Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,095,445	C→T	R116R (CGC→CGT)	yggS →	PLP homeostasis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,095,445	0	C	T	100.0%	42.9 / NA	17	R116R (CGC→CGT)	yggS	PLP homeostasis protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/9);  total (8/9)

GAAAACTACGTTCAGGAAGGGGTAGATAAAATTCGCCACTTTCAGGAACTGGGCGTAACAGGATTAGAATGGCATTTTATTGGCCCGTTGCAGTCTAATAAAAGCCGCCTGGTGGCAGAGCATTTCGACTGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTTAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCGGTCGCTGAACTACCGCGTTTACGTCTGCGCGGGTTGATGGCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCC  >  NC_000913/3095263‑3095681                                                                                                                                                                                       |                                                                                                                                                                                                                                             gAAAACTACGTTCAGGAAGGGGTAGATAAAATTCGCCACTTTCAGGAACTGGGCGTAACAGGATTAGAATGGCATTTTATTGGCCCGTTGCAGTCTAATAAA‑GCCGCCTGGTGGCAGAGCATTTCGACTGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGTCCggn                                                                                                                                                                                                                                         <  1:105157/187‑2 (MQ=255)                                                              gATTAGAATGGCATTTTATTGGCCCGTTGCAGTCTAATAAAAGCCGCCTGGTGGCAGAGCATTTCGACTGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGTCCGGCAGAACTTCCCCCTCTTAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCGGTCGCTGAACTACCGCGTTTACGt                                                                                                             >  1:286088/1‑251 (MQ=255)                                                                                          gCAGTCTAATAAAAGCCGCCTGGTGGCAGAGCATTTCGAATGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAAGGACCAGCGTCCGGCAGAACTTCCACCTCTTAACGTTCTGATTCAAATTAACATTAGTGATGCAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCGGTCGCTGAACTACCGCGTTTCAGTCTgc                                                                                                         >  1:362606/1‑227 (MQ=255)                                                                                             gTCTAATAAAAGCCGCCTGGTGGCAGAGCATTTCGACTGGTGTNATANCATCNACNGTTTGCGCATCGCTANCCGTCTCAACGACCAGCGTCCGGCAGAACTTCCCCCTCTTAACTNNCNNNTNCAAATTaa                                                                                                                                                                                                     <  2:344981/132‑1 (MQ=255)                                                                                             gTCTAATAAAAGCCGCCTGGTGGCAGAGCATTTCGACTGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGTCCGGAAGAACTTCCCCCTCTTAACGTTCTGATTCAAATTaa                                                                                                                                                                                                     >  1:344981/1‑132 (MQ=255)                                                                                               cTAATAAAAGCCGCCTGGTGGCAGAGCATTTCGACTGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGTCCGGCAGAACTTcccc                                                                                                                                                                                                                              >  1:214144/1‑105 (MQ=255)                                                                                                                                 cTGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGACTCAACGACCAGCGTCCGGCAGCACTTCCCACTCTTAACGTTCTGATTCAAATTAACATTAGTGATGACAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCGGTCGCTGAAc                                                                                                                           >  1:80897/1‑170 (MQ=255)                                                                                                                                     ngnCANACCATNGACCNTTTGCGCATCGCTACNCGTCTCAACGACCAGCGTCNNGNATATCNTcccc                                                                                                                                                                                                                              <  2:214144/66‑1 (MQ=255)                                                                                                                                      gtCATACTATCGACCGTTTGCGCATAGCTACCCGTCTCAACGAACAGCGTACGGCAGAACTTCCCCCTCTTAACGTTCTg                                                                                                                                                                                                                <  1:75613/80‑1 (MQ=255)                                                                                                                                                            naTCGCTACCCGTCTCAACGACCAGCGTCCGGCAGAACTTCCCCCTCTTAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCGGTCGCTGAACTACCGCGTTTACGTCTGCGCGGGTTGATGGCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTg                >  1:124555/2‑250 (MQ=255)                                                                                                                                                              tCGCTACCCGTCTCAACGACCAGCGTCCGGCAGAACTTCCCCCTCTTAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAANNCCGGGANNc                                                                                                                                                                     >  2:39660/1‑99 (MQ=255)                                                                                                                                                              tCGCTACCCGTCTCAACGACCAGCGTCCGGCAGAACTTCCCCCTCTTAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCGGTCGCTGAACTACCGCGTTTACg                                                                                                              <  1:39660/154‑1 (MQ=255)                                                                                                                                                                gCTACCCGTCTCAACGACCAGCGTCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAATTAACATTATTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTTAACTGGACGAGCTGGCAGCTGCGGTCGCTGAACTACcgcg                                                                                                                    <  1:340180/146‑1 (MQ=255)                                                                                                                                                                 cTACCCGTCTCAACGACCAGCGTCCGGCAGAACTTCCCCCTCTGAACGTTCTGATTCAAAGTAACATTAGTGATGAAAACAGTAAGTCCGGCATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCGGTCGCTGAACTACCGCGTTTACGTCTGCGCGGGTTGATGGc                                                                                            <  1:257649/169‑1 (MQ=255)                                                                                                                                                                      cGTCTCAACGACCAGCGTCCGGCAGAACTTCCCCCTCTTAACGTTCCGATGCAAATGAGCATTAGTGATGAAAACAGGAATTCCGGGACTCAACTTGCGGAACTGGACTAGCCGGCAGTTGCCGTCGCTGAACTACCGCGTGTACGTCTGCGCGGGTTGATGGCAATCCCTgcg                                                                                  <  1:364335/174‑1 (MQ=255)                                                                                                                                                                         ctcAACGACCAGCGTCCGGCAGAACTTCCCCCTCTTAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCGGTCGCTGAACTACCGCGTTTACGTCTGCGCGGGTTGATGGCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTAccc  >  1:32274/1‑251 (MQ=255)                                                                                                                                                                          tcAACGACCAGCGTCCTGCAGAACTTCCCCCGCTTAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTTGCTGAACTGGACGAGCTGGCAGCTGCGGTCGCGGACCTACCGCGTTGACGTCGGCGCGGGTTGATGGCAACCGCTGCGTCTGAGTCAGAAGAAGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTg                                 <  1:428548/219‑1 (MQ=255)                                                                                                                                                                                       |                                                                                                                                                                                                                                             GAAAACTACGTTCAGGAAGGGGTAGATAAAATTCGCCACTTTCAGGAACTGGGCGTAACAGGATTAGAATGGCATTTTATTGGCCCGTTGCAGTCTAATAAAAGCCGCCTGGTGGCAGAGCATTTCGACTGGTGTCATACCATCGACCGTTTGCGCATCGCTACCCGTCTCAACGACCAGCGCCCGGCAGAACTTCCCCCTCTTAACGTTCTGATTCAAATTAACATTAGTGATGAAAACAGTAAGTCCGGGATTCAACTGGCTGAACTGGACGAGCTGGCAGCTGCGGTCGCTGAACTACCGCGTTTACGTCTGCGCGGGTTGATGGCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCC  >  NC_000913/3095263‑3095681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑