| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,692,949 | G→A | G865G (GGC→GGT) | purL ← | phosphoribosylformylglycinamide synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,692,949 | 0 | G | A | 94.4% | 40.6 / ‑4.2 | 18 | G865G (GGC→GGT) | purL | phosphoribosylformylglycinamide synthetase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (12/5); total (13/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
CGGACTCGACCGCTTCACGGTCAGCGGCACGAACCTGAATCACCGCACCCAGTTCTTCGTTAAACAACGCCGCCAGGCGATCGTCACCCAGAGTGGCGATATCCGCGTCAATGCCACAATGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCGGCGTGATGGTGTGACGTACATCTTCCACGCGGGCAAATGCAGAAATCACCAGCGACAGCGGCGACGTC > NC_000913/2692720‑2693162 | cGGACTCGACCGCTTCACGGTCAGCGGCACGAACCTGCATCACCGCACCCAGTTCTTAGTTAAACAACGCCGCCAGGCGATCGTCACACAGAGTGGCGATATCCGCGTCAATTCCACAATGACCAGCAGAGGCCATTTCCCCCCGCGTTCCCAGCAGGCCCCCATCAGAACGGTCGTGATAAGCCAGCAGCGTACGCTGGGCCACACGCGCCTGAATCGCGTCCTAGAAGCCTTTCAGTTGCGCGACCTcg > 1:262766/1‑251 (MQ=255) aCGAACCTGAATCACCGCACCCAGTTCTTCGTTAAACAACGCCGCCAGGCGATCGTCACCCAGAGTGGCGATATCCGCGTCAATGCCACAATGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAACCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCt > 1:437652/1‑251 (MQ=255) gccgccAGGCGATCGTCACCCAGAGTGGCGATATCCGCGTCAATGCCACAATGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCCGGCCGCCATCAGCGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAACCTTTCAGTTGCGCGACATCGCGTACATCTGcc > 1:263645/1‑195 (MQ=255) aTCGTCACCCAGAGTGGCGATATCCGCGTCAATGCCACAATGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCAGCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAACCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGc < 1:337463/251‑1 (MQ=255) cGTCACCCAGAGTGGCGATATCCGCTTCAATGCGACAATGACCAGCAACGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGACACCTTTCAGTTGCGCGACATCGCGTa > 1:407833/1‑174 (MQ=255) cccAGAGTGGCGATATCCGCGTCAATGCCACAATGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAACCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCa > 1:139981/1‑226 (MQ=255) tGGCGATATCCGCGTCAATGCCACAATGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAACCtt < 1:72159/141‑1 (MQ=255) tCCGCGTCAATGCCACAATGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAACCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTgcgcn < 1:305037/195‑2 (MQ=255) tCCGCGTCAATGCCACAATGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAACCTTNCAGNNNnncgnnnnnnnnnn > 2:305037/1‑144 (MQ=255) gCCACAATGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGACGTGATACGCCAGCAGCTTCCGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAACCTTTCAGTTGCGCGCCATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCCGCGCCGTTGCGCCCAGCGAGTTATTGCCTTTGCCCACATCACTCAGCACCCGTGCGTTATCTTCgg > 1:201240/1‑251 (MQ=255) ncAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAACCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATaaa > 1:308979/2‑147 (MQ=255) nngCNNNNNCAGNAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAACCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATaaa < 2:308979/144‑1 (MQ=255) gTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAACCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTa > 1:21298/1‑210 (MQ=255) ccAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGACACCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGcgcg > 1:80782/1‑167 (MQ=255) cgtatcacgaccgctctGATACGCCANCAGCTTNNGCNGTGCAACCAGCGCCTGAATNGCNNNANNNANACCTTNCANNTGCGCGACANCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCNTTGCGCCCANNGCGTTATTg > 2:118016/17‑163 (MQ=255) cgtatcacgaccgctctGATACGCCAGCAGCTTACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAACCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTg < 1:118016/147‑1 (MQ=255) aCGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAACCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCGGCGTGATGGTGTGACGTACATCTTCCACGCGGGCAAATGCAGAAATCACCAGCGACAGCGGCGACGTc > 1:24698/1‑250 (MQ=255) cgcCTGAATAGCGTCATAGAAACCTTTCAGTTGCGCGACATCGCGTACAACCGCCGGCTTGTCGACAAGCTGACGATCAACCTCCGCCAGCGCCGTTGCGCCCCGCGCGTTGTTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTCTCTTCGGTAGAAAGCTGCGGCGTGATGGCGTGACGTACAGCTTCCACGCGGGCAACTCCAGCAATCACCAGCAACAGAGGCAACg > 1:503291/1‑233 (MQ=255) | CGGACTCGACCGCTTCACGGTCAGCGGCACGAACCTGAATCACCGCACCCAGTTCTTCGTTAAACAACGCCGCCAGGCGATCGTCACCCAGAGTGGCGATATCCGCGTCAATGCCACAATGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCGGCGTGATGGTGTGACGTACATCTTCCACGCGGGCAAATGCAGAAATCACCAGCGACAGCGGCGACGTC > NC_000913/2692720‑2693162 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |