Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	612,410	G→A	P29S (CCT→TCT)	fepA ←	ferric enterobactin outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	612,410	0	G	A	100.0%	30.6 / NA	15	P29S (CCT→TCT)	fepA	ferric enterobactin outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/7);  total (8/7)

CAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTGACCACTGGTGGAGTTACCGGTCAGGTTAACGCCTGGCATGGTACGGATGATCTTCGACACATCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATC  >  NC_000913/612170‑612586                                                                                                                                                                                                                                                 |                                                                                                                                                                                 caatcaaAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTGACCACTGGTGGAGTTACCGGTCACGTTAACGCCTGGCATGGTACGGATGATCTTCGACACATCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAAACGCCCGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGAAGTAtcggtc                                                                                                                                                                        >  1:471368/1‑251 (MQ=255)                      ccGGACCCATATCGCGAATATCAATCTGGCGGTGATTCCCACGGTGACCACTGGTGGAGTTTCCGGTCAGGTTAAGGCCTGGCAGGGTACGGATGATCTTCGACACATCGCGGGCATGCTGGTTTTTGCTGATTTCATCTGCGGGTATTGTCGAAACGCCAGGCGCCTGGAGGGTCTGCTCGGCGGCGTTATCGACAATAGGATCGTCATGTGAAAGTGAAGTATCGGTCGGCTCTTGTGCCTGCGCTAcc                                                                                                                                                   <  1:218203/251‑1 (MQ=255)                                                             cACGCTGACCACTGGTGGAGTTACCGGTCAGGTTAACGCCTGGCATGGTACGGATGATCTTCGACACTTCGCGGGCAACCGGGGTTTTGCGGATTTCATCTGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGGTCTGCTCGGCGGCGGTAACGGCAATAGTATCGTCATGTGAAACAAAAGTAGCGGTCTGCGTTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGCCCAACAAGGCCAGGgaatga                                                                                                            <  1:286889/251‑1 (MQ=255)                                                             cACGCTGACCACTGGTGGAGTTACCGGTCAGGTTAACGCCTGGCATGGTACGGATGATCTTCGACACATCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGAAGTAtcg                                                                                                                                                                           >  1:418627/1‑188 (MQ=255)                                                                                       gTCAGGATAACGCCTGGCATGGTAAGGATGATATTAGACACATCGCGGCCCACCGGGTTTTTGCGGATTTCATCTGCGGCGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGAGGCGGTAACGACAATAGTATCGTCATGTGAAACAGAAGTATCGGGCGGCTCTTGTGCCTGCGCTCCCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCt                                                                                  >  1:423068/1‑251 (MQ=255)                                                                                                                                aTCGCTGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCCCTGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGAAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCACCCAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGtca                                         <  1:193385/251‑1 (MQ=255)                                                                                                                                aTCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGAAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGgtcgtc                                          >  1:180348/1‑250 (MQ=255)                                                                                                                                      ggCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGAAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCaa                                                            >  1:29705/1‑226 (MQ=255)                                                                                                                                                                   cGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGAAGTAGCGGTCGGCTCTtgtg                                                                                                                                                              <  1:184458/99‑1 (MQ=255)                                                                                                                                                                       gATGGTCGAAACTCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGAAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTTTTTTATTCCTGCATTTGTGCCACGAATGGCAACTGTCGTGCATGGTCGGCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATc  <  1:455223/251‑1 (MQ=255)                                                                                                                                                                            tCGAAACGCAAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGAAGTATCGGTCGGCTCTtgtg                                                                                                                                                              >  1:400359/1‑90 (MQ=255)                                                                                                                                                                             cGAAACGCCAGGCGCCTGTAAGTCCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGAAGTATCGGTCGGc                                                                                                                                                                     <  1:449347/82‑1 (MQ=255)                                                                                                                                                                                          gcCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGAAGTATAGGTGGGCTCTTGTGca                                                                                                                                                            >  1:288330/1‑77 (MQ=255)                                                                                                                                                                                           ccTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGCCATGTGAAACAGAAGTATCGGTCGGCTCTTGAGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGCCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCAttttt                                                                          >  1:218301/1‑159 (MQ=255)                                                                                                                                                                                                                  gTAATGACAATAGTATCGTCATGTGAAACAGAAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCTGCTACCGCGAAAACCTTTg     <  1:171533/205‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                 CAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTGACCACTGGTGGAGTTACCGGTCAGGTTAACGCCTGGCATGGTACGGATGATCTTCGACACATCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATC  >  NC_000913/612170‑612586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑