Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	873,115	G→A	R46H (CGC→CAC)	pdeI →	putative c‑di‑GMP phosphodiesterase PdeI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	873,115	0	G	A	100.0%	30.9 / NA	12	R46H (CGC→CAC)	pdeI	putative c‑di‑GMP phosphodiesterase PdeI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/6);  total (6/6)

TCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTCACTGTATTTATTAAAAAATACAGAGGAGATTCAACGCGCCGTGATTCTTAAAATGGAAATGATGCCATTTTTAGACTCGGTGGGACTGGTACTTGATGATAATAAATATTATCTTTTTTCGCGGAGGGCGAAT  >  NC_000913/872874‑873323                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                 tCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATAAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATGGGCAGCACTGTCATGTATGGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCACTTTGAAc                                                                                                                                                                                                          <  1:502128/250‑1 (MQ=255)                              ngTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGCATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCACtt                                                                                                                                                                                                               >  1:149348/2‑216 (MQ=255)                                   aaaaGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGCAAAGATAAAGATACGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTCTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCACTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTaa                                                                                                                                                                           >  1:494769/1‑247 (MQ=255)                                                     cAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCGCGGTCATTTATTGGTCTTTTTGTCATCATTAACGATCAACTGGTATCGGAGCGCGCTGTATAACGTGCCTATAGCCACTTTTAAGTTATTCAGAAAAACGGTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTg                                                                                                                                                     <  1:253299/251‑1 (MQ=255)                                                               aGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCACTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTg                                                                                                                                                                   >  1:22539/1‑227 (MQ=255)                                                                                              naaTGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCACTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGa                                                                                                                                                    >  1:305586/2‑211 (MQ=255)                                                                                                                                         ggcggATAATTTTATTTTTATTGTCAGCACTGTCATTTATTGGGTTTTTTTGCATCATTAACGATCAACTGGTATCGGAGCGCGCTGTAAAACGTGCCGATAGCCACTTTGATCTTATCCAGAAAATGGGGGGCTGTTGCTTGAAAGTTCGTGATCGTGCGGCCCTGGCAGTAAATGACTCTCTGTATTTATTATAAAATACAGAGGAGATTCAACGCGCCGTGATTCTTAAAATg                                                                                <  1:478770/232‑1 (MQ=255)                                                                                                                                                           nnnnnncannnNNGTCNTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCACTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGa                                                                                                                                                    <  2:305586/144‑1 (MQ=255)                                                                                                                                                                                                  ttAACTNNCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCACTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTg                                                                                                                                                                   <  2:22539/96‑1 (MQ=255)                                                                                                                                                                                                     aCTAGCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCACTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTCACTGtattt                                                                                                                              <  1:23866/130‑1 (MQ=255)                                                                                                                                                                                                        aTCAACTGGTATCGGAGCGCGCGGTAAAACGTACCGATAGCCACTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTCACTGTATTTATTAAAAAATACAGAGGAGATTCAACGCGCCGTGATTCTTAAAATGGAAATGATGCCATTTTTAGACTCGGTGGGACTGGTACTTGATGATAATAAATATTATCTTTTTTCGCGGAGGGCGAAt  >  1:20871/1‑251 (MQ=255)                                                                                                                                                                                                                                      gTGCCGATAGCCACTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAg                                                                                                                                            >  1:162371/1‑83 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                 TCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTCACTGTATTTATTAAAAAATACAGAGGAGATTCAACGCGCCGTGATTCTTAAAATGGAAATGATGCCATTTTTAGACTCGGTGGGACTGGTACTTGATGATAATAAATATTATCTTTTTTCGCGGAGGGCGAAT  >  NC_000913/872874‑873323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑