Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	460,576	G→A	K563K (AAG→AAA)	lon →	Lon protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	460,576	0	G	A	100.0%	10.9 / NA	7	K563K (AAG→AAA)	lon	Lon protease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/3);  total (4/3)

GGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAGTCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATG  >  NC_000913/460333‑460789                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                      ggATCGTATGGAAGTGATTCGCCTCTCCGGATATACCGCAGCTGAAAAACAGCCCATCGCCAAACGTCCCCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACGGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTa                                                                                                                                                                                                                 >  1:243536/1‑250 (MQ=255)                                                                                                              ggTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACg                                                                                                                                  <  1:41354/220‑1 (MQ=255)                                                                                                                                                       tCGTTACTACACCCGTGAGGCGGGCGAGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGTGGTGACTTGCTGACCATTGAAAcc                                                           <  1:153398/250‑1 (MQ=255)                                                                                                                                                                              gcgtgcgtGGGCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCAGTAAAACATATCGAAATTAACGGCGATAACCTGCATGan                                                                                                                                                                             <  1:99244/113‑2 (MQ=255)                                                                                                                                                                                                gTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGAGTGGACGGAAGTGGGCGGTGACTTg                                                                          >  1:481785/1‑194 (MQ=255)                                                                                                                                                                                                               tgtgTCGCAAAGCGGTTAAGCAGTTACTGCTAGATAAATCATTAAAACATATCGCCATTAAAGGCGATAACCTGCATGAATATATAGGTGTTCAGCGATTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGGAACCGGTCTGGCGTGGACGGAAGTGGGCGGGGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTgctg  >  1:452958/1‑248 (MQ=255)                                                                                                                                                                                                                            ggTTAAGCAATTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGAGATAACCTGCATCACTATCTCGGTGTTCAGCGTTTAGACTATGGCCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCAGGCGTAGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAAcc                                                           >  1:295360/1‑181 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                      GGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAGTCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATG  >  NC_000913/460333‑460789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑