Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,400,978 C→T G266S (GGT→AGT)  abgT ← p‑aminobenzoyl glutamate:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,400,9780CT95.0% 52.6 / NA 20G266S (GGT→AGT) abgTp‑aminobenzoyl glutamate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (10/9);  minor base G (0/1);  total (10/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACCTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGACGTGCATTTGCGGATTGAAC  >  NC_000913/1400769‑1401171
                                                                                                                                                                                                                 |                                                                                                                                                                                                 
tAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATTGCATCACGGTGTGATTAATCGTATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTc                                                                                                                                                           <  1:86494/250‑1 (MQ=255)
      cGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGAAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCGGCAGtt                                                                                                                                                    >  1:360702/1‑251 (MQ=255)
        gTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGCCAACAACGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGc                                                                                                                                                                             >  1:274349/1‑224 (MQ=255)
                  cTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGt                                                                                                                                        >  1:211148/1‑251 (MQ=255)
                                    ggTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTa                                                                                                                                                                                        >  1:296824/1‑185 (MQ=255)
                                              ccATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTcc                                                                                                                                    <  1:76087/227‑1 (MQ=255)
                                                                 aaaaGAAAAAAAGAATGATCAGTGACACGATTCCTTTAATAAAGGGTGAGGGCATCACGGTGTGATTAATCGGAGCGCGCAATATCCCGTTTTGCGGGATCGCCATCAGCGCAATCGCAGCAAGAAAAAGTAGCGATACGACACTTGCGATGCGTAAACCAAAACGCTTACTTTCTCGCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTAtn                                                                                         <  1:105657/250‑2 (MQ=255)
                                                                  aaaGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCTCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGGCAATGTCGGCAGTTTCTCATCGCTGTTTCCCTGCCAGTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCa                                                                                        <  1:252391/251‑1 (MQ=255)
                                                                            aGAATGATCAGTTGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAAGCGTATCGCGCAATAGCCCTTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAAc                                                                                                                                                                                     <  1:452880/148‑1 (MQ=255)
                                                                                        ggCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAg                                                                     >  1:175435/1‑248 (MQ=255)
                                                                                                                           ggTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTNNTATGCGNna                                                                                                                                                                                       >  2:22878/1‑99 (MQ=255)
                                                                                                                                     aTCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGAc                     <  1:22878/251‑1 (MQ=255)
                                                                                                                                         gATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGt                                                                                             >  1:185399/1‑175 (MQ=255)
                                                                                                                                                 aaTATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACGTGCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGACGTGCATTTGCgg         <  1:237464/251‑1 (MQ=255)
                                                                                                                                                        cGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGAATTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGACGTGCATTTGCGGATTGAAc  >  1:407407/1‑251 (MQ=255)
                                                                                                                                                                  gATCACCATCAGCGCAATCGCAGAAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGc                                                   >  1:223015/1‑192 (MQ=255)
                                                                                                                                                                    tCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACGTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAAGGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGa                      <  1:157723/219‑1 (MQ=255)
                                                                                                                                                                        cATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGGGGCTCGATGACGTTGGCGGTTATCAgg                                                                                      <  1:386662/151‑1 (MQ=255)
                                                                                                                                                                              nnnnntCGNAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATc                                                                                         <  2:312311/137‑1 (MQ=255)
                                                                                                                                                                              ngcAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATc                                                                                         >  1:312311/2‑142 (MQ=255)
                                                                                                                                                                                                                 |                                                                                                                                                                                                 
TAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACCTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGACGTGCATTTGCGGATTGAAC  >  NC_000913/1400769‑1401171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑