| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,648,720 | C→T | A19V (GCC→GTC) | ydfB → | uncharacterized protein YdfB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,648,720 | 0 | C | T | 94.7% | 51.3 / NA | 19 | A19V (GCC→GTC) | ydfB | uncharacterized protein YdfB |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (11/7); minor base G (0/1); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTCGACACAATCATGGAAAAGGCTTACGAAGAATACTTCGAAGGCCTTGCCGAAGGCGAAGAAGCTCTCAGCTTCAGTGAGTTTAAACAGGCGCTTTCCAGTTCGGCAAAATCTAACGGCTGATAAGCGAAACAGCACCGCGAGGAATCAGTATGCAGAAACGAGAACCCGTCATCATCGCGCCAGACTATACCGATGATGAACTTTATGAGTGGATGCGCCAGAAAATTAATGCAGCGCAGGATCTGAAATG > NC_000913/1648525‑1648923 | tGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTTAGTGATATGTATTTCGACACAATCATGGAAAAGGCTTACGAAGAATACTGCGAAGGCCTGGTCGAAGGCGAAGAAGCGCTCAGCTTCAGTGAGTTTAAACATGCGCTTTCCAGtcc < 1:476672/250‑3 (MQ=255) caacaaCGAATCTCTGGTGTACGGCGTGTTTCCAAACCAAGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAACCCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTCGACACAATCATGGAAAAGGCTTACGAAGAATACTTCGAAGGCCTTGTCGAAGGCga > 1:425994/1‑202 (MQ=255) gACGGCACGTTCACCGCAATGACGTATACCAAAAGCGAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTCGACACAATCATGGAAAAGGCTTACGAAGAATACTTCGAAGGCCTTGTCGAAGGCGAAGAAGCTCTCAGCTTCAGTGAGTTTAAACAGGCGCTTGCCAGTTCGGCAAAATCTAACGGCTGATAAGc < 1:401797/231‑1 (MQ=255) cGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTCGACACAATCATGGAAAAGGCTTACGAAGAATACTTCGAAGGCCTTGTCGAAGGCGAAGAAGCTCTCAGCTTCAGTGAGTTTAAACAGGCGCTTTCCAGTTCGGCAAAATCTAACGGCTGATAAGCGAAACANc < 1:396162/225‑1 (MQ=255) ataCCAACAGCAAACCGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGAGATATGGATTTCGACACAATCATGGAAAAGGCTTACGAAGAATACTTCGAAGGCCTTGTCGACGGCGACGAAGCACTCAGCTTCAGTGAGTTTAAACAGGCGCTTTCCAGTTCGGCAAAATCTCACGGCTGCTAAg > 1:296194/1‑205 (MQ=255) gCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTCGACACAATCATGGAAAAGGCTTACGAAGAATACTTCGAAGGCCTTGTCGAAGGCGAAGAAGCTCTCAGCTTCAGTGAGTTTAAACAGGCGCTTTCCAGTTCGGCAAAATCTAACGGCTGATAAGCGAAACAGCACCGCGAGGAATCAGTATGCAGAAACGAGAAccc < 1:166157/239‑1 (MQ=255) ggctggAAAGAAACTCAGGTGAGTGANATGGATNNCGNCACAATCATGGAAAAGGCTNACNNNGNNNANTTCGANGGNNTTGTCGAAGNCGAAGAAGCTCTCAGCTTCAGTGAGTTTAAACAGGCGCTTTCCTGTTc > 2:118981/1‑137 (MQ=255) ggctggAAAGAAACTCAGGTGAGTGAGATGGATTTCGACACAATCATGGAAAAGGCTTACGAAGAATACTTCGAAGGCCTTGGCGAAGGCGAAGAAGCTCTCAGCTTCAGTGAGTTTAAACAGGCGCTTTCCAGTTc < 1:118981/137‑1 (MQ=255) gaaagaaaCTCAGGTGAGTGATATGGATTTCGACACAATCATGGAAAAGGCTTACGAAGAATACTTCGAAGGCCTTGTCGAAGGCGAAGAAGCTCTCAGCTTCAGTGAGTTTAAACAGGCGCTTTCCAGTTCGGCAAAATCTAACGGCTGATAAGCGAAACAGCACCGCGAGGAATCAGTATGCAGAAACga > 1:248472/1‑192 (MQ=255) aCTCAGGTGAGTGATATGGATTTCGACACAATCATGGAAAAGGCTTACGAAGAATACTTCGAAGGCCTTGTCGAAGGCGAAGAAGCTCTCAGCTTCAGTGAGTTTAAACAGGCGCTTTCCAGTTCGGCAAAATCTAACGGCTGATAAGCGAAACAGCAcc > 1:36762/1‑160 (MQ=255) tttCGACACAATCATGGAAAACGCTTACGAAGAATACTTCGAAGGCCTTGTCGAAGGCGAAGAAGCTCTCAGCt < 1:296257/74‑1 (MQ=255) ngACACAATCATGGAAAAGGCTTACGAAGAATACTTCGAAGGCCTTGTCGAAGGCGACGAAGCTCTCAGCTTCAGTGAGTTTAAACAGGCGCTTTCCAGTTCGGCAAAATCTAACGGCTGATAAGCGAAACAGCACCGCGAGGAATCAGTATGCAGAAACGAGAACCCGTCatcatc > 1:57097/2‑177 (MQ=255) cGACACAATCATGGAAAAGGCTTACGAAGAATACTTCGAAGGCCTTGTCGAAGGCGAAGAAGCTCTCAGCTTCAGTGAGTTTAAACAGGCGCTTTCCAGTTCGGCAAAATCTAACGGCTGATAAGCGAAACAGCACCGCGAGGAATCAGTATGCAGAAACGAGAACCCGTCATCATCGCGCCAGACTATACCGATGATGAACTTTATGAGTGGATGCGCCAGAAAAt > 1:350371/1‑227 (MQ=255) cGACACAATAATGGAAAAGGCTTACGAAGAATACTTCGAAGGCCTTGTCGAAGGCGAAGAAGCTCTCAGCTTCAGTGAGTTTAAACAGGCGCTTTCCAGTTCGGCAAAATCTAACGGCTGATAAGCGAAACAGCACCGCGAGGAATCAGTATGCAGAAACGAGAACCCGTCATCATCGCGCCAGACTATACCGATGATGAACTTTATGAGTGGATGCGCCAGAAAATTAATGCAGCGCAGGATCTGAAATg > 1:109324/1‑251 (MQ=255) acacAATCATGGAAAAGGCTTACGAAGAATACTTCGAAGGCATTGTCGAAGGCGAAGAAGCTCTCAGCTTCAGAGAGTTTaaa > 1:222706/1‑83 (MQ=255) cATGGAAAAGGNTTACGAAGAANACTNNGAAGNCNTNGTCAAAGGCGAAGAAGCTCGCAGCTTCAGNGAGTTTAAACAGGCGCTTTCCAGNNCGNNNATNNCTAAc < 2:374055/106‑1 (MQ=255) cATGGAAAAGGCTTACGAAGAATACTTCGAAGGCCTTGTCGAAGGCGAAGAAGCTCTCAGCTTAAGTGAGTTTAAACAGGCGCTTTCCAGTTCGGCAAAATCTAAc > 1:374055/1‑106 (MQ=255) tGGAANAGGCTNANNAAGAATACTTCGAAGGCCTNGTCGAAGGCGAAGAANCTCTNAGCTTNAGNg > 2:216660/1‑66 (MQ=255) tnANGAAGAATACTTCGAAGGCCTTGTCGAAGGCGAAGAAGCNCTCANCNNCANTNATNTTaaa < 2:222706/64‑1 (MQ=255) | TGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTCGACACAATCATGGAAAAGGCTTACGAAGAATACTTCGAAGGCCTTGCCGAAGGCGAAGAAGCTCTCAGCTTCAGTGAGTTTAAACAGGCGCTTTCCAGTTCGGCAAAATCTAACGGCTGATAAGCGAAACAGCACCGCGAGGAATCAGTATGCAGAAACGAGAACCCGTCATCATCGCGCCAGACTATACCGATGATGAACTTTATGAGTGGATGCGCCAGAAAATTAATGCAGCGCAGGATCTGAAATG > NC_000913/1648525‑1648923 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |