Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,015,879	A→G	D97D (GAT→GAC)	ysgA ←	putative dienelactone hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,015,879	0	A	G	100.0%	65.9 / NA	22	D97D (GAT→GAC)	ysgA	putative dienelactone hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/7);  total (15/7)

CAGTTTGCCGTACCACGCCACTGCGGCTTTTAGCTGTGGATTATGCGCGGCATACAGCCAGGTGATACGTCCACCCCAGCAGAATCCGGTGATCATTAAACGATGAACATCGCCGCCGTTGCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGC  >  NC_000913/4015651‑4016091                                                                                                                                                                                                                                     |                                                                                                                                                                                                                     cAGTTGGCCGTACCACGCCCCTGCGGCTTTTAGCTGTGGATTATGCGCGGCATACAGCCAGGTGATACGTCCACCCCAGCAGAATCCGGTGATCATTAAACGATGAACATCGCCGCCGTTGCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGGACCTGCTAGTCAGGCACTTTTGCGACCAGACCGCCAATCAACGTGGGGATATCGGCAAAATCATTCGGGTCGCCTTCGCGGAAGTAAAGtt                                                                                                                                                                                                <  1:263874/251‑1 (MQ=255)         cGTACCACGCCACTTCGGCTTTTAGCTGTGGATTATGCGCGGCATACAGCCAGGTGATACGTCCACCCCAGCAGAATCCGGTGATCATTAAACGATGAACATCGCCGCCGTTGCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGGTCGCCTTcgc                                                                                                                                                                                                            <  1:259822/231‑1 (MQ=255)                                              cgcgGCATACAGCCAGGTGATACGTCCACCCCAGCAGAATCCGGTGATCATTAAACGATGAACATCGCCGCCGTTGCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGGTCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACgg                                                                                                                                                          >  1:397929/1‑244 (MQ=255)                                                   cATACAGCCAGGTGATACGTCCACCCCAGCAGAATCCGGTGATCATTAAACGATGAACATCGCCGCCGTTGCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGGTCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGCCGGCGAc                                                                                                                                                      >  1:377961/1‑243 (MQ=255)                                                       cAGCCAGGTGATACGTCCACCCCAGCAGCATCAGGTGATCATTAAACGATGAACATCGCCGCCGTTGCGGGCAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGGTCg                                                                                                                                                                                                                   >  1:413571/1‑178 (MQ=255)                                                                           cccAGCAGAATCCGGTGATCATTAAACGATGAACATCGCCGCCGTTGCGGGAAGCTCAACTGGCGACATGATCGCGATCGGCCAGCCCCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGCGGGGATATCGGCAAAATCATTCGGGTCGACTTCGCGGAAGTAAAGt                                                                                                                                                                                                 >  1:451170/1‑176 (MQ=255)                                                                                                            naTCGCCGCCGTTGCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCCAAATCATTCGGGTCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGata                                                                                                                                        >  1:90050/2‑200 (MQ=255)                                                                                                               cgccgcCGTTGCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGGTCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCTCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGt                                                                                        <  1:357787/245‑1 (MQ=255)                                                                                                                          cGGGAAGCCCAACTGGCGACATGATCGAGTTCGGCCAGCCCCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCGAAATCATTCGGGACGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCgctttgctt                                                                       >  1:164068/1‑251 (MQ=255)                                                                                                                           gggAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTCGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGGGCGCGTTCTCGGAAGTAAAGTTCAGGTGCGAAAGCCAGATACCCCGCCAGCGCCAGACGTGGACAAATGGCGCGGAAATGCGCATGCACTCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGc                                                                                    <  1:467465/237‑1 (MQ=255)                                                                                                                                                gATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCACAATCATTCGGGTCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGc                                                 >  1:240504/1‑251 (MQ=255)                                                                                                                                                                     gCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGGTCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAggg                            <  1:459603/251‑1 (MQ=255)                                                                                                                                                                                       cTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGGTCGCCTTCGCGGCAGTACAGTTCAGGTGCGATAGCCAGATTAAACTCCAGCGCAAGACGGc                                                                                                                                                         >  1:267742/1‑108 (MQ=255)                                                                                                                                                                                               ccGCTCAGAAACGTGGGGATTTCGGCAAAATAATTCGGGTCGCCTTCGCGGAAGTAAAGTTCAGGTg                                                                                                                                                                                          >  1:422808/1‑67 (MQ=255)                                                                                                                                                                                               ccGCTAAGNANNNTNNGGATATCGGCAAAATCATTCGGGTCGCCTTCGCGGAAGTAAAGTNCCGGTGCGATAGc                                                                                                                                                                                   >  2:358395/1‑74 (MQ=255)                                                                                                                                                                                               ccGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGGTCGCCTTCGCGGAAGTAAAGTTCGGGTGCGATCGACAGATACCCCTACAGCGCCAGACGCCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATcgc  >  1:374505/1‑251 (MQ=255)                                                                                                                                                                                               ccGCTAAGCAACGGGGGGATATCGGCAAAATCATTCGGGTCGCCTTCGCGGAAGTAAAGTTCATGTGCGATAGc                                                                                                                                                                                   <  1:358395/74‑1 (MQ=255)                                                                                                                                                                                                 gCTAANCAACGNGNNGATATCGGCAAAATCATTCNGGTCGCCTTCGCGGANGTAANGTTCANGTNc                                                                                                                                                                                         >  2:204851/1‑66 (MQ=255)                                                                                                                                                                                                 gCTAAGCAACGTGGGGATATCGGCAAAATCAGTCGGGTCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGc                                                                                    <  1:204851/167‑1 (MQ=255)                                                                                                                                                                                                  cTAAGCAACGTGGGGATATCGGCAAAATCATTCGGGTCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGat                                                                                                                                         >  1:689/1‑113 (MQ=255)                                                                                                                                                                                                               ggATATCGGCAAAATCATTCGGGTCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAgcgc                                                                                                                                                                 >  1:276440/1‑76 (MQ=255)                                                                                                                                                                                                               ggATATCGGCAAAATCATTCGGGTCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCa                                                                                                                                  >  1:134935/1‑107 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                     CAGTTTGCCGTACCACGCCACTGCGGCTTTTAGCTGTGGATTATGCGCGGCATACAGCCAGGTGATACGTCCACCCCAGCAGAATCCGGTGATCATTAAACGATGAACATCGCCGCCGTTGCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGC  >  NC_000913/4015651‑4016091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑