Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,976,185 G→A S347N (AGC→AAC)  wecE → dTDP‑4‑dehydro‑6‑deoxy‑D‑glucose transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,976,1850GA100.0% 57.0 / NA 21S347N (AGC→AAC) wecEdTDP‑4‑dehydro‑6‑deoxy‑D‑glucose transaminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/15);  total (6/15)

GGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCG  >  NC_000913/3975958‑3976397
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                    
ggCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAACGAGCGCCTGCTGCGCCTGCCGc                                                                                                                                                                                               <  1:334148/251‑1 (MQ=255)
   naaaGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTTCATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCCCTACACCACCAAAGAGAACGAGCGCCTGCTGCCCCTGCCGCTgg                                                                                                                                                                                            >  1:315976/2‑250 (MQ=255)
                                                       gcgcATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGGGAAGATCGCTACACCACCAAAGAGAACGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTTTTGa                                                                                                                                         >  1:277214/1‑250 (MQ=255)
                                                                     cATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGTAAATCATGGCGGTGTTGCATTACATTCCGCTGCACGGGTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCGCCAAAGAGAACGAGCGCCTGCTGCGCCTGCCGCTGTTCt                                                                                                                                                                                         <  1:263940/188‑1 (MQ=255)
                                                                                                      gTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGaacgagcg                                                                                                                                                                                                                <  1:35857/132‑1 (MQ=255)
                                                                                                      gTTGANNAANNNTCNNAAAGAAGCGGAAATCATGGCGGTNGTTTATTACATACCGCCGCTAGGATGGCCNGNCNGGGGNNACTNTTGGGTGTTTNACGGGGGAGATCGCTACACCACCAAAGAGAAAGAGCGCCGGCTGCGCCNGNCGCCCTTCTACAACCTGTCGCCCGTCAATCANCGGACGGGAATTGCGACTTTGTTGNactcnnttntn                                                                                                                              >  2:366570/1‑206 (MQ=255)
                                                                                                             aaCTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAACGAGCTCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTg                                                                                                                                                     <  1:431534/184‑1 (MQ=255)
                                                                                                                    nnaanNNNNCGGNAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAACGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAAc                                                                                                                                                                                    <  2:315976/144‑1 (MQ=255)
                                                                                                                                                 cATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAACGAGCGCCTTCTGCGCCTGCCTCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACTCTGGTCAAGATTGGTGCCGTGTTACTGGTCGGTa                                              <  1:431459/251‑1 (MQ=255)
                                                                                                                                                     aCATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAACGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTg                                                                             <  1:82035/216‑1 (MQ=255)
                                                                                                                                                          ccGCTGCACTGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAACGAGCGCCTGCTGCGCCGGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTATTGTGCCTGATATGTCGTTGGCAAAATCGTCCTTGTGGACGGCGGGTAGTACACTGTTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTgg                                     <  1:366559/251‑1 (MQ=255)
                                                                                                                                                          ccGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAACGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTgg                                     <  1:366570/251‑1 (MQ=255)
                                                                                                                                                              ggCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTGCCACGGTGAATATCGCTACCCCACCAAAGAGAACGAGCGCCTGCTGCGCCTTCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTTCGGTAATTGCGACTttgttg                                                                                                                                          <  1:222933/145‑1 (MQ=255)
                                                                                                                                                                       gCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAACGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTg                         <  1:156436/250‑1 (MQ=255)
                                                                                                                                                                        nccTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAACGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGc                                                                                                               >  1:307055/2‑163 (MQ=255)
                                                                                                                                                                         ncTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAACGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTgg                                                                                                                >  1:96651/2‑161 (MQ=255)
                                                                                                                                                                                         nnggNNNNNTCCNCGGTGAAGATCGCTACACCACCAAAGAGAACGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGc                                                                                                               <  2:307055/144‑1 (MQ=255)
                                                                                                                                                                                            ttGAGTTCCACGGTGAAGATCGCTACACCACCAAAGATAACTAGCGCCTTCTGCGCCTGCCTCTGTTCTACAACCTGGCGCCCTTCAATCAGCTGGCGTTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAg                                                                                                          <  1:225071/147‑1 (MQ=255)
                                                                                                                                                                                              gAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAACGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTggcg  <  1:82902/250‑1 (MQ=255)
                                                                                                                                                                                                gTTCCACGGTGAAGATCGCTACACCACCAAAGAGAACGAGCGCCTGCTGCGCCTGCCGCTGTTCTACANCCTNTCGCNCGNCAATCAGCGTACGGTNATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAANNGNNNCNTTGTGGAc                                                                                             <  2:350166/157‑1 (MQ=255)
                                                                                                                                                                                                gTTCCACGGTGAAGATCGCTACACCACCAAAGAGAACGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGAc                                                                                             >  1:350166/1‑157 (MQ=255)
                                                                                                                                                                                                                         accaAAGAGAACGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACGTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTgg                                                                                               <  1:217770/130‑1 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                    
GGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCG  >  NC_000913/3975958‑3976397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑