Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,341,309	G→A	P107L (CCG→CTG)	melR ←	DNA‑binding transcriptional dual regulator MelR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,341,309	0	G	A	100.0%	47.0 / NA	18	P107L (CCG→CTG)	melR	DNA‑binding transcriptional dual regulator MelR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/6);  total (12/6)

CGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGTGATTTGATCACCATGCCGTGAGTGACGTGGTTAATCAGGTCTTTATCCAGCGGCCAGGAGAGAAACAGATGCATCGGCAGATTAAAAATCGCCATGCTCTGACAGGTTCCGGTATCTGTTAGTTGGTGCGGTGTACAGGCCCAGAACAGCGTGATATGACCCTGATTGATATTCACTTTTTCATTGTTGATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCG  >  NC_000913/4341095‑4341530                                                                                                                                                                                                                       |                                                                                                                                                                                                                              cgAGGTTTTATTGACCAGAATCGGTTCACAGCCAGAGAGGCTAAATCGCTTGATCATCAGGCCAATTTCATCAATGGCGAGCTGACGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGTGATTTGATCACCATGCCGTGAGTGACGTGGTTAATCAGGTCTTTAACCAGCAGCCAGGAGAGAAACAGATGCATCGGCAGATTaaaa                                                                                                                                                                                            >  1:390736/1‑250 (MQ=255)                   aaTCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTGACTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCATTGATTTGATCACCATGCCGTGAGTGTCGTGGTTAATCTGGTCTTTATCCAGCAGCCAGGAGAGAAACAGATGCATCGGCAGATTAAAAATCGCCATGCTCTGACAgn                                                                                                                                                                         <  1:93953/251‑2 (MQ=255)                            ccagccagAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCAATGGAGAGCTGGCGAATTTGCTAGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGTGATTTGATCACCATGCCGTGAGTGACGTGGTTAATCAGGTCTTTATCCAGCAGCCAGGAGAGAAACAGATGCATCGGCAGATTACAAATCGCCATGCTCTGACAGGTTCCGGTAt                                                                                                                                                                >  1:360708/1‑251 (MQ=255)                                                                            ggCGANNTGNNNAANNTGCTCGTTCGGACTGTTTAATTCNTGCTGCCCGCGGCGCACTTCAAACGGGCTNANTNGTTGNNTGGNCAGTGCTTTGNTCTCCATGCCGTGAGTGACGTGGTTAATCAGGTCTTTATACAGCAGCCNGncgag                                                                                                                                                                                                                     >  2:380639/1‑150 (MQ=255)                                                                            ggCGAGCTTGCGAATGTGCTCGTTCGGACTGTTTAATTCCTGCTGCCTGCGGCGCATTTCAAACTGTCTAAGTTGCTTGGTGTTCAGTTATGTGATCACCTTGCCGTGAGTGACGTGTTAAATCAGGTCTTTATCCAGCATCCAGgagag                                                                                                                                                                                                                     <  1:380639/150‑1 (MQ=255)                                                                                              cTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGTGATTTGATCACCATGCCGTGAGTGACGTGGTTAATCAGGTCTTTATCCAGCAGCCAGGAGAGAAACAGATGCATCGGCAGATTaaaaa                                                                                                                                                                                           >  1:461264/1‑158 (MQ=255)                                                                                                           tttAATTCNTNNNGNNAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGTGATNTGATCACCATGCCGTNAGNGACGNGGTNAATCAGGTCTTTATCCAGCAGCCAGGAGAGAAACAGATGCATCGGCAGATTAAAAATCGCCATGCTCTGAc                                                                                                                                                                            >  2:349544/1‑160 (MQ=255)                                                                                                           tttAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGTGATGTGATCACCATGCCGTGAGTGACGTGGTTAAGCAGTTCTTTATCCAGCAGCCAGGAGAGAAACAGATGCATCGGCAGATTAAAAATCGCCATGCTCTGAc                                                                                                                                                                            <  1:349544/160‑1 (MQ=255)                                                                                                                                               ntAAGTTGCTGTGTGGCCAGTGATTTGATCACCATGCCGTGAGTGACGTGGTTAATCAGGTCTTTATCCAGCAGCCAGGAGAGAAACAGATGCATCGGCAGATTAAAAATCGCCATGCTCTGACAGGTTCCGGTATCTGTTAGTTGGTGCGGTGTACAGGCCCAGAACAGCg                                                                                                                            >  1:129673/2‑172 (MQ=255)                                                                                                                                                   gTTGCTGTGTGGCCAGTGATTTGATCACCATGCCGTGAGTGACGTGGTTAATCAGGTCTTTATCCAGCAGCCAGGAGAGAAACAGATGCATCGGCAGCTTAAAAATc                                                                                                                                                                                         >  1:161606/1‑107 (MQ=255)                                                                                                                                                         gtgtgGCCAGTGATTTGATCACCATGCCGTGAGTGACGTGGTTAATCAGGTCTTTATCCAGCAGCCAGGAGAGAAACAGATGCATCGGCAGATTAAAAATCGCCATGCTCTGACAGGTTCCGGTATCTGTTAGTTGGTGCGGTGTACAGGCCCAGAACAGCGTGATATGACCCTGATTGATATTCACTTTTTCATTGTTGATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGAcc                                    >  1:425866/1‑250 (MQ=255)                                                                                                                                                             ggCCAGTGATTTGATAACCATGCAGTGAGTGACGTGGTTAATCAGGTCTTTATCAAACAGCCAGGAAAGACACAGAGGCATCGGCAGATTAAAAATCGCCATGCTCTGACAGGTTCCGGTATCTGTTAGTTGGTGCGGTGTACAGGCCCAGAACAGCGTGATATGACCCTGATTGATATTCAATTTTTCATTGTTGATCAGGTATTCCCCATCGCCATCGCAAGGCCCATTCACTTCGACCTGACCAtacc                               >  1:356127/1‑248 (MQ=255)                                                                                                                                                                cAGTGATTTGATCACCATGCCGTGAGTGACGTGGTTAATCAGGTCTTTATCCAGCAGCCAGGAGAGAAACCGATGCATCGGCAGATTAAAAATCGCCATGCTCTGACAGGTTCCGGTATCTGTTAGTTGGTGCGGTGTACAGGCCCAGAACAGCGTGATATGACCCTGATTGATATTCACTTTTTCATTGTTGATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGt                            >  1:454585/1‑251 (MQ=255)                                                                                                                                                                cAGTGATTTGATCACCATGCCGTGAGTGACGTGGTTAATCAGGTCTTTATCCAGCAGCCAGGAGAGAAACAGATGCATCGGCAGATTAAAAATCGCCATGCTCTGACAGGTTCCGGTATCTGTTAGTTGGTGCGGTGTACAGGCCCAGAACAGCGTGATATGACCCTGATTGATATTCACTTTTTCAttg                                                                                         <  1:240337/190‑1 (MQ=255)                                                                                                                                                                              ccATGCCGTGAGTGACGTGGTTAATCAGGTCTTTATCCAGCAGCCAGGAGAGAAACAGATGCATCGGCAGATTACAAATAGCCATGCTCTGACAGGTTCCGGTATCTGTTGGCTGGTGCGGTGTCCAGGCCCAGAACAGCGTGATATGACCCTGATTGATATTCACTTTTTCATTGTTGATCAGGTATTCCACATCGCCATCGAAAgg                                                         >  1:360662/1‑208 (MQ=255)                                                                                                                                                                                           gaCGTGGTTAATCAGGTCTTTATCCAGCAGCCAGGAGAGAAACAGATGCATCGGCAGATTAAAAATCGCCATGCTCTGACAGGTTCCGGTATCTGTTAGTTGGTGCGGTGTACAGGCCCAGAACAGCGTGATATGACCCTGATTGATATTCACTTTTTCATTGTTGATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTgcg  <  1:7934/250‑1 (MQ=255)                                                                                                                                                                                                       cAGGTCTTTATCCAGCAGGCAGGAGAGAAACAGATGCATCGGCCGATTAAAAATAGCCATGCTCTGACTGGTTCCGGTATCTGTTAGTTGGTGCGGTGTACAGGCCAAGAACAGCGTGCTATGACCCTGATTGATATTCACTTTTTCAttgttg                                                                                      >  1:343813/1‑154 (MQ=255)                                                                                                                                                                                                       cAGGTCTTTATCCAGCAGCCAGGAGAGAAACAGATGCATCGGCAGATTAAAAATCGCCATGCTCTNACANGTTCNGGNATCTGTTAGTTGGTGNGGTGTACAGGCCCAGAACAGCGTGATATGACCCTGATTGATATTNNCNNNTNCAttgttg                                                                                      <  2:343813/154‑1 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                              CGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGTGATTTGATCACCATGCCGTGAGTGACGTGGTTAATCAGGTCTTTATCCAGCGGCCAGGAGAGAAACAGATGCATCGGCAGATTAAAAATCGCCATGCTCTGACAGGTTCCGGTATCTGTTAGTTGGTGCGGTGTACAGGCCCAGAACAGCGTGATATGACCCTGATTGATATTCACTTTTTCATTGTTGATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCG  >  NC_000913/4341095‑4341530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑