Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,541,225	G→A	A273A (GCG→GCA)	feoB →	Fe(2(+)) transporter FeoB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,541,225	0	G	A	100.0%	40.2 / NA	14	A273A (GCG→GCA)	feoB	Fe(2(+)) transporter FeoB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/8);  total (6/8)

CATCCCGCTGAAACAACGTCGCTGGCTGGGCCTGCAAATGCTGGAAGGCGATATCTACAGCCGCGCCTACGCCGGTGAAGCGTCGCAGCATCTGGATGCCGCCCTCGCCCGTCTGCGTAATGAGATGGACGATCCGGCGCTGCACATTGCCGATGCGCGTTACCAGTGCATTGCTGCCATCTGTGATGTGGTAAGCAACACCCTGACGGCAGAACCCAGCCGTTTCACCACTGCGGTAGATAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGTGCATGGTATTCAATGGATTGGCTACACGCTCC‑ACTTCCCGGACTGGCTGACTATCTT‑CCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGT  >  NC_000913/3540991‑3541455                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                         cATCCCGCTGAAACAACGTCGCTGGCTGGGCCTGCAAATGCTGGAAGGCGATATCTACAGCCGCGCCTACGCCGGTGAAGCGTCGCAGCATCTGGATGCCGCCCTCGCCCGTCGGCGTAATGAGATGGACGATCCGGCGCTGCACATTGCCGATGCGCGTTACCAGTGCATTGCTGCCATCTGGGATGTGGTAAGCAACACCCTGACGGCAGAACCCAGCCGTTTCACCACTGCAGTAGATAAAATCGTGc                                                                                                                                                                                                                          <  1:485339/251‑1 (MQ=255)                              gCCTGCAAATGCTGGAAGGCGATATCTACAGCCGCGCCTACGCCGGTGAAGCGTCGCAGCATCTGGATGCCGCCCTCGCCCGTCTGCGTAATGAGATGGACGATCCGGCGCTGCACATTGCCGATGCGCGTTACCAGTGCATTGCTGCCATCTGTGATGTGGTAAGCAACACCCTGACGGCAGAACCCAGCCGTTTCACCACTGCAGTAGATAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTTc                                                                                                                                                                                             >  1:121053/1‑251 (MQ=255)                              gCCTGCAAATGCTGGAAGGCGATATCTACAGCCGCGCCTACGCCGGTGAAGCGTCGCAGCATCTGGATGCCGCCCTCGCCCGTCTGCGTAATGAGATGGACGATCCGGCGCTGCACATTGCCGATGCGCGTTACCAGTGCATTGCGGCCATCTGTGATGTGGTAAGCAACACCCTTACGGCAGAACCCATCCGTTTCACCACTGCAGTAGATAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCg                                                                                                                                                                                                   <  1:342036/245‑1 (MQ=255)                                                                     aCGCCGGTGAAGCGTCGCAGCATCTGGATGCCGCCCTCGCCCGTCTGCGTAATGAGATGGACGATCCGGCGCTGCACATTGCCGATGCGCGTTACCAGTGCATTGCTGCCATCTGTGATGTGGTAAGCAACACCCTGACGGCAGAACCCAGCCGTTTCACCACTGCAGTAGATAAAATCGTGCTCAACCGTTTCCTCGATCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTgg                                                                                                                                                              >  1:220425/1‑243 (MQ=255)                                                                           ntGAAGCGTCGCAGCATCTGGATGCCGCCCTCGCCCGTCTGCGTAATGAGATGTACGATCCGGCGCTGCACATTGCCGATGCGCGTTACCAGTGCCTTGCTGCCATCTGTGATGTGGTAAGCAACACCCTGACGGCAGAACCCAGCCGTTTCACCACTGCAGTAGATAAAATCGTGCTCa                                                                                                                                                                                                                       >  1:60135/2‑180 (MQ=255)                                                                                                                      tANTGANANNGNNGATNCGGCGTTGTAAATTGCTGATTNNCGTGCTCCGNGCATTGCCGCCATCTGTGATGTGGTAAGTCACGCCCTGCTTGCAGACCCCAGCNGTGTCATCANNGCNGGAGGNANNNTNNNGCNCAACCGTTTCCTCGGTCTGNCGNNTTTCCTNTTTTTGATGTACCTGAGGTGCctgct                                                                                                                                                                <  2:121053/192‑1 (MQ=255)                                                                                                                                 aCGATCCGGCGCTGCACATTGCCGATGCGCGTTACCAGTGCATTGCTGCCATCTGTGATGTGGTAAGCAACACCCTGACGGCAGAACCCAGCCGTTTCACCACTGCAGTAGATAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGTGCATGGt                                                                                          <  1:23682/251‑1 (MQ=255)                                                                                                                                   gATCCGGCGCTGCACATTGACGATGCGCGTTACCAGTGCATTGCTGCCATCTGTGATGTGGTAAGCAACACCCTGACGGCAGAACCCAGCCGTTTCACCACTGCAGTAGATAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTTCAGCCGCTGTTTGACGTCGGCCCCGTGGCGCTATTTGTGCATGGTAt                                                                                        >  1:424629/1‑251 (MQ=255)                                                                                                                                      ccGGCGCTGCACATTGCCGATGCGCGTTACCAGTGCATTGCTGCCATCTGTGATGTGGTAAGCAACACCCTGACGGCAGAACCCAGCCGTTTCACCACTGCAGTAGATAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCGATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGAc                                                                                                                        <  1:36246/216‑1 (MQ=255)                                                                                                                                          cgcTGCTCATTGCCGATGCGCGTTACCATTGCATTGCTGCCATCTGTGATGTGGTAAGCAACACCCTGACGGCAGAACCCAGCCGTTTCACCACTACAGTAGATAAAATCGTGCTCAACCGTTTCCTCGGTCGGCCGAGTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCggg                                                                                                                                             <  1:86894/191‑1 (MQ=255)                                                                                                                                                acaTTGCCGATGCGCGTTACCAGTGCATTGCTGCCATCTGTGATGTGGTAAGCAACCCCCTGACGGCAGAACCCAGCCGTTTCACCACTGCAGTAGATAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGtt                                                                                                                                        <  1:86065/190‑1 (MQ=255)                                                                                                                                                                                       gggATGTGGTAAGCAACACCCTGACGGCAGAACCCAGCCGTTTCACCACTGCAGTAGATAAAATCGTGCTCAACCGTGTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTTCCTGATGTTCCTGCTGGCTATCAACATCGGCGGTGCGTTACAGCCGCTGTTTGTCGTCGGCTCCGTGGCGCTATTTGTGCAGGGTATTCAATg                                                                                  <  1:156778/203‑1 (MQ=255)                                                                                                                                                                                          aTGTGGTAAGCAACACCCTGACGGCAGAACCCAGCCGTTTCACCACTGCAGTAGATAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTGCCTCTTTTTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCggg                                                                                                                                             <  1:365727/143‑1 (MQ=255)                                                                                                                                                                                                                       cccAGCCGTTTCACCACTGCAGTAGATAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCTTGCTGGCTATCACCTTCGGCGGGGGGTTAAAGCCGCTGTTTGCCGTCGCCTCCTGGGCGCTTTTTTTGATTGGTTTTCAATGGATTGG‑TACACCCCCCCACTTCCCGGCCTGGCTGCCT‑TCTTTCCTCCCCCAGGCCCTgggggaaagggggaacaaacc  >  1:398595/1‑233 (MQ=255)                                                                                                                                                                                                                                    ccacTGCAGTAGATAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCAGCTGGCTATCAACATCGGCGGGGCGTTACCGCCGATGTTTGACGTGGGCTCCGTGGCGCTATTTGTGCATGGGATTCAATGGGTTGGCTACACGCTCC‑ACTTCCCGGACt                                                    >  1:168032/1‑189 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                         CATCCCGCTGAAACAACGTCGCTGGCTGGGCCTGCAAATGCTGGAAGGCGATATCTACAGCCGCGCCTACGCCGGTGAAGCGTCGCAGCATCTGGATGCCGCCCTCGCCCGTCTGCGTAATGAGATGGACGATCCGGCGCTGCACATTGCCGATGCGCGTTACCAGTGCATTGCTGCCATCTGTGATGTGGTAAGCAACACCCTGACGGCAGAACCCAGCCGTTTCACCACTGCGGTAGATAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGTGCATGGTATTCAATGGATTGGCTACACGCTCC‑ACTTCCCGGACTGGCTGACTATCTT‑CCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGT  >  NC_000913/3540991‑3541455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑