| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 378,493 | C→T | G360D (GGC→GAC) | frmA ← | S‑(hydroxymethyl)glutathione dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 378,493 | 0 | C | T | 95.2% | 54.6 / NA | 22 | G360D (GGC→GAC) | frmA | S‑(hydroxymethyl)glutathione dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (12/8); minor base G (0/1); total (13/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
GTGGGAGATAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATGGTATGCGTGACAAACGGTTCCAGATCGATATCACCTTTCATCGCATCTTCAACCATGCCCGGTAACTGGGAACGACCTTTCACGCCGCCAAACGCGGAACCTTTCCATACGCGACCAGTGACCAACTGGAATGGACGGGTGGAGATTTCCTGACCGGC > NC_000913/378263‑378698 | gtggGAGATAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGTCTTCATGCATCAGGTCGAAg < 1:30377/251‑1 (MQ=255) cATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCCGGGAAAAACCCACGGAAGGCTGACAGGGGAGAGTCTTACGCACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGTATTCATGCATCAGGTCGAAGGCGTCATTAATTTCATACAGGCTCATGGTATGCGTGACAAACGGTTCCAGATCGatat > 1:227971/1‑251 (MQ=255) cAGCCGCCAGAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCGCAGTGTTAATCCCCCGGACGGCTTCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGTGGTAAACCTGCTGAAAATCAGGAACGAATTACGGTTCGAATGGATTTGTCGTCATGCATCAGGTCTAAGGCTTCATTAATTTCATCCAGGCTCATGGTATGCGTGan < 1:58674/221‑2 (MQ=255) gccAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTCATCCACCGGACGGCTGCCCGGGGAGAGTATTAAGAAACCCCGCCTGCAGTGGACGGGGTAAACCTGCGGGCCCTCAGTAACGAATTACGGTTCGAATGGATTTGTCTTCATGCATCCGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATGGTCTGCGTGAc > 1:329261/1‑216 (MQ=255) aCATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGTCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATGGTATGCGTGACAAACGGTTCCAGATCGATATCACCTTTCATCGCATCTTCAAcaat > 1:4209/1‑248 (MQ=255) cccGGNNGGNNNCCNNGGGAGAGTATTAAGAACCCCCGCNTGAAGTGGACGGGGTAAACCTGCGGGACANCNGNAACGNNTTANGGTTCGAATGNATTTGTCTTCATGCATCAGGTCGACGGCGTCATTACTTTCATCCAGGCNCNTGGTATGCGTGCCAAACGGTTCCAGATCGATNTCACCTTTCATCGCATCTTCACCCNAGCCNNGTnan > 2:375683/1‑213 (MQ=255) cccGGACGGCTGCCCGGGGAGATTATTAAGAACCCCCGCCTGAAGTTGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCTAATGGATGTGTCTTCATTCATCAGTTCGAAGGCGTCATTAATTTCATCCAGGCTCATGGTATGCGTGTCAAACGGTGCCAGATCGATATCACCTTTCATCTCATCTTCAACCATGCCCGGTAACTGGGAACGACCTTTc < 1:375683/229‑1 (MQ=255) ggCTGCCCGGGGAGAGTATTAAGAACCCTCGCCTGAAGTGGACGGGGTAATCCGGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGTCTTCATGCATccgg < 1:442749/108‑4 (MQ=255) cTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGAACGGGGTAAACCTGCGGGAACTCAGTTACGTATTCCGGCTAGTATGGATTTGTCTTCATGAATCAGGTCGAAGGAGTCATTAATTTCATCCAgg > 1:451508/1‑133 (MQ=255) gCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAACTCAGTACCGAATTACGGTTCGAATGGATTTGTCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCCTGGTATGCGTGACAAACGGTTCCAGATCGATATCACCTTTCATCGCATCTTCAACCATGCCCGGTAACTGGGAAc > 1:466079/1‑210 (MQ=255) gagaGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGTGGGAAAGGAGGAACTAATAACGGTTCGAATGGATTTGTCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGc < 1:497078/125‑1 (MQ=255) gagaGTATTAAGAACCCCCGCCTGAAGTGGACGGGGGAAGCCTGCGGGAAATCAGTTACGAATTACGGTTCGAATGGATTTGGCTTCATGCATCAGGTCGAATGCGTCATTAATTTCATCCAGGCTCATGGTATGCGTGACAAACGGTTCCAGATCGATATCACCTTTCATc < 1:321937/172‑1 (MQ=255) aGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGTCTTCCTGCATCAGGTCGAAGGCGTCATTACTTTCATCCAGGCTCATGGTATGCGTGACAAACGGTTCCAGATCGATATCACCTTTCATCGCATCTTCAACCATGCCCGGTAACTGGGAACGACCTTTCACGCCGCCAAACGCGGAACCTTTCCATACGCGACCAGTGACCAACTGGa > 1:193381/1‑250 (MQ=255) gACGGNNTANNNCTNNGGGAAATCAGTAACGAATTACGGNTCGAATGGATTTGTCTTCATGCATCAGGtn > 2:369150/1‑69 (MQ=255) cGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGTCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATGGTATGCGTGACAAACGGTTCCAGATCGATATCACCTTTCATCGCATCTTCAACCATGCCCGGTAACTGGGAACGACCTTTc > 1:384090/1‑180 (MQ=255) gTAAACCTNCNNNANNTCAGTAACGAATTACGGTTCGAATGGATTTGTCTTCATGCATCANGTCGAAGGCGTCATTNATNTCATNCAGNCTCATGGTATGCGTGACAAACGGTTCCAGATCGATATCACCTTTCATCGCAt > 2:346762/1‑141 (MQ=255) gTAAACCTGCTGGAAATCAGTAACTAATTTCGGGTCGAATGGTTTTTTCGGCATGCAGCAGGTCTAAGGCGTCAGTAATTTCATCCAGGCTCATGGTATGGGTGACAAACGGTTCCAGATCGATATCACCTTTCATCGCAt < 1:346762/141‑1 (MQ=255) cGGGAAATCAGCAACGAATTACGGTTCGAATGGATTTGACTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCt > 1:169922/1‑82 (MQ=255) gAAATCAGTAACGAATTACGGTTCGAATGGATTTGTCTTCATGCATCAGGTCGAGGGCGTCCTTAATTTCATCCAGGCTCATGGTATGCGTGACAAACGGTTCCAGATCGATATCACCTTTCATCGCATCTTCAACCATGCCCGGGAACTGGGAACGACCTTTCACGCCGCAAAACGCGGAACCTTTCCATACGCGACCAGTGACCAACTGGAAAGGACGGGTGGAGATTTCCTGACCGGc > 1:344030/1‑241 (MQ=255) cAGTAACGAATTACGGTTCGAATGTAGTGGTCTTCAGGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCACATGGTATGCGTGACAAACGGTTCCAGATCGATATc < 1:504430/110‑1 (MQ=255) aCGAATNACGGTTCGAATGGATTTGTCTTCATGCATCAGGTCGAAGGCGTCNNTNNNTNCATCCAgg < 2:362969/67‑1 (MQ=255) cGAATTACGGTTCGAATGGATTTGTCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATGGTATGCGTGACAAACGGTTCCAGATCGATATCACCTTTCATCGCATCTTCAACCATGCCCGGTAACTGGGAACGACCTTTCACGCCGCCaaccg > 1:40310/1‑163 (MQ=255) | GTGGGAGATAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATGGTATGCGTGACAAACGGTTCCAGATCGATATCACCTTTCATCGCATCTTCAACCATGCCCGGTAACTGGGAACGACCTTTCACGCCGCCAAACGCGGAACCTTTCCATACGCGACCAGTGACCAACTGGAATGGACGGGTGGAGATTTCCTGACCGGC > NC_000913/378263‑378698 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |