| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,725,176 | T→G | E48A (GAG→GCG) | glyQ ← | glycine‑‑tRNA ligase subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,725,176 | 0 | T | G | 95.8% | 65.2 / NA | 24 | E48A (GAG→GCG) | glyQ | glycine‑‑tRNA ligase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (13/10); total (13/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGC > NC_000913/3725020‑3725412 | ntgnggtntnncnncttnttatTGTCCGGCGATGGCTTANNGACCTCCTGNAACGTATAGGAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGGNGGGCGGTGANNAGNCGGAANANNNGNNNCCNCCATAGGTTCTGGCCCCAGNGCNNGCAGACNGGTCATTGGGTGAGAGGTTCCCgcgc < 2:115289/174‑1 (MQ=255) gCTCCTGAATATTGTCCGGCGATGGCTTAATGGCCACCTGGAACTGATAGTCGTGCGATAAACGCTTGGGGTTTTCGGCGTTGCGACGATCGCTCGGGCGAAGAGAAGGCTGTACATAAGCTGCAGCGATCGGTTCTGGCGCCAGCGAGCGCAGACAGGTCATTGGGAGCGAGGTTCCCGCGCCGAc > 1:288962/1‑187 (MQ=255) cTGAATGTTGTCCGGCAATGGCTGAATGCCCACCTGAAACTGATATTAGTGATGTAAACGGTAGGGGTTTTCACCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCAATCGGTTCTGGCCCCAGAGCGCGCAGACAGGTCATTGGGTGAGAAGATCCCGCGCCGACTTACATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCACGTAATCCTGTAAGGTCAGGATc > 1:429412/1‑250 (MQ=255) accTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTg < 1:177057/251‑1 (MQ=255) ggAACTGATAGTAGTGATGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTTGAAGTTCCTGGTATCAAACTTTTGCa < 1:137847/249‑1 (MQ=255) cTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCACCCGCCATCGGTTCTGGCCCCCGCGCGCGCAGACAGGCCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGAt > 1:290457/1‑251 (MQ=255) cGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCTAGCCCAGTAATCCTGGAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGGATCAAACTTGTGCATATTATTTCGTGCTGGATACGtgtg < 1:343123/251‑1 (MQ=255) ggggTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATAGGTTCTGGCCCAAGAGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATAAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGAttt > 1:367102/1‑251 (MQ=255) gggTTTTCGCCGTAGCGACCATCGGACGGGCGCCGAGAAGGCTGAACATAAGCAGCCGCCCTCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTg > 1:157461/1‑194 (MQ=255) gTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATATGCAGCCGCCATCGGTTCTGCCCCCAGCGCACGCAGACAGGTCATTGGGTGAGAGGTTCCAGCGATGACTTCTATGTCCACAGGTTGAACAATGGTGCAGCCATGGCGAGCCCAGTAATCCTGTAAGGTCCGGATCAAGCCCTGGACGGTCCTGGTCTCAAACTTTTGCATATTATTTCGt > 1:341059/1‑220 (MQ=255) gCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAggg < 1:365860/246‑1 (MQ=255) ggtcggGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCCGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGc > 1:158524/1‑251 (MQ=255) ggtcggGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCaa > 1:169172/1‑165 (MQ=255) ngacgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCtggatgg > 1:317079/2‑251 (MQ=255) gacgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGATAGGTTCCCGCGCCGACGTCCATg < 1:420869/95‑1 (MQ=255) aGGCTGAACATAAGCAGCCGCCATCGGTTNTGGCCNCNGNNCGNNCAGACAgg < 2:322298/53‑1 (MQ=255) aGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAgg > 1:322298/1‑53 (MQ=255) ncNGANNATAANCAGCCGCCATCGGTTCTGGCCCCANCGCGCGCAGACAGGTCATTGGGTGAGAGGTTNCNGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGANCCCAGTAATCNTGTNNGGTCNGNANCAAGCCCTGGAAGGTCCTGGTATCAAACTNTTGCATATTATTTCGTGCTGGATNNGTNNNGANNTAaag < 2:367102/213‑1 (MQ=255) cTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCAtn < 1:300250/85‑2 (MQ=255) cTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATg > 2:300250/1‑85 (MQ=255) aaCATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAAGTGCCCGCGCCGACTTACATGTCCAATggg < 1:144617/91‑2 (MQ=255) ccgccATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCgcgc > 1:272171/1‑60 (MQ=255) tCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATg < 1:29590/83‑1 (MQ=255) tCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATg > 2:29590/1‑83 (MQ=255) gTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGc < 1:71325/251‑1 (MQ=255) | CCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGC > NC_000913/3725020‑3725412 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |