Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,394,231	G→A	A56T (GCG→ACG)	nnr →	NAD(P)HX epimerase/NAD(P)HX dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,394,231	0	G	A	100.0%	21.6 / NA	10	A56T (GCG→ACG)	nnr	NAD(P)HX epimerase/NAD(P)HX dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/4);  total (6/4)

CAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGAGGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGATGTGCTGGGGCTCACACTCTATGAGCTGATGCTTCGCGCTGGCGAGGCCGCATTCCAGGTGTGTCGTTCGGCGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACGCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGT  >  NC_000913/4394011‑4394454                                                                                                                                                                                                                             |                                                                                                                                                                                                                                naGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGCTCGAGGGGCTCTGACATGACGGACCATACAATGACGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGATGTGCTGGGGCTCACACTCTATGAGCTGATGCTTCGCGCTGGCGAGGCCGCATTCCAGGTGTGTCGTTCGACGTATCCTGACGCCCGCCACTGGCTGGTGc                                                                                                                                                                                                   >  1:102102/2‑251 (MQ=255)             tttAATTTTTTGCGCTAACTGATTGAGATCGAGGGGCTCTGACAGGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGATGTGCTGGGGCTCACACTCTATGAGCTGATGCTTCGCGCTGGCGAGGCCGCATTCCAGGTGTGTCGTTCGACGTATCCTGAng                                                                                                                                                                                                                     <  1:394513/221‑1 (MQ=255)                         cgcTAACTGATTGAGATCGAGGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGATGTGCTGGGGCTCACACTCTATGAGCTGATGCTTCGCGCTGGCGAGGCCGCATTCCAGGTGTGTCGTTCGACGTATcc                                                                                                                                                                                                                          <  1:326191/204‑1 (MQ=255)                             aaCTGATTGAGATCGAGGGGCTCTGAAATGCCGGACCATACAATGAAGAAAACCCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGATGTGCTGGGGCTCACACTCTATGAGCTGATGCTTCGCGCTGGCGAGGCCGCATTCCAGGTGTGTCGTTCGACGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGgcggcg                                                                                                                                                                        <  1:26764/250‑1 (MQ=255)                                                            cGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGATGTGCTGGGGCTCACACTCTATGAGCTGATGCTTCGCGCTGGCGAGGCCGCATTCCAGGTGTGTCGTTAGACGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGGCATGGTAATAACGGCGGCGATGGCTACGTGGTCGCGCGACTGGCCaaaacg                                                                                                                                        >  1:370289/1‑248 (MQ=255)                                                                                 aCCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGCACGCGAGGCGGCAGATGTGCTGGGGCTCACACTCTATGAGCTGATGCTTCGCGCTGGCGAGGCCGCATTCCCGGTGTGTCGTTCGACGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAAc                                                                                                                                                                               >  1:120377/1‑191 (MQ=255)                                                                                                           tGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGATGTGCTGGGGCTCACACTCTATGAGCTGTTGCTTCGCGCGGGCGAGGCCGCATTCCAGGTGTGTCGTTCGACGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGTCGCGCGACTgg                                                                                                                                                <  1:416708/196‑1 (MQ=255)                                                                                                                                                                                         cgcgCTGGCGAGGCCGCATTCCAGGTGTGTCGTTCGACGGATCCTGACGCACGCCACTGGCTGGTGCTGTGCGGTCCTGGTAATAACGGCGGCGCTGGCTACGTGGTCGCGCGACGGGCCAAAAAGGTCGGCATTGa                                                                                                                             >  1:252528/1‑137 (MQ=255)                                                                                                                                                                                                     gCCGCATTCAACGTGTGTCGTTCGACGACTCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGCTGGCTACGTGGTCGCGCGACTGGCCACAGCGGTCGGCATTGAGGCCACGTTGTTGGCCCCGGCGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGACGCATGGGTAAACGCGGGTGGCGAGATCCCTGCTTCGAATATTGTCTGGCCCg         >  1:158384/1‑241 (MQ=255)                                                                                                                                                                                                      ncGCATTCCAGGTGTGTCGTTCGACGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACGCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGt  >  1:123044/2‑247 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                CAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGAGGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGATGTGCTGGGGCTCACACTCTATGAGCTGATGCTTCGCGCTGGCGAGGCCGCATTCCAGGTGTGTCGTTCGGCGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACGCGGGTGGCGAGATCCATGCTTCGAATATTGTCTGGCCCGAATCGGT  >  NC_000913/4394011‑4394454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑