Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,286,554	C→T	pseudogene (2361/2525 nt)	yejO ←	adhesin‑like autotransporter YejO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,286,554	0	C	T	100.0%	46.9 / NA	19	pseudogene (2361/2525 nt)	yejO	adhesin‑like autotransporter YejO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/11);  total (8/11)

TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGACAAATGACCGCTTAACGTCGGGGTAAACGATGACCTTATACCAGCCTGATAAACGCCACTGGTTCCAGCCACATCATTATTAAATTTGCCATCGTCATTAACTTTCACCTGGTTAGAATCGGCGTATTCCTGACGCACGGCCGCTTTCAGCCAGGGTTCCAGCGTCGTACCGTTTTGCAGGTCCATGTGATAGCTTACCGCCGTTCCCGCTTCAGCGCGTAATATCCGGGTATTTCCCACATCCGCGCGCATGCCGTTTGATAACGTGTAGTCCTGACCATCTGTGGTAAAGCCGGTAAAGGCCAGATAG  >  NC_000913/2286323‑2286774                                                                                                                                                                                                                                        |                                                                                                                                                                                                                             ttttttATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGACAAATGACCGCTTAACGTCGGGGTAAACGATGACCTTATACCAGCCTGATAAACGCCACTGGTTCCAGCCACATCATTATTAAATTTGCTATCGTCATTAACTTTCAc                                                                                                                                                                                                            <  1:223568/250‑1 (MQ=255)           cTGCAATTAATTCGATAAACAGAACGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGACAAATGACCGATTAACGTCGGGGTAAACGATGACCTTATACCAGCCTGATAAACGCCACTGGTTCCAGCAACATCATTATTAAATTTGCTATCGTCATTAACTTTCAcc                                                                                                                                                                                                           >  1:448484/1‑241 (MQ=255)                                        cacaTCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGACAAATGACCGCTTAACGTCGGGGTAAACGATGACCTTATACCAGCCTGATAAACGCCACTGGTTCCAGCCACATCATTAGTAAATTTGCTATCGTCATTAACTTTCGCCTGGTTAGAATc                                                                                                                                                                                                <  1:260906/223‑1 (MQ=255)                                                             tCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGACAAATGACCGCTTAACGTCGGGGTAAACGATGACCTTATACCAGCCTGATAAACGCCACTGGTTCCAGCCACATCATTATTAAATTTGCTATCGTCATTAACTTTCACCTGGTTAGAATCGGCGTATTCCTGACGCACGGCCGCTTTCAGCCAGGGTTCCAGcgtcgt                                                                                                                                                <  1:230502/250‑1 (MQ=255)                                                                gTTATCAGAACGTCCAGACCACTCCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGACAAATGACCGCTTAACGTCGGGGTAAACGATGACCTTATACCAGCCTGATAAACGCCACTGGTTCCAGCCACATCATTATTAAATTTGCTATCGTCATTAACATTCACCTGGTTAGAATCGGCGTATTCCTGACGCACGGCCGCTTTCAGCCAGGGTTCCAGCGTCGTAcc                                                                                                                                             <  1:7045/250‑1 (MQ=255)                                                                         aCGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGATGACTGACAAATGACTGCTTAACGTCGGGGTAAAAGATGACCTTATACCAGCCTGATAAACGCCACTGGTTCCAGCCACATCATTATTAAATTTGCTATCGTCATTAACTTTCACCTGGTTAGAATCGGCGTATTCCTGACGCACGGCCGCTTTCAGCCAGGGTTCCAGCGTCGTACCGTTTTGCAgg                                                                                                                                   >  1:372182/1‑251 (MQ=255)                                                                                      ccncGCNTNNGNNTTCNACGGCGATTCTACCCCTGCGCNNTTGCCATAGNTGACTGACAAATGACCGCTTAACGTCGGGGTAAACGATGACCTTATACCAGCCNGATAAACGCNNCTNGTTCCNGNNNCNNNATNATTAAATTTGCTATCGTCANTANNTTTCACNTGGTTAGAATCGGCGTATTCCTGACg                                                                                                                                                                                 <  2:109425/191‑1 (MQ=255)                                                                                          cccTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGTCATAGCTGACTTACAAATGACCGCTTAACGGCGGGGTAAACGATGACCTTATACCAGCCTTATAAACGCCACTGGGTCCAGCCACATCATTATTAAATTTGCTATCGTCATTAACTTTCTCCGTGTAAGAATCGGCGTATTCCTGACGCACGGCCGCTTTCAGCCAGGGTTCCAGCGTCGTACCGTTTTGCAGGTCCATGTGATAGCTTAc                                                                                                                  <  1:347041/250‑1 (MQ=255)                                                                                           ccTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGACAAATGACCGCTTAACGTCGGGGTAAACGCTGACCTTATACCAGCCTGATAAACGCCACTGGTTCCAGCCACATCATTATTAAATTTGCTATCGTCATTAACTTTCACCTGGTTAGAATCGGCGTATTCATGACGCACGGCCGCTTTCAg                                                                                                                                                                  >  1:382640/1‑202 (MQ=255)                                                                                                     ncACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGACAAATGAACGCTTAACGTCGGGGTAAACGATGACCTTATACCAGCCTGATAAACGCCACTGGTTCCAGCCACATCATTATTAAATTTGCTATCGTCATTAACTTTCACCTGGTTAGAATCGGCGTATTCCTGACGCACGGCCGCTTTCAGCCAGGGTTCCCGCGTCGTACCGTTTTGCAGGTCCATGTGATAGCTTACCGCCGTTCCCg                                                                                                       >  1:105138/2‑251 (MQ=255)                                                                                                                                 ccATAGCTGACTGACAAATGACCGCTTAACGTCGGGGTAAACGATGACCTTATACCAGCCTGATAAACGCCACTGGTTCCAGCCACATCATTATTAAATTTGCTATCGTCATTAACTTTCACCTGGTTAGAATCGGCGTAt                                                                                                                                                                                         <  1:251650/141‑1 (MQ=255)                                                                                                                                       ctgactgaCAAATGACCGCTTAACGTCGGGGTAAACGATGACCTTATACCAGCCTGATAAACGCCACTGGTTCCAGCCACATCATTATTAAATTTGCTATCGTCATTAACTTTCACCTGGTTAGAATCGGCGTATTCCTGACGCACGGc                                                                                                                                                                           <  1:465069/149‑1 (MQ=255)                                                                                                                                                                     gTAAACGATGACCTTATACCAGCCTGATAAACGCCACTGGTTCCAGCCACATCATTATTAAATTTGCTATCGTCATTAACTTTCACCTGGTTAGAATCGGCGTATTCCTGACGCACGGcc                                                                                                                                                                          <  1:280166/120‑1 (MQ=255)                                                                                                                                                                        naCGATGACCTTATACCAGCCTGATAAACGCAACTGGTTACAGCCACATCATTATTAAATTTGCTATCGTCATTAACTTTAACATGGTTAGAATCGGc                                                                                                                                                                                             >  1:51887/2‑98 (MQ=255)                                                                                                                                                                           gATGACCTTATACCAGCCTGATAAACGCCACTGGTTCCAGCCACATCATTATTAAATTTGCTATCGTCATTAACTTTCACCTGGTTAGAATCGGCGTATTCCTGACGCACGGCCGCTTTCAGCCAGGGTTCCAGCGTCGTACCGTTTTGCAGGTCCAGGGGATAGCTTACCGCCGTTCCCGCTTCAGCGCGTAATATCCGGGTATTTCCCACATCCGCGCGCATGc                                                          <  1:36565/226‑1 (MQ=255)                                                                                                                                                                                ccTTATACCAGCCTGATAAACGCCACTGGTTCCAGCCACATCATTATTAAATTTGCTATCGTCATTAACTTTCACCTGGTTAGAATCGGCGTATTCCTGACGCACGGCCGCTTTCAGCCAGGGTTCCAGCGTCGTACCGTTTTGCAGGTCCATGTGATAGCTTACCGCCGTTCCCGCTTCAGCGCGTAATATCAGGGTATTTCCCACATCCGCGCGCATGCCGTTTGATAACGTGTAGTCCTGACCATCtg                            >  1:211002/1‑251 (MQ=255)                                                                                                                                                                                                  aaCGCCCCTGGTTCCAGCCACATCATTATTAAATTTGCTATCGTCATTAACTTTCTCCTGGTTAGAGTCGGCGTCTTCCTGACGCACGGCGGCTTTCAGACAGGGTTCCAGCGGCGTCCCGTTTTGc                                                                                                                                      >  1:430202/1‑127 (MQ=255)                                                                                                                                                                                                    cGCCACTGGTTCCAGCCACATCATTATTAAATTTGCTATCGTCATTAACTTTCACCTGGTTAGAATCGGCGTATTCCTGACGCACGGCCGCTTTCAGCCAGGGTTCCAGCGTCGTACCGTTTTGCAGGTCCATGTGATAGCTTACCGCCGTTCCCGCTTCAGCGCGTAATATCCGGGTATTTCCCACATCCGCGCGCATGCCGTTTGATAACGTGTAGTCCTGACCATCTGTGGTAAAGCCGGTAAAGGcc        >  1:42834/1‑251 (MQ=255)                                                                                                                                                                                                           ggTTCCAGCCAAATCATTATTAAATTTTCTATCGTCATTAACTTTCACCGGGTGAGAATCGGCGTATTCCTGACGCCCGGCCGCTTTCAGCCAGGGTTCCAGCGTCGTACCTTTTTGCAGGTCCATGTGATAGCTTACCGCCGTTCCCGCTTCAGCGCGTAATATCCGGGTATTTCCCACATCCGCGCGCATGCCGTTTGATAACGTGTAGTCCTGACCATCTGTGGTAAAGCCGGTAAAGGCCAGATan  <  1:150169/250‑2 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                             TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGACAAATGACCGCTTAACGTCGGGGTAAACGATGACCTTATACCAGCCTGATAAACGCCACTGGTTCCAGCCACATCATTATTAAATTTGCCATCGTCATTAACTTTCACCTGGTTAGAATCGGCGTATTCCTGACGCACGGCCGCTTTCAGCCAGGGTTCCAGCGTCGTACCGTTTTGCAGGTCCATGTGATAGCTTACCGCCGTTCCCGCTTCAGCGCGTAATATCCGGGTATTTCCCACATCCGCGCGCATGCCGTTTGATAACGTGTAGTCCTGACCATCTGTGGTAAAGCCGGTAAAGGCCAGATAG  >  NC_000913/2286323‑2286774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑