| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,636,925 | G→C | R77P (CGC→CCC) | creC → | sensory histidine kinase CreC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,636,925 | 0 | G | C | 95.0% | 56.5 / NA | 21 | R77P (CGC→CCC) | creC | sensory histidine kinase CreC |
| Reads supporting (aligned to +/- strand): ref base G (1/0); major base C (12/7); minor base A (1/0); total (14/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACC > NC_000913/4636722‑4637153 | gggCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAat > 1:2425/1‑251 (MQ=255) ccATTTTAGTCACAGAAGTTCAACCGGGCGTGAGCAGAGAAACGGAGGGGACGTTGATCGACAACGCAACGTTGCTGGTGCAGCTGACGCGCCCCGATTTGATCTCTCGGGACCCAACGCACGGGCAACTGGAGACGGCGTTTAATCAGCTACAACATCCCCAGTTTCGCGCAAATATCCGTGGCATTAAACAAGGGCACAATGAATATCACGTCTATATGAACGATGCGCCGGGCACAGTATTGTTCGa > 1:420064/1‑250 (MQ=255) tAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTGGAGCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGGAACTGGCGCAGGCGTTTAATCAGCTACAACATACCCCGGTTCGCGACAATGTCGGCGGCATTAAAAAAGTGCGCAATGAATATCATGGCTATATGACCGAGGCGCCGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTg > 1:221044/1‑251 (MQ=255) ggCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCCACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACCACATCCCCCGTTTCGCGCCAATATCGGTgg > 1:198740/1‑158 (MQ=255) cGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCa > 1:391902/1‑154 (MQ=255) cGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAACGTATTGTTCGATTCGGCAAATAAAGCCGTTGGAc > 1:177543/1‑241 (MQ=255) aaCGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGCGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGAGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGc > 1:265417/1‑216 (MQ=255) acCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTcgc < 1:81817/108‑1 (MQ=255) ggCGCGTCCCGAGTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTACGATTCGGCAAATAAAGCCGTTGGACAGGAATATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCgcag < 1:73573/249‑1 (MQ=255) ngTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCAGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGCATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGtt > 1:45138/2‑251 (MQ=255) ctctGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTCCAACATCCCCCGTTTCGCGCAAATCTCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGAACGATGCGCAGGGCAACGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGcc > 1:320601/1‑250 (MQ=255) ctctGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCtn < 1:62050/248‑2 (MQ=255) ggggACCCNANNNANNGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTNGCGCCAATATCGGTGNCANTAACNAAGNGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAACGCCGTTGGACAGGATTATTCGCGCTGGCATGACGTCTGNCTANCGttt > 2:356830/1‑203 (MQ=255) ggggACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAATTGCGCAATGAATATCATGTCTATATTACCGATGCGCAGGGCAAAGGATTGTTCTATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTg < 1:356830/204‑1 (MQ=255) nccAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGAGccc > 1:59046/2‑61 (MQ=255) cccAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGaa > 1:140303/1‑250 (MQ=255) ngNATNGGCAANTGGCNCAGGCGTTTAATCAGNTACAACATCCCCCGTTTCGNNCNAGTATNGGTgg < 2:198740/66‑1 (MQ=255) cATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGcc > 1:136347/1‑234 (MQ=255) tCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGTAATGACGTCTGGCTATCGTTGCGTGGTCAGTATGGTGCGCGCAGCACGtt < 1:189215/195‑1 (MQ=255) tCAGCTACAACATCCCCCGTTTCGCGCAAATATCGGGGGCATTAACAAAGTGCGCAGTGAATATCATGTCTATATGACCCATGCGCAGTGCAAAGTATTGTTCGATTCGGc < 1:424926/111‑1 (MQ=255) tCAGCNACAACNTNNGCCGTTTAGCGCCAATATCNGTGGCATTAACAAAGNGCGCNATGAANATNa > 2:189215/1‑66 (MQ=255) gCTACAACATCACCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCAcc > 1:425910/1‑240 (MQ=255) | GGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACC > NC_000913/4636722‑4637153 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |