Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,025,288	C→T	A313V (GCT→GTT)	xanQ →	xanthine:H(+) symporter XanQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,025,288	0	C	T	100.0%	38.5 / NA	14	A313V (GCT→GTT)	xanQ	xanthine:H(+) symporter XanQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/8);  total (6/8)

GCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATGGGGTTATTCAGATGACTGGCGTCGCTTCACGTTATGTCGGGCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCAT  >  NC_000913/3025058‑3025507                                                                                                                                                                                                                                       |                                                                                                                                                                                                                            gcgcAATTTGCCTGTAATCACCACCCCACATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCGGGTGGTTGGCACGATTTATCTGCTTAGCGGGCTTGAAGCAGGCGGCGATATAACCTCCACGGTAATGGTTTGCCGCCGTCCCATTCAGGGAGAAGAGTATCAGTCCCGGGGGACAGGCGGCGGGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGTTGTCGGTTCATTACCATTaa                                                                                                                                                                                                         <  1:286434/251‑1 (MQ=255)                                      aaaTACGGCTTTATTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTGGCTTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGTTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATGGGGTTATTCAGATGAc                                                                                                                                                                    <  1:29230/251‑1 (MQ=255)                                            ggCTTTGGTTTTCGCTTCCATCAGTTCCGGGGGTTTGGCACGTTTAATCTGCTAAGCGGGCTGGAAGCAGTGGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGTTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATGGGGTTATTCAGAGGACTGGCGt                                                                                                                                                              <  1:28636/251‑1 (MQ=255)                                                                                  cACGATGTATCTGCTTAGCGTGTGGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGTTGTCGGTTCATTACCATTAACCACGTGTGCGCAAAATAATGGGTTTATTCAGATGACTGGCGTCGCTTCACGTTATGTCGGGCGAACCATCGCGGTAATGc                                                                                                                        <  1:459644/251‑1 (MQ=255)                                                                                               cTTAGCGGGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGTTGTCGGTTCATTACCATTAACCACGTTTg                                                                                                                                                                                                <  1:72824/166‑1 (MQ=255)                                                                                                    cGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGTTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATGGGGTTATTCAGATGACTGGCGTCGCTTCAc                                                                                                                                                      >  1:388095/1‑203 (MQ=255)                                                                                                         tGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGTTGTc                                                                                                                                                                                                                         >  1:1054/1‑131 (MQ=255)                                                                                                                                           ggTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGTTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATGGGGTTATTCAGATGACTGGCGTCGCTTCACGTTATGTCGGGCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCgg                                                               <  1:138133/251‑1 (MQ=255)                                                                                                                                                cccGCCGCCCAATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGTTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATGGGGTTAGTCAGATGACTGGCGTCGCTTCACGTTATGTCGGGCGAACCAGCGCGGTAATGCTGGTTAGCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGt                                                           <  1:70228/250‑1 (MQ=255)                                                                                                                                                                                                        ncAGATGGTCTGGTTTCTGTTATCGCCTCCGTTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATGGGGTTATTCAGATGACTGGCGTCGCTTCACGTTATGTCGGGCGAACCATCGCGGGAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGcat  >  1:406799/2‑251 (MQ=255)                                                                                                                                                                                                        gCAGATGGTCTGGTTTCTGTTATCGCCTCCGTTGTCGGTCCTTTACCATTAACCACGTTTGCGCAAAATAATGGGGTTATTCAGATGCCTGGCGTCGCTTCACGTTATGTCGGGCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGGAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGcat  >  1:341098/1‑251 (MQ=255)                                                                                                                                                                                                         cAGATGGTCTGGTTTCTGTTATCGCCTCCGTTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATGGGGTTATTc                                                                                                                                                                           <  1:32889/81‑1 (MQ=255)                                                                                                                                                                                                         cAGATGGTCTGGTTTCTGTTATCGCCTCCGTTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATGGGGTTATTc                                                                                                                                                                           >  2:32889/1‑81 (MQ=255)                                                                                                                                                                                                                   ggTTTCTGTTATCGCCTCCGTTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATGGGGTTATTCAGATGACTGGCGTCGCTTCACGTTATGTCGGGCGAACCATCGCGGTAATGCTGGTTATcc                                                                                                               >  1:18518/1‑131 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                            GCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATGGGGTTATTCAGATGACTGGCGTCGCTTCACGTTATGTCGGGCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCAT  >  NC_000913/3025058‑3025507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑