Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,600,716	G→A	R80H (CGT→CAT)	bcp →	thiol peroxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,600,716	0	G	A	100.0%	59.0 / NA	22	R80H (CGT→CAT)	bcp	thiol peroxidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/11);  total (11/11)

ATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAAGAGCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAACAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTG  >  NC_000913/2600502‑2600947                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        atCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCTTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCATTTTGCGGAAAAAGAGCTGCTTAACTTTACGCTcc                                                                                                                                                                                                      <  1:377024/250‑1 (MQ=255)                        ggATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCATTTTGCGGAAAAAGAGCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGgtgt                                                                                                                                                                              >  1:176762/1‑251 (MQ=255)                         gATCAAGACGGAGAACAAGTTAATTCGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTCACCGAACGACATGACCCACGGCTGTACCGTACAGGCATGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTCTCAGCACCGATAAACCCGACAAACTCTACCATTTTGAGGAAAAAGAGCTGCTTAACTTTACGCTACTGTCTGATGTGGACCACCAGGtgtg                                                                                                                                                                             >  1:344479/1‑251 (MQ=255)                                  ggAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAACGCAATGACCCGCGGCTGTACCGTACAGGACTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGGATCAGCACCGATAAACCCGAAAAACTCTCCCATTTTGCGGAAAAagag                                                                                                                                                                                                                         >  1:199934/1‑198 (MQ=255)                                                             cAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCATTTTCCGGAAAAAGAGCTGCTTAACTTTACGCTCCTGTCTGATGAg                                                                                                                                                                                           >  1:385886/1‑201 (MQ=255)                                                                      cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGTCCCCCGGGTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTAATGTGCTGGGTATCAGCACCGATAAATCCGAAAAACTCTCCCATTTTGCGGAAAAAGAGCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAACAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAAc                                                                                                                                <  1:507973/251‑1 (MQ=255)                                                                       gttttCTGGTTTATTTCTTCCCGAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGGGTTGAAAAAAGCGGGCATTTATGTGCTGGGTATCAGCACCGATAAACCCGAAAACCTCTCCCATTTTGCGGAAAAATAGCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGATGTGCGAACAATTCGGCGTCTGGGGTGAAAAGTCCTGCATGGGCAAAAcc                                                                                                                               <  1:73386/248‑1 (MQ=255)                                                                                                 gCCATGACCCCCGGCTGTACCGTCCAGGCCTACGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCATTTTGCGGAGAAAGAGCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAACAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCtt                                                                                                     >  1:181374/1‑251 (MQ=255)                                                                                                        cccccGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCATTGTGCGGAAAA‑GAGCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAACAATTCGGCGTCTGGGGTGAAAAGTCCTTCATggn                                                                                                                                      <  1:46383/210‑2 (MQ=255)                                                                                                             ggCTGTACCTTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCATTTTGCGGa                                                                                                                                                                                                                                <  1:425713/116‑1 (MQ=255)                                                                                                                         cagtcctacagCTTACGCGAAAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTTCTTGGTATCAGCACCGATAAACCCGAAAAACGCTCCCATTTTGCGGAAAAAGAGCTGCTGAACTTTACGCTCCTGTCTGATGAAGTCTACCATGTGTGCTAACAATTCTGCTTCGGTTGTTAAAAGTACTTCATAAGCAAAACCTACGATGGCATTCATCTCCGCATCTTTCTTATTGACGCTGATGGCAAAAt                                                                             <  1:430261/241‑1 (MQ=255)                                                                                                                         cAGGCCTGCGGCTTACGCGATAACATGTATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCATGTTGCGGAAAAAGAGCTGCTTAACTTTACGCTCCTGGCTGATGAGGACCTCCAGGTGTGCGAACAATTCGGCTTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGc                                                                                                       <  1:340542/225‑1 (MQ=255)                                                                                                                           ngCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCATTTTGCGGAAAAAGAGCTGCTTAACTTTACGCTCCTGTCtga                                                                                                                                                                                               >  1:121900/2‑135 (MQ=255)                                                                                                                                                cATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCATTTTGCGGAAAAag                                                                                                                                                                                                                           >  1:290490/1‑86 (MQ=255)                                                                                                                                                              aaaaaGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCATTTTGCGGAAAAAGAGCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAACAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTAc                                                                                                                            <  1:423145/167‑1 (MQ=255)                                                                                                                                                                   gCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCATTTTGCGGAAAAAAAGCTGCTTCAATTTAAACTCCTGTATGCTGACGGCAACCCGGTGTGCGAACAATTCGGCGTCTGGGGAGCACAGTCCTTCATGGGCAAAACCTCCGATGGCATTCATCGCATcc                                                                                                         >  1:347071/1‑180 (MQ=255)                                                                                                                                                                   gCGGGCGTTGATGTGCGGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCATTTTGCGGAAAAAGAGCTGCTTAACTTTACGCTCCTGTCNGATNAGGANCANCAGGTGTGCGAACAANTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATNNCNNNCNTCGCATCa                                                                                                         <  2:347071/181‑1 (MQ=255)                                                                                                                                                                      gnCGNTGATGNGCTGNGTATCATCACCGATANACCCGAAAAACTCTCCCATNNTNCGGAANAagag                                                                                                                                                                                                                         <  2:199934/66‑1 (MQ=255)                                                                                                                                                                           tGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCATTTTGCGGAAAAAGCGCTGCTTAACTTTACGATCCTGTCTGATGAGGACCACCAGGTGTGCGAACACTTCGGCGTCTGGGGTGAAACGTCCTTCATGGGCAAATCCTACGATGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAAACAGCAATCACCACGACGTTGTGc                           >  1:503242/1‑251 (MQ=255)                                                                                                                                                                                    gggTATCAGCACCGATAAACCCGAAAAACTCTCCCATTTTGCGGAAAAAGAGCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAACAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGc                  >  1:391284/1‑251 (MQ=255)                                                                                                                                                                                          cAGCACCGATAAACCCGAAAAACTCTCCCATTTTGCGGAAAAAGAGCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGGGCGAACAATTCGGCGGCTGGGGTGAAAAGTCCTTCa                                                                                                                                          <  1:166770/125‑1 (MQ=255)                                                                                                                                                                                                    naaCCCGAAAAACTCTCCCATTTTGCGGAAAAAGAGCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAACAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGCTTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTg  >  1:132186/2‑251 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        ATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAAGAGCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAACAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTG  >  NC_000913/2600502‑2600947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑