| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,643,679 | A→T | L209Q (CTG→CAG) | ydfU ← | protein YdfU |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,643,679 | 0 | A | T | 97.0% | 90.2 / ‑6.3 | 34 | L209Q (CTG→CAG) | ydfU | protein YdfU |
| Reads supporting (aligned to +/- strand): ref base A (1/0); major base T (18/14); minor base G (1/0); total (20/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
CGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGATTTTGTTG > NC_000913/1643447‑1643893 | cGGATCGACGGCTGGCATCCCGCAGAAAGCACACGGCTGTGTCTTAACACAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGGGCGTTGTTTCAATATGCAGGTGGCGGTCTGCTCTCCCGGTACGATGt > 1:139087/1‑251 (MQ=255) gtcgGCTGGCATACCGCAGCAAGCACACGGCTGGGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGGCTTGTCCTTTGGTGATTTTTGTTGCTGGTGGGCGTCAGGCAACGGTGCAAGATTTTGTGTACGTTGTTGCAATATTCTGGTGGCGGTCTGCTCTCCCGGTACGATg < 1:192728/244‑1 (MQ=255) gcaagcaCACGGCTGTGGCTTAACCCACCGTGTGTATTTATCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGCCTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCtctc > 1:10132/1‑215 (MQ=255) gtgtCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGCGGGCGTAAGGCAACGGTGCAAGATTGTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTg < 1:461226/249‑1 (MQ=255) gtCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCCTGAAAGATTTCGGCCACTCCGGATCAGCGGCAATGCTTACCACCGTCATTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGATGGCGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGCGCAGATTTTTTCCGCTGGTa > 1:288290/1‑250 (MQ=255) nggcgACGTTTAGGCAGCTTCATGAAAGATTTCGGAGACTATGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTACGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCACAGCGAac > 1:320588/2‑226 (MQ=255) ggcgANNTTNNNGANNCTTCATGAACGATTTCGGAGACTNTGGATCAACGGTGATGCTTAACAACGTCTNTNCNTGTGNNGCTNTTTGTTGCTGNTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTCGTTTCAAAATGNTNGTGGCGGTAAGCACTCCCGGTCCGATGTCGCNATCGCGGTACACCGGGCGGCTTTTNTCCTNNGGNannnnn > 2:379788/1‑213 (MQ=255) ggcgACGGTTAGGCAGCTGCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGTCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTGTCAATATTCTGGTGGCGGTCTTCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCg < 1:379788/222‑1 (MQ=255) tCATGAAAGATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGc < 1:246184/249‑1 (MQ=255) ngATTTCGGAGACTCTGGATCAACGGCGATGCTTACCACAGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGt > 1:99507/2‑161 (MQ=255) gATTTCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGGGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAg > 1:109844/1‑168 (MQ=255) gATTTCGGAGACTCNNGATCAACGGNGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGNCAACGGGGCNNGANTTTTGNTNNNTNNNTTNAATATGCTGGTGGCGGTCTNCTNNCCCGGTNCGATGTCGCTTTCGCGGTACACCGAg < 2:109844/168‑1 (MQ=255) ggATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGa > 1:444227/1‑182 (MQ=255) gATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGCTTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCCATATGCTGGTGGCGGTCTGCTCTCCCGGTACg > 1:177598/1‑128 (MQ=255) aaCGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCaa > 1:138741/1‑249 (MQ=255) aaCGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCAGGTGGGCGGAAAGCAACGGTGCAATAGTGTTTGTGCTTTGGTTCAATATGCTGGTGGCGGTCTTCTCTCCCGGTACGATGGCGCGTGCGCGGTACACCGAGCAGATGGTTGCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTc < 1:503581/195‑1 (MQ=255) ggTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTACGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGt > 1:67280/1‑137 (MQ=255) gtgATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTcg < 1:372682/104‑1 (MQ=255) gtgATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAac > 1:12434/1‑147 (MQ=255) gtgATNNTTNNNGCNNGGGGGCGTAAGGCAACGGTGCAANATTTTTTGTGCGTTGTTTCAATATGCTGGNGNCNGTCTNNTCTNCCGGTACGATNTCGCTTTcg > 2:372682/1‑104 (MQ=255) gttgCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACg < 1:78545/83‑1 (MQ=255) gttgCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGc < 1:383658/121‑1 (MQ=255) gttgCNNGTNNNCGNNAGGCAACGGTGCAAGCTTTTTTGNGCGTTGTTTCAATATGCTGGTGGCGGTCTNCNCNCCCGNNACGNTGTCGCGTTTNCGGTACACCGGGCAGATTTTTTCCGc > 2:383658/1‑121 (MQ=255) cTGGTGGGCGTAAGGCAGCTGGGCAAGATTTTGTGTGCTTTGTTTCAATATGCTGGCGGCTTTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTc < 1:244207/114‑1 (MQ=255) gtggGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCa < 1:470185/174‑1 (MQ=255) gCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCgg < 2:10441/87‑1 (MQ=255) gCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCgg > 1:10441/1‑87 (MQ=255) gCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATACTTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGAAGGATGACGTCAATCAc > 1:1034/1‑251 (MQ=255) ngCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGACCGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGAt > 1:44739/2‑160 (MQ=255) ggCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGCACGATGTAGCTTTCGCGGTCCAACGAGCAGATTTTTTCAGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGCCCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGc > 1:256062/1‑251 (MQ=255) gCAACGGTGCAAGATTTTTTGTGCGGTGTTTCAATATGCTGGTGTCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGt < 1:85220/140‑1 (MQ=255) tgttTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCTCGGTACACCGAGCATATTTTTTCAGCTGGTAATCCCAGCGAac < 2:320588/94‑1 (MQ=255) gttTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCCTCCACCACCTGATTGCAGCCCGCCCACCAGGCTAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGCCAATCACCCATGCTGTCAGCTTttgttg > 1:466644/1‑238 (MQ=255) ttCAATATGCTGGTGGCGGTCGGCTCTCCCGGTACGATGTCGCTTTATCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGAAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTa > 1:20725/1‑183 (MQ=255) | CGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGATTTTGTTG > NC_000913/1643447‑1643893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |