Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,766,540	G→A	R208H (CGC→CAC)	rnlA →	RNase LS, toxin of the RnlAB toxin‑antitoxin system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,766,540	0	G	A	92.9%	31.6 / ‑3.5	14	R208H (CGC→CAC)	rnlA	RNase LS, toxin of the RnlAB toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (6/7);  total (7/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

CCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTTGGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCGCACTTACTTGCAGACTGTAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCCCCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAGGTGTCTTAAAAAGTAAGATGAGTGGGTTAGGCATGCCAGTACAGCAGCCGGCAGGTTTTGGAACTTACTTTGATAAACCTGC  >  NC_000913/2766376‑2766774                                                                                                                                                                     |                                                                                                                                                                                                                                           ccTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTTGGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCACACTTACTTGCAGACTGTAATGGCCGATGCTTACCCGCATCTCCACGTGACTGc                                                                                                                                                                                      <  1:25549/219‑1 (MQ=255) ccTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTTGGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCACACTTACTTGCAGACTGTAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTAtg                                                                                                                                                           >  1:152734/1‑246 (MQ=255)   ngCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTAACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTTGGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCACACTTACTTGCAGACTGTAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTg                                                                                                                                                                            >  1:54212/2‑227 (MQ=255)                      ncTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGAAGCCCTGCTTGATTTACAGGGTTTGGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCGCACTTACTTGCAGACTGTAAACGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAAGGCTATGTGTTAAACTCGCCGCCCCTGATTTGc                                                                                                                                 >  1:99764/2‑251 (MQ=255)                      gCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTTGGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCACACTTACTTGCAGACTGTAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCCCCTGATTTGc                                                                                                                                 >  1:396675/1‑251 (MQ=255)                       cTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTTGGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCACACTTACTTGCAGACTGTAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCt                                                                                                                                                                          >  1:194346/1‑209 (MQ=255)                                    gCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTTGGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCACACTTACTTGCAGACTGTAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCCCCTGATTTGCCTGACTACTGTATg                                                                                                                   >  1:353037/1‑251 (MQ=255)                                                                     ttAACTTGGCAGCCCTGCTTGATTTACAGGGGTTGGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCACACTTACTTGCAGACTGTAATGTCCTATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTCTAAAACTCGCCGCCCCTGATTTGCCTGACGACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAgg                                                                                   <  1:359699/250‑1 (MQ=255)                                                                          ttGGCAGCCCTGCTTGATTTACAGGGTTTGGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCACACTTACTTGCAGACTGTAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAg                                                                                                                                                                  <  1:258/166‑1 (MQ=255)                                                                                   cTGCTTGATTTACAGGGTTTGGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGGTGCCCACACTTACTTTCAGACTGTAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTcn                                                                                                                                                                        <  1:95463/151‑2 (MQ=255)                                                                                                          gaAAGTGCTAATCCGCCAGGAGGATGGTATAGCTAATATTGTTCAACAGGAGGTTGCCCACACTTACTTGCAGACTGTAATGGCCGATNNTTAccc                                                                                                                                                                                                        >  2:18811/1‑96 (MQ=255)                                                                                                             aGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCACACTTACGTGCAGACTGTAAGGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCCCCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAGGTGTCTTAAAAAGTAAGATGAGTGGGTTAGGCATGCCAGTAc                                          <  1:18811/251‑1 (MQ=255)                                                                                                                                                     cAACAGGAGTGTGCCCACACTTACTTGGAGACTGTAATGGCCGATGCTTACCCGCATCTTCACGTTACTGCCGAAAAATTGCGCGTTTCAGGGCTATGTGTTAAACTCGCCGCCCCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACGACGCACCATTGAAGGTGTCTTAAAAAGTAAGATGAGTGGGTTAGGCATGCCAGTACAGCTGCCGGCAGGTGTTGGAACTTACTTGGATAAACctgc  <  1:391900/251‑1 (MQ=255)                                                                                                                                                          ggaggTTGCCCACACTTACTTGCAGACTGTAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCCCCTGATTTGc                                                                                                                                 <  1:141388/119‑1 (MQ=255)                                                                                                                                                                     |                                                                                                                                                                                                                                           CCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTTGGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCGCACTTACTTGCAGACTGTAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCCCCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAGGTGTCTTAAAAAGTAAGATGAGTGGGTTAGGCATGCCAGTACAGCAGCCGGCAGGTTTTGGAACTTACTTTGATAAACCTGC  >  NC_000913/2766376‑2766774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑