Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,746,932 G→A D288D (GAC→GAT)  ffh ← signal recognition particle protein component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,746,9320GA95.7% 51.7 / NA 24D288D (GAC→GAT) ffhsignal recognition particle protein component
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (14/8);  minor base C (1/0);  total (16/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

TCCGGGATCTGCCCCATGCCCGGCAGCTTGCCCATCAGACTAGCCATGCCGCCCATATTTTTCATCTGGCGCAGCTGCTCAAGAAAGTCGTTGAGATCGAAGCCGTCACCTTTTTTCAGCTTGCTGGCTAATTTCTCTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATCGACGCATGGACTT  >  NC_000913/2746711‑2747176
                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    
tCCGGGATCTGCCCCATGCCCGGCAGCTTGCCCATCAGACTAGCCATGCCGCCCATATTTTTCATCTGGCGCAGCTGCTCAAGAAAGTCGTTGAGATCGACGCCGTCACCTTTTTTCAGCTTGCTGGCTAATTTCTCTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGATCCGGATg                                                                                                                                                                                                                                              >  1:483739/1‑230 (MQ=255)
                       cAGCTTGCCCATCAGACTAGCCATGCCGCCCATATTTTTCATCTGGCGCAGCTGCTCAAGCAAGTCGTTGAGATCGAAGCCGGCACCTTTTTTCAGCTTGCTTGCTAATTTCTCTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCAACAGTACGTAGCCCATGCCGAGCATACGCGACGCGATGAGCTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCACCACCGAGGAACt                                                                                                                                                                                                  >  1:343355/1‑251 (MQ=255)
                            ngCCCATCAGACTAGCCATGCCGCCCATATTTTTCATCTGGCGCAGCTGCTCAAGAAAGTCGTTGAGATCGAAGCCGTCACCTTTTTTCAGCTTGCTGGCTAATTTCTCTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGATCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTgttc                                                                                                                                                                                             >  1:311756/2‑248 (MQ=255)
                                          gCCATGCCGCCCATATTTTTCATCTTGCGCAGCTGCTCAAGAAAGTCGTTGAGATCGAAGCCGACACCTTTTTCCAGCTTGCTGGCTATTTTCTCTGCCTGCGCGAGGTCAACTTTGCTTTCGATATCTTCGCTCAGCGCCAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGATCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGCTCGGTTTGCCAGTGAt                                                                                                                                                                               >  1:356623/1‑251 (MQ=255)
                                           ccATGCCGCCCATATTTTTCATCTGGCGCAGCTGCTCAAGAAAGTCGTTGAGATCGAAGCCGTCACATTTTTTCAGCTTGCTCGCTAATTTCTCTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATAAGCGCCGCGATGCGATCCGGATGGAAc                                                                                                                                                                                                                                          >  1:77261/1‑191 (MQ=255)
                                                              cATCTGGCGCCGCTGCTCAAGAAAGTCGTTGAGATCGAAGCCATCACCTTTTTTCAGCTTGCTGGCTAATTTCTCTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCCGCGAAAGTACGTCGCCCATGCCGAGACTACGCGACGCGATGCGATCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCCACACCGAGGAACTTGATCGGTTTGCCAGTGAtg                                                                                                                                                                              >  1:77713/1‑232 (MQ=255)
                                                                                        cGTTGATATCGAAGCCGTCACCTTTTTTCAGCTTGCTGGCTAATTTCTCTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGTCGCGATGCGATCCGGATGGAACTGCTCCAGCGCCTCAGTTTTCGCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCAc                                                                                                                                  <  1:355231/250‑1 (MQ=255)
                                                                                          tgaagaTCGAAGCCTTCACCTTTTTGCAGCTTGCTGGCTAATTTCTCTGCCTTCGCGCGGTCAACTTTGCTTTCGATATCTGCGATCAGCGAAAGTACGTCTCCCATGCCGTGAATACGCGACGCGATGCGATCCGTATGGAACGTCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGAGCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCAc                                                                                                                                  <  1:355254/245‑1 (MQ=255)
                                                                                           tGAGATCGAAGCCGTCACCTTTTTTCAGCTTGCTGGCTAATGTCTCTGCCTGCGCGCGGGCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGATCCGGAtn                                                                                                                                                                                                                                              <  1:104631/139‑2 (MQ=255)
                                                                                                                   tCAGCTTGCTGGCTAATTTCTCTGCCTGCGCGCGGTCAGCTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGATCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGATTGCCAGCGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGCCAACTTTggacaccac                                                                                                                      >  1:476585/1‑228 (MQ=255)
                                                                                                                      gCTTGCTGGCTAATTTCTCTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGCCAGTACGTCGCCCATGCAGAGAATACGCGCCGCGATGCGATCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAAc                                                                                                   >  1:365495/1‑251 (MQ=255)
                                                                                                                             ggCTAATTTCTCTGCCTGCGCGCGGTCCACTTTGCTTTCGTTATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGATCCGGATGGACCGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCAt                                                                                            >  1:152375/1‑251 (MQ=255)
                                                                                                                                           cctgcGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGATCCGGATGGAACg                                                                                                                                                                                                                                         >  1:241432/1‑96 (MQ=255)
                                                                                                                                                   cgGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGATCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGc                                                                      <  1:139124/251‑1 (MQ=255)
                                                                                                                                                              nnttnNNNNATCNTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGATCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATag                                                                                                                                                                    <  2:311756/144‑1 (MQ=255)
                                                                                                                                                                  tCGATATCTTCGATCAGCGCCAGTAAGTCGCCTTTACCGAGCATACGCGACACGAAACGATCCGGATGGAACGGCTCCAGCGCATCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGATTGCCAGAGATGCGACGAAAGGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTCCGCCGGTAAGCGGTAACGCTTCATTAAATGCTTTAGCCGTATTGGCCGCATCCTGACCGGt                                                       >  1:164598/1‑251 (MQ=255)
                                                                                                                                                                   cGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGATCCGGCTGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGAc                                                           >  1:386959/1‑246 (MQ=255)
                                                                                                                                                                    gATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGATCCGGATGGAACGGCTCCAGCGCCTCAGTTNNCTCGCCNnc                                                                                                                                                                                                             >  2:24750/1‑99 (MQ=255)
                                                                                                                                                                           tNGNTCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACNCGATNCNNTCNTNATNGAACg                                                                                                                                                                                                                                         <  2:241432/64‑1 (MQ=255)
                                                                                                                                                                           tCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGATCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCGCGCCAACACCGAGGAACTTTATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGt                                               <  1:376637/250‑1 (MQ=255)
                                                                                                                                                                                                 ccATGCCGAGAATACGCGACGCGATGCGATCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAAcc                        >  1:434981/1‑251 (MQ=255)
                                                                                                                                                                                                     gCCGAGAATACGCGACGCGATGCGATCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCggg                     <  1:24750/250‑1 (MQ=255)
                                                                                                                                                                                                      ncGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGACATGGCGTCAACCACAAACAGGGTTTCAACCGGGtt                   >  1:133040/2‑251 (MQ=255)
                                                                                                                                                                                                              acgcgacgcgATGCGATCCGAATGGAACGGTTCCCGCACCTAAGTTTCCTCGCAAACACCGAGGAAATTGATCGGTTTGTCAGAGATGTGATGATGAGAGAGCTCCGCACCGACGCGGGCATCGCCGTCCTCTTTGGTCAACACTACGCCGGTAATCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGCCATGGCGTCATCCACAAACAGGTTTTCAACCGGGTTAATCGACg           <  1:108575/251‑1 (MQ=255)
                                                                                                                                                                                                                       gnTGCGATCCGAATGGAACGGCTATACAGCCTCACTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGACAGTGATGTGACGAAAAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACAATACGCCGGTAAGCGGAAAAGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTACTGGCGTCAACAACAAACAGGGTTTCAACCGGGTTAATCGACGCATGGACtt  >  1:394003/1‑251 (MQ=255)
                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    
TCCGGGATCTGCCCCATGCCCGGCAGCTTGCCCATCAGACTAGCCATGCCGCCCATATTTTTCATCTGGCGCAGCTGCTCAAGAAAGTCGTTGAGATCGAAGCCGTCACCTTTTTTCAGCTTGCTGGCTAATTTCTCTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATCGACGCATGGACTT  >  NC_000913/2746711‑2747176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑