| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,362,490 | G→A | intergenic (+22/‑253) | puuR → / → puuC | DNA‑binding transcriptional repressor PuuR/gamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,362,490 | 0 | G | A | 90.9% | 21.1 / NA | 11 | intergenic (+22/‑253) | puuR/puuC | DNA‑binding transcriptional repressor PuuR/gamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (4/6); minor base C (1/0); total (5/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
GAATTAAGCATCAGGGTGAGGAAATAGGCACTGTACTGGAAGGTGAAATTGTTCTGACGATTAATGGTCAGGATTACCACCTCGTCGCGGGGCAAAGTTATGCCATTAATACCGGCATCCCGCACAGTTTCAGTAATACGTCGGCAGGTATTTGCCGAATTATCAGCGCCCATACGCCCACCACGTTTTAATCTTTTTGTTCTGTAAGCCGGGTAAGCGCAATGCGCCCGGCAATCTATACACAAAATCATTCAAGTTGCATCAAGGCGGCAAGTGAGCGAATCCCGATGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATGAAGTGTATATAAAGAATGTCGCCAGAAATAAACGGGCATAC > NC_000913/1362278‑1362672 | gAATTAAGCATCAGGGTGAGGAAATAGGCACTGTACTGGAAGGTGAAATTGTTCTGACGATTAATGGTCAGGATTACCACCTCGTCGCGGGGCAAAGTTATGCCATTAATACCGGCATCCCGCACAGTTTCAGTAATACGTCGGCAGGTATTTGCCGAATTATCAGCGCCCATACGCCCACCACGTTTTAATCTTTTTGTTCTGTAAGCCGGATAAGCGCAAtn < 1:103541/224‑2 (MQ=255) ggTCAGGATTACCACCTCGTCGCGGGGCAAAGTTATGCCATTAATACCGGCATCCCGCACAGTTTCAGTAATACGTCGGCAGGTATTTGCCGAATTATCAGCGCCCATACGCCCACCACGTTTTAATCTTTTTGTTCTGTAAGCCGGATAAGCGCAATGCGCCCGGCAATCTATACACAAAATCATTCAAGTTGCATCAAGGCGGCAAGTGAGCGAATCCCGATGAGCTTACTCAGGTAAGTGATTCgggg < 1:41252/251‑1 (MQ=255) gtcgCGGGGCAAAGTTATGCCATTAATACCGGCATCCCGCACAGTTTCAGTAATACGTCGGCAGGTATTTGCCGAATTATCAGCGCCCATACGCCCACCACGTTTTAATCTTTTTGTTCTGTAAGCCGGATAAGCGCAATGCGCCCGGCAATCTATACACAAAATCa > 1:435648/1‑167 (MQ=255) gcgGGGCACAGTTATGCCATTAATACCGGCATACCGCACCGTTTCAGTAATACGTCGGCAGGTATTTGCCGAATTATCAGCGCCCATACGCCCACAACGTTTTAATCTTTTTGTTCTGTAAGCAGGATAAGCGCACTGCGCCCGGCACTCTATACACAAAATCATTCAAGTTGCATCAAGGCGGCAAGTGAGCGAATCCCGATGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGc > 1:421361/1‑249 (MQ=255) gTCGGCAGGTATTTGCCGAATTATCAGAGCCCATACGCCCCCCACGTTATAATCTTTTTGTTCTCTCCGCCGACTTAGCGCAATGCGACCGGGACTCTATACACAAAATCATTCAAGTTGAATCAAGGCGGAAAGTGAGCGAATCCCGATGAGCTTACTAAGGTAAGTGATTCGGGGGAGAGGACGCAGCCAAGGCAGAGGCGGATTGAAGGATGAAGTGTATATAAAGAAAGTCGCCCGAAATAAACggg > 1:350074/1‑251 (MQ=255) cGGCAGGTATTTGCCGAATTATCAGCGCCCATACGCCCACCACGTTTTAATCTTTTTGTTCTGTACGCAGGATAAGCGCAATGCGCCCGGCAATATATACACAAAATcct > 1:247099/1‑108 (MQ=255) gCAGGTATTTGCCGAATTATCAGCGCCCATACGCCCACCACGTTTTAATCTTTTTGTTCTGTAAGCCGGATAAGCGCAATGCGCCCGGCAATCTATACACAAAATCAGTCAAGTTGCAGCAAGGCGGCAAGTGAGCGAATCCCGATGAGTTTACTCAGGGAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATGAAgtg < 1:235268/216‑1 (MQ=255) ggTATTTGCCGAATTATCAGCGCCCATACGCCCACCACGTTTTAATCTTTTTGTTCTGTAAGCCGGATAAGCGCAATGCGCCCGGCAATCTATACACAAAATCATTCAAGTTGCATCAAGGCGGCAAGTGAGCGAATCCCGATGAGCTTACTCAgg < 1:274216/156‑1 (MQ=255) gcgcCCNNACGCCCACCNCGTTTTAATCTTTTTGTTCTGTAAGCCGGATAAGCGCAATGCGCCCGGCAATCNATACACAAANNCANTCAATNTNNNTNNNGGNGGCAAGTGAGCGAATCCCGNTGNNCTTACTNAGGTAAGGGATTCGGGGGAGCGAACg < 2:107838/160‑1 (MQ=255) gcgcCCATACGCCCACCACGTTTTAATCTTTTTGTTCTGTAAGCCGGATAAGCGCAATGCGCCCGGCAATCTATACACAAAATCATTCAAGTTGCATCAAGGCTGCAAGTGAGCGAATCCCGATGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACg > 1:107838/1‑160 (MQ=255) cATACGCCCACCACGTTTTAATCTTTTTGTTCTGTAAGCCGGATAAGCGCAATGCGCCCGGCAATCTAGACACAAAATCATTCAAGTTGCATCAAGGCGGCAAGGGAGCGAATCCCGATAAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGGAGCCAAGGCAGAGGCGGCTAGAAGGATGAAGTGTATATAAAGAATGTCGCCAGAAATAAACGGGCATAc < 1:271063/225‑1 (MQ=255) | GAATTAAGCATCAGGGTGAGGAAATAGGCACTGTACTGGAAGGTGAAATTGTTCTGACGATTAATGGTCAGGATTACCACCTCGTCGCGGGGCAAAGTTATGCCATTAATACCGGCATCCCGCACAGTTTCAGTAATACGTCGGCAGGTATTTGCCGAATTATCAGCGCCCATACGCCCACCACGTTTTAATCTTTTTGTTCTGTAAGCCGGGTAAGCGCAATGCGCCCGGCAATCTATACACAAAATCATTCAAGTTGCATCAAGGCGGCAAGTGAGCGAATCCCGATGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATGAAGTGTATATAAAGAATGTCGCCAGAAATAAACGGGCATAC > NC_000913/1362278‑1362672 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |