Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,132,865	G→A	G98R (GGA→AGA)	flgE →	flagellar hook protein FlgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,132,865	0	G	A	100.0%	43.5 / NA	16	G98R (GGA→AGA)	flgE	flagellar hook protein FlgE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/10);  total (6/10)

GCCAACTCCGCCACCTACGGCTTTAAATCAGGCACGGCCTCTTTTGCCGATATGTTTGCCGGTTCGAAAGTGGGACTGGGGGTAAAAGTTGCCGGTATCACTCAGGACTTTACCGATGGCACGACCACCAACACCGGGCGAGGTCTGGACGTTGCTATCAGCCAGAACGGTTTTTTCCGTCTGGTAGACAGCAACGGTTCGGTGTTCTACAGCCGTAACGGACAATTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGTTACCCGGCAACCGGTACGCCGCCGACTATTCAGCAAGGGGCGAATCCGACCAATATTTCGATCCCGAATACCCTGATGGCAGCGAAAACTACCACCACGGCATCGATGCAGATCAACCTGAATTCCAGTGATC  >  NC_000913/1132646‑1133060                                                                                                                                                                                                                            |                                                                                                                                                                                                    gCCAACTACACCACCTACGGCTTTAAATCAGGCACGGCCTCTTTTGCCGATATGTTTGCCGGTTCGAAAGTGGGACTGGGGGTAAAAGTTGCCGGTATCACTCAGGACTTTACCGATGGCACGACCACCAACACCGGGCGAGGTCTGGACGTTGCTATCAGCCAGAACGGTTTTTTCCGTCTGGTAGACAGCAACGGTTCGGTGTTCTACAGCCGTAACAGACAATTTAAGCTGGATGAAAACCGTAACCt                                                                                                                                                                      >  1:398657/1‑251 (MQ=255)                                                                  gAAAGTGGGACTGGGGGTAAAAGTTGCCGGTATCACTCAGGACTTTACAGAGGGCACGACCACCAACACCGGGCGAGGTCTGGACGTTGCTATCAGCCAGAACGGTTTTTTCCGTCTGGTAGACAGCAACGGTTCGGTGTTCTACAGCCGTAACAGACAATTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTAc                                                                                                                                                  <  1:182843/206‑1 (MQ=255)                                                                                           gCCGGTATCACGCAGGACTTTACCGATGGCACGACCACCAACACCGGGCGAGGTCTGGACGTTGCTATCAGCCAGAACGGTTTTTTCCGTCTGGTAGACAGCAACGGTTCGGTGTTCTACAGCCGTAACAGACAATTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGc                                                                                                                                               <  1:85393/184‑1 (MQ=255)                                                                                                                aCCGATGGCACGACCACCAACACCGGGCGAGGTCTGGACTTTGCTAGCAGCCAGAACGGTTTTTTCCGTCTGGTAGACAGCAACGGTTCGGTGTTCTACAGCCGTAACAGACAATTTAAGCTGGATGAAAACCGTAACCTG‑TGAATATGCAAGGTTTACAGCTGACCGGTTACCCGGCAACCGGGACGCGGCCGACTATTCTGCAAGGGGCGAATCCGTCCAATATTTCGATCCCGAATACCCTGATggn                                                       <  1:58501/250‑2 (MQ=255)                                                                                                                         aCGACCACCAACCCCGGGCGAGGTCTGGACGTTGCGATCAGCCAGAACGGTTTTTTCCGTCTGGTAGACAGCAACGGTTCGGTGTTCTACAGCCGTAACAGACAATTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGTTACCCGGCAACCGGTACGCCGCCGACTATTCAGCAAGGGGCGAATCCGACCAATATTTCGATCCCGAATACCCTGATGGCAGCGAAAAc                                              <  1:38021/251‑1 (MQ=255)                                                                                                                                ccaACACCGGGCGAGGTCTGGCCGTTGCTATCAGCCAGAACGGTTTTTTCCGTCTGGTAGACAGCAACTGTTCGGTGTTCTAGAGCCGTAACATACAATTTAAGCTGGATGAAAACCGTACCCTGGTTAATATGCATGGTTTACAGCTGACGGGTTACCCGGCAACCGGTACGCCGCCGACTATTCAGCAAGGGGCGAATc                                                                                         >  1:106741/1‑201 (MQ=255)                                                                                                                                ccaACACCGGGCGAGGTCTGGACGTTGCTATCAGCCAGAACGGTTTTTTCCGTCTGGTAGACAGCAACGGTTCGGTGTTCTACCGCCGTAACAGACAATTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCCAGGTTTACAGCTGACGGGTTACCCGGCAACCGGTACGCCGCCGACTATTCAGCAAGGGGCGAATCCGACCAATATTTCGCTCCCGAATACCCTGATGGCAGCGAAAACTaccacc                                       >  1:75469/1‑251 (MQ=255)                                                                                                                                      ccGGGCGAGGTCTGGACGTTGCTATCAGCCAGAACGGTTTTTTCCGTCTGGGAGACAGCAACGGTTCGGTGTTCTACAGCCGTAACAGACAATTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGTTACCCGGCAACCGGTAc                                                                                                                      <  1:462418/166‑1 (MQ=255)                                                                                                                                        nggNGAGNTNNGNNCGTNGCTGTCAGCCAGAACGGTTTTNNCCGTCTGGTNGACAGCAACGGTTCGGTGTTCTACAGCCGTAACAGACAATTTAAGCTGGATGANAACCGTAACNNGGNGAATANGNNNGNNNTANAGCTGACGGGTTACCCGGCNACNNGTACGCNGCCGACTATTCAGCAAGGGGCGAATc                                                                                         <  2:106741/192‑1 (MQ=255)                                                                                                                                               gTCTGGACGTTGCTATCAGCCAGAACGGTTTTTTCCGTCTGGTAGACAGCAACGGTTCGGTGTTCTACAGCCGTAACAGACAATTTAANNTGGATGnna                                                                                                                                                                                >  2:24406/1‑99 (MQ=255)                                                                                                                                                          gCTACCAGCAAGAACTGTTTTTTCCGTCTGGTAGACAGCAACGGTTCGGTTTTCTGCAGCCGTAACAGACAATTTAAGCTGGATGAAAGCCGTAGCCGGGTGACTATGCAAGGTTTACAGCGGGCGGGTTACCCGGCATCCGTTACGCCGCCGACTTTTCAGCAAGGGGCGAATCCGACCAAGATTTCGATCCCGAATGCCTTGATGGCAGAGAAAACTTCCCCCTCGGCATCGATGCAGATCATCCTGaa             <  1:24406/251‑1 (MQ=255)                                                                                                                                                             aTCAGCCAGAACGGTTTTTTCCGTCTGGTAGACAGCAACGGTTCGGTGTTCTTCAGACGTAACAGACAATTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGCAGGGTTAACCGGCAACCGGTACGCCGCCGACTATTCAGCAAGGGGCg                                                                                             >  1:241933/1‑168 (MQ=255)                                                                                                                                                               cAGCCAGAACGGTTTTTTCCGTCGGGTAGACAGCAACGGTTCGGTGTTCTACAGCCGTAACAGACAATTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGTTACCCGGCAACCGGTACgccgcc                                                                                                                <  1:385057/147‑1 (MQ=255)                                                                                                                                                                           ttttttCCGTCGGGTAGACAGCAACGGTGCGGTGTTCGACAGCCGGAACAGACAATTTAAGCTGGATTAAAACCGTAACCTGGTGAATATGCAAGTTATACAGCTGACGGGTTACCCGGCAACCGGTACGCCTCCGACTATTCAGCAAGGGGTTAAGCCGACCAAGATGTCGATCCCGAATACCCTGATGGCATCTAAAACTACCACCACGGCATCGATGCAGATCAACCTGAATTCCAGTGATc  <  1:281007/245‑1 (MQ=255)                                                                                                                                                                                               gCAACGGTTCGGTGTTCTACAGCCGTAACAGACAATTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGttt                                                                                                                                                    >  2:16434/1‑79 (MQ=255)                                                                                                                                                                                               gCAACGGTTCGGGGTTCTACAGCCGTAACAGACAAGTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGttt                                                                                                                                                    <  1:16434/79‑1 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                    GCCAACTCCGCCACCTACGGCTTTAAATCAGGCACGGCCTCTTTTGCCGATATGTTTGCCGGTTCGAAAGTGGGACTGGGGGTAAAAGTTGCCGGTATCACTCAGGACTTTACCGATGGCACGACCACCAACACCGGGCGAGGTCTGGACGTTGCTATCAGCCAGAACGGTTTTTTCCGTCTGGTAGACAGCAACGGTTCGGTGTTCTACAGCCGTAACGGACAATTTAAGCTGGATGAAAACCGTAACCTGGTGAATATGCAAGGTTTACAGCTGACGGGTTACCCGGCAACCGGTACGCCGCCGACTATTCAGCAAGGGGCGAATCCGACCAATATTTCGATCCCGAATACCCTGATGGCAGCGAAAACTACCACCACGGCATCGATGCAGATCAACCTGAATTCCAGTGATC  >  NC_000913/1132646‑1133060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑