| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,366,277 | G→A | G243D (GGC→GAC) | puuE → | 4‑aminobutyrate aminotransferase PuuE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,366,277 | 0 | G | A | 90.0% | 14.2 / ‑5.9 | 10 | G243D (GGC→GAC) | puuE | 4‑aminobutyrate aminotransferase PuuE |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (5/4); minor base C (0/1); total (5/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTTCGGTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAACACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAATCAGACATCGAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTGATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTGCTCAACATTATCGACAAAGAATCACTCTG > NC_000913/1366036‑1366508 | ggTTCGGTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAACACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAATCAGACATCGAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGGTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTGATGAAGTGCAAAGCGACTTTGcgcg < 1:248712/251‑1 (MQ=255) gATTTACACGGCATTTCAACACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAATCAGACATTGAAGCGAAGCAGGTGGCGGCGATTATTTTCGACCAGGTGCAGGGCGCGGGCGGTTTCAACGTTGCGCCAAAAGGGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTGATGAAGTGCAAAGCGACTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCAtt > 1:467796/1‑250 (MQ=255) cATCGAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTGATGAAGTGCAAAGCGACTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGa > 1:474277/1‑221 (MQ=255) cgATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGAAGCGCACGGTATTGCGATGATTGCTGATGAAGTGCAAAGCGACTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAAtatt > 1:289396/1‑251 (MQ=255) agggcgagggcgGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTGATGAAGTGCAAGGCGACTTGGCTCTTTCCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGAGTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGc < 1:252286/198‑1 (MQ=255) gCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTGATGAAGTGCAAGGCGACTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATACGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGCTGCCGCTTTCGGGCGTGTTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTgg > 1:464554/1‑215 (MQ=255) ttCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTGATGAAGTGCAAAGCGACTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCgg < 1:260902/159‑1 (MQ=255) gCTGATGAAGTGCAAAGCGACTTTGCGCGTACCTGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGGGGCTGCCGCGCACGCGGTGCTCAACATTATCGACAAAGAATCACTCTg < 1:330391/251‑1 (MQ=255) tGCAAAGCGACTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCAGGATTTAATGAAGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTaa > 1:64582/1‑124 (MQ=255) cAAAGCGCCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGAGGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGACACCTACGCCGGTAACCCGCTGGCGGTGGCTGCcgc < 1:363576/200‑1 (MQ=255) | GGTTCGGTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAACACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAATCAGACATCGAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTGATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTGCTCAACATTATCGACAAAGAATCACTCTG > NC_000913/1366036‑1366508 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |