Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,602,942	G→A	G375G (GGG→GGA)	hyfB →	hydrogenase 4 component B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,602,942	0	G	A	100.0%	41.1 / NA	16	G375G (GGG→GGA)	hyfB	hydrogenase 4 component B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/7);  total (9/7)

CCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGGGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGT  >  NC_000913/2602731‑2603173                                                                                                                                                                                                                    |                                                                                                                                                                                                                                        ccTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATTGGAGCACTAGCGAAACGGATGCCGTTGACAGCCGCAGCATgn                                                                                                                                                                                                  <  1:148899/251‑2 (MQ=255)                 aaaCGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGCCTGTCACTGCACGCCCCGCTGCTCACCGTGGTTGCACTGCTCGGCGCACTGTTTCATCCGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCCATTATTTCGCCGTTCCATACCCACGACATGGAAAAAATGGGAGTACTAGCGAAACGGATGCCGTGGAc                                                                                                                                                                                                               <  1:234672/222‑1 (MQ=255)                                              gtgtGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATGGGAGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCAt                                                                                                                                                                     >  1:164771/1‑235 (MQ=255)                                                                         aCNNNNNGCTNCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATGGGAGCAc                                                                                                                                                                                                                                     <  2:298790/144‑1 (MQ=255)                                                                            ccccGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATGGGAGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCAGGCCTGATGGGGTGCCTCGCGATAGCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCg                                                                                                                       <  1:253719/250‑1 (MQ=255)                                                                                    ctCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGAGCGGGCGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATGGGAGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCCTTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTtctc                                                                                                               >  1:440377/1‑251 (MQ=255)                                                                                             gTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACCTGGAAAAAATGGGAGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCg                                                                                                      >  1:233342/1‑251 (MQ=255)                                                                                                           ngcACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGAAATGGAAAAAATGGGAGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGa                                                                                                                                                                               >  1:90908/2‑164 (MQ=255)                                                                                                                              aaCCATGCTCTGTTCAAAGGGCTTCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATGGGAGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCTCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGGGTcc                                                                     <  1:435557/251‑1 (MQ=255)                                                                                                                                 cATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCCCGAAATGGAAAAAATGGGAGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGc                                                                                                                                                                        >  1:276960/1‑149 (MQ=255)                                                                                                                                                                      gAGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATGGGAGCACGAGCGAAACGGATGCCTTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATc                                                                                                                                                                           <  1:254061/109‑1 (MQ=255)                                                                                                                                                                                    ngTTTGCATACCCACGACATGGAAAAAATGGGAGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCggg                                                                        >  1:146336/2‑194 (MQ=255)                                                                                                                                                                                    cgTTTGCATACCCACGACATGGAAAAAATGGGAGCACTAGCGAATCGGATGCCGTGGACAGCCGCATCATGCCTTATTGGTTGCCTCGTGATATCAGCCATACCTCCGCTGAATGGGTTTATCAGCGAATGGTACCCCTGGCAGTCGCTGTTCTCCCTACGTCGACTGGAAGCCGTAGCGCTACAACTTGCggg                                                                        <  1:167873/194‑1 (MQ=255)                                                                                                                                                                                              cccACGNCNTNGNCAAAATGGGAGCACTANCGAAACGGATGCCGTg                                                                                                                                                                                                                  >  2:481587/1‑46 (MQ=255)                                                                                                                                                                                                            naaaTGGGAGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGt                                                                                          >  1:304322/2‑152 (MQ=255)                                                                                                                                                                                                              nnnnnnggNNNNNGCGNAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGt                                                                                          <  2:304322/144‑1 (MQ=255)                                                                                                                                                                                                              aaTGGGAGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGAATGCCTGCTTGGATGCCTCGCGATATCAACCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCCGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTCCAACTTGCGGGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGt  >  1:257684/1‑238 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                                                                                                        CCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGGGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGT  >  NC_000913/2602731‑2603173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑