Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,646,753 G→A S191S (TCC→TCT)  pbpC ← peptidoglycan glycosyltransferase PbpC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,646,7530GA94.7% 49.9 / ‑5.7 19S191S (TCC→TCT) pbpCpeptidoglycan glycosyltransferase PbpC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (10/8);  minor base T (1/0);  total (11/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATCCCCTGCAACGTACCGCCAAACGGAGCGCGGTTAAGATACAAGGTCAGAATTTCACGCTTAGACAGATGCCATTCCAGTTGCAACGCGCGCCAGAGCTGGCGAATTTTGCCGCCAAATGTTTTGGGGTGAGGATCAAGCAGACGAGCAACCTGCATAGTG  >  NC_000913/2646532‑2646969
                                                                                                                                                                                                                             |                                                                                                                                                                                                                        
ccGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTCCCGGCTCACGGGACCACACCCCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCGAAGCCGGCTGGGCGTTTGCGGCAAAACCGCCAGCATTGCCGCCTCAGAATAGCTTAAATTCGCAGGCGATTTTcc                                                                                                                                                                                             <  1:356383/251‑1 (MQ=255)
        catGCCCGAAACCAGCGGTGCCATTTGCGGCATTTGGCGGGGGTCCAGCCAGATGGGTTCTTCCCTTGTTTCTTTTACCTGCTCACGGAACCACACACCTGGCACGGCCATCCGTTCGAGCCCTTTATTCCTCGCTGCTTCGGCCCGCTCCGGCCAACGATCCGGGCGAAGACGGCGGGGCGCTGGCGGCAAACCCGCCATCATTGCCGCCGCAGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATaan                                                                                                                                                                                     <  1:97591/251‑2 (MQ=255)
                     aGCGGGGCCAGTTGCGGCATTTGTCGGGGGGCCAGCAAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACCCCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTGCGGCACGCTCCGGCCAACGAGCCGGGCGAAGACGGGTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCAGAATAGCTTAAATTCGCAGGCGATGTTCCGAGATAAGCCCAACTTGCCGc                                                                                                                                                                        <  1:138539/251‑1 (MQ=255)
                                                               ggTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACTCCTTGCACGGCAATGCGTTCGATCACTTTATTACGCGCGGCTTCCGAACGCACCGGCCACGGAACCGAGCCAAGGAGGCTAGGCACTTACGCCAAAACCGCCCGCATTGCCGCCTCTGAATAGATTCAATTCGCCGGCGATTTTCCGCGATAAGCCCAAATTCCCGCACCGATCCCATGCAACGTACCCCCAAACGGAGCGCGCTTa                                                                                                                                >  1:235438/1‑249 (MQ=255)
                                                                  ncttcCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCAGTTCGAGCACTTTATTACGCGCGGCTTCGGCAAGCTCCGGCCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCAGAATAGCTTAAATTCGCAGGCGATTTTCCgag                                                                                                                                                                                          >  1:315156/2‑188 (MQ=255)
                                                                                           cACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGCCACGCTCCGGCCAACGATCCGGGCGCAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCAGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATCCCCTGCAACGTACCGCCAAACGGAGCGCGGTTAAGATACAAGGTCAGAATTTCACGCTTAGAc                                                                                                  >  1:203087/1‑251 (MQ=255)
                                                                                           cACGGGACCACACACCTTGCACGGCCATCCGTTCGAGAACTTTATTACGCCCGCCTTCGGCACGCTCCGGCCAACGATCCGGGCCAAGCCGCCTGGGCGCATGCCGCAAACCCGCCCGCATTGCAGCCTCAGAATAGCTTAAATTCGCAGGc                                                                                                                                                                                                     >  1:462263/1‑152 (MQ=255)
                                                                                                            nnccnnnnnaccntttCGAGCCCTTTATACCGCGGGGTTTCGGCCCGCTCCGGCAAACGATCCGGGCGAAGACGGCGGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCAGAATAGCTTAAATTCGCAGGCGATTTTCCgag                                                                                                                                                                                          <  2:315156/132‑1 (MQ=255)
                                                                                                              cagggCCATCCGTTCGAGCACTTTATTCCCCTCGGCTTCGGCACGCCCCGGCCAACGATCCGGGCGAAGACGGTTGGTCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCAGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCa                                                                                                                                                                       <  1:1924/160‑1 (MQ=255)
                                                                                                              cACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCACCGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCAGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATCAGCCCAACTTGCCGCACCGATCCCCTGCAACGTACCGCCCAACGGAGCGCGGTTAAGATACCAGGTCAGAATTTCACGCTTAGACAGATGCCATTCCAGTTGc                                                                                >  1:455791/1‑250 (MQ=255)
                                                                                                                 ggCCATCCGTTCGAGCCCTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCAGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATCCCCTGCAACGTACCGCCAAACGGAGCGCTGTTAAGATACAAGGTCAGAATTTCACGCTTAGACAGATGCCATTCCAGTTGCaaag                                                                            <  1:481330/251‑3 (MQ=255)
                                                                                                                  gCCATCCTTTCGAGCACTTTATTACGCGCGGCTCCGGCCCGCTCCGGCCAACGATCCGGGCAAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCAGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCTCACCGATCCCCTTCAACGTACCGCCAAACGGAGCGCGGTTAAGATACAAg                                                                                                                       <  1:437400/207‑1 (MQ=255)
                                                                                                                                          ngcgcgGCTTCGGCACGCTACGGCCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCAGAATAGCTTAAATTCGCAGGCGCTTTTCCGAGATAAGCCCa                                                                                                                                                                                 >  1:104135/2‑125 (MQ=255)
                                                                                                                                                                                            cgcTTGCGGCAAAACCGCCAGCATTGCCGCCTCAGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCAcc                                                                                                                                                                     >  1:263085/1‑87 (MQ=255)
                                                                                                                                                                                             gnTTGCGGCAAAACCGCCAGCATTGCCGCCTCAGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATCCCCTGCAACGTACCGCCAAACGGAGCGCGGTTAAGATACAAGGTCAGAATTTCACGCTTAGACAGATGCCATTCCAGTTGCAACGCGCGCCAGAGCTGGCGAATTTTGCCGCCAAATGTTTTGGGGTGAGGATCAAGCAGACGAGCAACCTGCATAGTg  >  1:394841/1‑249 (MQ=255)
                                                                                                                                                                                              cTTGCGGCAAAACCGCCAGCATTGCCGCCTCAGAATAGCTTAAATTCGCAGGCGATTTTCCGAGCTACGCCCAACTTGCCGCACCGATCCCCTGCACCGTACCGCCAAACGGAGCGCGGTTCAGATACTAGGTCAGAATTTCACTCTTAGACAGATg                                                                                             >  1:288979/1‑157 (MQ=255)
                                                                                                                                                                                                 gCGGCAAAACCGCCAGCATTGCCGCCTCAGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAATTTGCCGCACCGATCCCCTGCAAAGTACCGCCAAACGgcgcgc                                                                                                                                     >  1:452183/1‑114 (MQ=255)
                                                                                                                                                                                                   ggCAANNCCNNNAGNNTTACCGCCTCAGAATAGCTTAAANTCGCAGGCGGTTTTCCGAGATAAGCCCAANTNGNCGCANNGATNCCCTGCAACGNACCGCCAAACGGAGCGAGCTTAAGACAACAGGTCAGAATTTCACGCTTNGNCAGATGCCATTCCAGTTGTAACGCGCGCCAGNGCTGGCGAATTTTgc                                                    >  2:370201/1‑193 (MQ=255)
                                                                                                                                                                                                                gCATTGCCGCCTCAGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATCCCCTGCAACGTACCGCCAAACGGAGCGCGGTTAAGATACAAGGTCAGAATTTCACGCTTAGACAGATGCCATGCCAGTTGCAACGCGCGCCAGAGCTGGCGAATTTTGCCGCCAAATGTTTTg                                      <  1:214279/194‑1 (MQ=255)
                                                                                                                                                                                                                             |                                                                                                                                                                                                                        
CCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATCCCCTGCAACGTACCGCCAAACGGAGCGCGGTTAAGATACAAGGTCAGAATTTCACGCTTAGACAGATGCCATTCCAGTTGCAACGCGCGCCAGAGCTGGCGAATTTTGCCGCCAAATGTTTTGGGGTGAGGATCAAGCAGACGAGCAACCTGCATAGTG  >  NC_000913/2646532‑2646969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑