Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,088	G→A	G233D (GGC→GAC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,088	0	G	A	100.0%	27.1 / NA	13	G233D (GGC→GAC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/5);  total (8/5)

CTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTG  >  NC_000913/22864‑23299                                                                                                                                                                                                                                 |                                                                                                                                                                                                                    cTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCCACGGTCAACTGCACAAAGGCGAGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGAGGAAGCGGAAGTTGAGTATTACGACAACACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAACTTTGCCGTAAGCAACGTTAACGAccc                                                                                                                                                                                                                  >  1:267514/1‑228 (MQ=255)                      catcCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGCCGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGACCCAATCTAGCTGGTAATCTGGACCACCACGCCGTGGACTctgc                                                                                                                                                                         >  1:262598/1‑248 (MQ=255)                       atcCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGACCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTctgcctgc                                                                                                                                                                     <  1:283736/251‑1 (MQ=255)                                  gggCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGAGGAAGCGGAAGTTGAGTATTACGACAAAACTTATCCGGCCTTCGACGTTGCTTTACAGGCAGTCAATTAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGACCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCa                                                                                                                                                           >  1:489420/1‑250 (MQ=255)                                             tcGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGCGTATTACGACAAAAATTCTCAGTCCATAGACGTTGCTTTCCAGGCAGTCGCTCAGGATGCCCTGAAAGCAACATTTGCCGTAAGCAACGTTAACGACCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGAACTCTCTATTGc                                                                                                                                                >  1:277333/1‑250 (MQ=255)                                                                   aGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACCAAACTTCTCCGTCCATCGAAGTTGCTTTCCAGGCCGTCGCTCAGGATGCAATGAAAGCAAAATTTGCCGTAAGCTACGTTAACGACCCAATCTCGCt                                                                                                                                                                                                         >  1:495719/1‑171 (MQ=255)                                                                                                                               tGAGTATTACGACAAAACTTCTCCGGCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGACCCAATCTCGCTGGTAATCTggn                                                                                                                                                                                              <  1:52463/122‑2 (MQ=255)                                                                                                                                gAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGCTCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGACCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCg                                                                                                                                     >  1:468217/1‑178 (MQ=255)                                                                                                                                gAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGACCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTgg                                                                                                                         <  1:200777/190‑1 (MQ=255)                                                                                                                                                     ccGTCCATCGACGTTGCTTTCCAGGCAGTCGCTCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGACCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTacac                                       >  1:169361/1‑251 (MQ=255)                                                                                                                                                                                          aTGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGACCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAgctg  <  1:139823/251‑1 (MQ=255)                                                                                                                                                                                             naCTGAAAGCAAAATTTGCCGTACGCAACGTTAACGAACCAATCTCGCTGGTCATCTGGACAACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTCTGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTgg                                                                                           >  1:56627/2‑159 (MQ=255)                                                                                                                                                                                                                     gCAACGTTAACGACCCAATCTCGCTGGTAATCTGGACCACCTCGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGAGTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCg              <  1:324654/212‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                    CTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTG  >  NC_000913/22864‑23299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑