Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,777,984 Δ1 bp intergenic (+202/+162) ypjF → / ← ypjA toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter YpjA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,777,9840C.95.8% 89.7 / ‑3.5 24intergenic (+202/+162)ypjF/ypjAtoxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter YpjA
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base . (14/9);  total (15/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACC  >  NC_000913/2777754‑2778196
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                    
ccGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGACGAGACTCCCGCTGTCACATCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTTAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTAAGACTCCGCCAGC‑‑AC‑AATCATGATTGGACGGTGTAAgg                                                                                                                                                                                               >  1:366254/1‑251 (MQ=255)
        ctacaaaCTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCAGCTGTAACCTCCCCTCCCCCGAAAAAATCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATAAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGC‑‑AC‑AATCATGATTGGACGGTGAAAGGACAACAcc                                                                                                                                                                                       >  1:7441/5‑251 (MQ=255)
                           aaaCGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCGCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCTGACATTGTCTGTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTGAACAACAAGTTACACAACCAATTCAGACGCCGCCAGC‑‑AC‑TATCATGATTTGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAg                                                                                                                                                                    <  1:263331/251‑1 (MQ=255)
                                cTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGC‑‑AC‑AATCATGATTGGACGGTGTAAng                                                                                                                                                                                               <  1:392493/219‑1 (MQ=255)
                                                                              gACGAAGAGACTCCCGCTGTAACCTCCC‑TCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACAAAACTGACAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGC‑‑‑CCAATCATGATTGGACGGTGTAAGGaca                                                                                                                                                                                            >  1:363394/1‑175 (MQ=255)
                                                                                                                                        aGGACNNACNNNGGNNCCAAAATGAAAATTGAACTGAAGNTTACAATCAATTTAACAACAAGTTACACANCNANTTCANNCTCNGCCAGC‑‑AC‑AANCATGATTGGACGGTGTAAGGACAACACCAACAAAAAAAGGAAGTTAGANGNCTCAGCAAAACACCGACCAGACGGTGAGGAGNAATAAAAGGATACGCAAAGGAGCcncagannggnannnnnnn                                                                                      >  2:378105/1‑204 (MQ=255)
                                                                                                                                        aGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGC‑‑AC‑AATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGc                                                                                                         <  1:410964/201‑1 (MQ=255)
                                                                                                                                        aGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAATTACACAACCAATTCAGACTCCGCCAGC‑‑AC‑AATCATGATTGGACGGTGTAAGGACAACGCCAACAAAAACAGGAAGTTAGAAGGCTCAGCAAAACACCGACCAAACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCcgc                                                                                                      <  1:378105/204‑1 (MQ=255)
                                                                                                                                          gACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGC‑‑AC‑AATCATGATTGCACGGTGTAAGGACAACACCAACAAACACAGGAAGTTAGAAGTCTCGGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCAGCGGCTcc                                                                                                >  1:447160/1‑208 (MQ=255)
                                                                                                                                                      naCCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGC‑‑AC‑AATCATGATTGGACGGTGTAAGGacaaca                                                                                                                                                                                         >  1:58477/2‑107 (MQ=255)
                                                                                                                                                        ccAAAATGAAAATTGAACTGAAGATTACAATCACTTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGC‑‑AC‑AATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGt                                       >  1:208099/1‑251 (MQ=255)
                                                                                                                                                        ccAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGC‑‑AC‑AATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAgn                                                                                                                                                          <  1:404821/136‑2 (MQ=255)
                                                                                                                                                               naaaaTTGAACTGAAGATTACAATCAATTTAACAACAAGTTAAACAACCCATTCAGACTCCGCCAGC‑‑AC‑AATCATGATTGGCCGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGc                                                                                                                                                          >  1:98053/2‑129 (MQ=255)
                                                                                                                                                                      gAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCACTTCAGACTCCGCCAGC‑‑AC‑AATCATGATTGGACGGTGTAAGGACAACACCAACAAACACAGGAAGTTAGAAGTCTCAGCAAACCACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGa                                                                          >  1:385643/1‑202 (MQ=255)
                                                                                                                                                                           gAAGATTACACTCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGC‑‑AC‑AATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCa                                                                                                                                                            >  1:139940/1‑115 (MQ=255)
                                                                                                                                                                                   caatcaatTTAACAACAAGTTACACAACCAATCCAGACTCCGCCAGC‑‑AC‑AATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACgggg            >  1:259037/1‑251 (MQ=255)
                                                                                                                                                                                               caacaaGTTACACAACCAATTCAGACTCCGCCAGC‑‑AC‑AATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTggg                                                                    <  1:112366/183‑1 (MQ=255)
                                                                                                                                                                                               caacaaGTTACACAACCAATTCAGACNCCGCCA‑‑NNAC‑NATCATGATTGGACGGTGTANGGNNNANNNCNCCAAANACNNGACGTTAGANGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGNAGCCGCGGCNNCTGGTAACATGAAAGCCCACANATGNngnngntcgngan                                                          >  2:112366/1‑182 (MQ=255)
                                                                                                                                                                                                 acaaGTTACACAACCAATTCAGCCTCCGCCAGC‑‑AC‑AATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTg                                                                                              <  1:113588/155‑1 (MQ=255)
                                                                                                                                                                                                 acaaGTTACACAACCAATTCAGACTCNGCCAGC‑‑‑NNAANCATGATTGGACGGTGTAAGNACNNNANNNANCAAAANAGNNAGTTAGAAGNCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGANCCGCGGCTCNNg                                                                                              >  2:113588/1‑155 (MQ=255)
                                                                                                                                                                                                       tACACAACCAATTCAGACTCCGCCAGC‑‑AC‑AATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACAcc  <  1:119134/241‑1 (MQ=255)
                                                                                                                                                                                                         nacaACCAATTCAGACTCCGCCAGC‑‑AC‑AATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCg                                                                                                                                                 >  1:45711/2‑96 (MQ=255)
                                                                                                                                                                                                         nacaACCAATTCAGACTCCGCCAGC‑‑AC‑AATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTaa                                                                                          >  1:95701/2‑151 (MQ=255)
                                                                                                                                                                                                           caACCAATTCAGACTCCGCCAGC‑‑AC‑AATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGccc                                                                               >  1:408552/1‑160 (MQ=255)
                                                                                                                                                                                                           caACCAATTCAGACTCCGCCAGC‑‑AC‑AATCATGATTGGACGGTGTAAGGACAACACAAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAAc                                              <  1:216106/193‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                    
CCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTAACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACC  >  NC_000913/2777754‑2778196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑