MQ=255) cAGGCCAGCAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGTGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTAt > 1:121186/1‑251 (MQ=255) ggCCAGTAGCTGCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATGCACCTTGTGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGc < 1:195626/185‑1 (MQ=255) cAGTAGCTTCCGCCAGCGGTTCTGGGTCGGCATGGATCTGGACGGCATGTTCACGCGTCGGTGCCATAATTAAGGTACGCGGCTGATTCACCTGGGGATCGGCAATCGCAGGATGAGAAAGAAGATAATGAAACTTTGACTTAAGAAGCCCCATGGTTTGCCCGGGACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAAc < 1:196548/251‑1 (MQ=255) gCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGTGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAAcn < 1:304923/213‑2 (MQ=255) gCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGTGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTNTGCNNNNNccnnnnnnnnnn > 2:304923/1‑144 (MQ=255) ccATAATTAAGGCACGCGGCTGATTCACCTTGTGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTg > 1:120196/1‑162 (MQ=255) aGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGCAGATAATGAAACGCTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGACGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAAtgt > 1:220392/1‑251 (MQ=255) gcgGCTGCTTCACCTTGTGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAACCGTTATCGTAAGCAACGCCATCGTTTTCCCGGTCCCGGTTTGCGCCTGCCCGGCTACGTccc > 1:359042/1‑115 (MQ=255) gcgGCTGATTCACCTTGTGATCGGCAATNGCANGATGNGANAGAAGATAATGAAACNTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCNNCNNNGNTGCGTCAc < 2:359042/117‑1 (MQ=255) cgGCTGAATCACCTTGTGATCGGCAAGCGCAGGATGAGAGAGAAGATAATGAAACGTTGAGGGAAGAAACGCCATCGTTGTCCCGGTTCCGGTTGTCGCCTGCCCGGCTACGTCGCGCCCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATggg < 1:493290/156‑1 (MQ=255) cTGATTCACCTTGTGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAAc > 1:160634/1‑223 (MQ=255) cACCTTGTGATCGGCAATCGCAGGATGAGAGAGAAGATAATGCAACGTTGCCGTAAGAAAAGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGt > 1:69966/1‑136 (MQ=255) aCCTTGTGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTgcg < 1:177252/88‑1 (MQ=255) | AATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGT > NC_000913/3965154‑3965619 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |