Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	13,942	G→A	V594I (GTT→ATT)	dnaK →	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	13,942	0	G	A	96.7%	80.5 / NA	30	V594I (GTT→ATT)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (21/8);  minor base C (1/0);  total (22/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGA  >  NC_000913/13712‑14181                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                gcgACGCAGAAGCTAACGCCTAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGAGTCGCAACAAGGGCGACCATCTGCTGCACAGAACCCGTAAGCAGGTTGCCGAAGCAGGCGACAAAATGCCGGCTGAAGACAAAACTGCTATCGAGTCTGCGCTGACTGCACTGGAAACTGCTCTGCAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCCCAGATTTCCCAg                                                                                                                                                                                                                                         >  1:365777/1‑239 (MQ=255)            gCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGATTTCCCAGAAACTGATGGAAATCGCCcagc                                                                                                                                                                                                                   >  1:67657/1‑250 (MQ=255)                      nnnnngNCCNNAAGTNTGAAGAGCTGGTACAGACTCGCAANCAGGGCGACCATCTGCTGCACAGCACCCGTANGNAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTANCGAGTCTGCGNTGANNGCACNGNANACTGCTCTGAAAGGGGAAGACAAAGCCGCNATCGAAGCGAAAATGCAGGAACTNNCANNNATNNCCCAg                                                                                                                                                                                                                                         <  2:365777/213‑1 (MQ=255)                           gACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGATTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCcagc                                                                                                                                                                                                    <  1:30534/250‑1 (MQ=255)                                                             aCCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGACGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGATTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAaac                                                                                                                                                                    >  1:263030/1‑248 (MQ=255)                                                                        cATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGATTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCt                                                                                                                                                                          <  1:280322/231‑1 (MQ=255)                                                                                              aGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGATTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAAttt                                                                                                                                >  1:157430/1‑251 (MQ=255)                                                                                               atgggTTTAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGATTTCCCAGAAACTGATGGAAAGCGCCCAGTAGCAACTTGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTg                                                                                                                               <  1:338097/248‑1 (MQ=255)                                                                                                  ggTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGAGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGATTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTga                                                                                                                              >  1:283514/1‑249 (MQ=255)                                                                                                  ggTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACGACTATCGAGGCGGCGCTGACTGCACTGGAAACGGCGATGAAAGGTGAAGACAAAGCCTCTATCGAAGCGAAAATGCAGGAACTGGCACAGATGTCCCAGAAACTGATGGAAAGCGCCcagcan                                                                                                                                                                                                                 <  1:103714/166‑2 (MQ=255)                                                                                                        agaagCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCGCTGACTGCCCTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGACGCGAAAATGCAGGAACTGGCACAGATTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTc                                                                                                                       >  1:255317/1‑250 (MQ=255)                                                                                                                 ngACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGATTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACaaa                                                                                                              >  1:309806/2‑250 (MQ=255)                                                                                                                   aCAAACTGCCGCCTGACGACAACACTGCTATCGAGTATGCGCTGACTGCACTGGCAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGATTTCCCAGAAACTGATGGAAATCGCCCCGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGTTGACGATGTTGTCGACGCTGAATTTGAAGACGTCAAAGACaaaaa                                                                                                            >  1:175988/1‑250 (MQ=255)                                                                                                                    cAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGATTTCCCAGAAACTGATGGAAATCGCCCAGCAGCGACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGa                                                                                                                                    >  1:276708/1‑225 (MQ=255)                                                                                                                                              cTATCGAGTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGATTTCCCCGAACCTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTg                                                                                                                               >  1:266245/1‑204 (MQ=255)                                                                                                                                                  cGAGTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGATTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGACTTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTg                                                                             >  1:176016/1‑250 (MQ=255)                                                                                                                                                             tGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGATTTCCGAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGAtgtt                                                                                                                                               <  1:289993/173‑1 (MQ=255)                                                                                                                                                                    nntGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGATTTCCCAGAAACTGATGGAAAt                                                                                                                                                                                                                           <  2:22365/88‑1 (MQ=255)                                                                                                                                                                    aCTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGATTTCCCAGAAACTGATGGAAAt                                                                                                                                                                                                                           >  1:22365/1‑90 (MQ=255)                                                                                                                                                                       ggAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGCAACTGGCACAGATTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAACGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCACAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAAttt                                                         >  1:466638/1‑249 (MQ=255)                                                                                                                                                                           aCTGCTCTGCACGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGCACCGGCACAGCTTTACCAGCAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGTCTGCTGGTGCTGATGCTTCTGCACACACCCCGCAAGATGACGATGTTGTCGACGCTGAATATGAAGAAGTCACAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCtctc                                                   >  1:292047/1‑251 (MQ=255)                                                                                                                                                                            cTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAAGTGGCACAGATTTACCAGAAACTGATGGAAATCGc                                                                                                                                                                                                                        >  1:381638/1‑85 (MQ=255)                                                                                                                                                                                tctGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGATTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGccc                                              >  1:41540/1‑251 (MQ=255)                                                                                                                                                                                 ctgcaaGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGGACTGGCACAGATTTCCCAGCAACTGCTGGAAATCGCCcagcag                                                                                                                                                                                                                 >  1:267074/5‑87 (MQ=255)                                                                                                                                                                                       ggTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGATTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCtctc                                                   >  1:229981/1‑239 (MQ=255)                                                                                                                                                                                                                  naaaTGCAGGAACTGGCACAGATTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATgg            >  1:128700/2‑251 (MQ=255)                                                                                                                                                                                                                                gggacaGATTTCCCAGAAACTGTTGGTAATCGCCAAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACTGGCGAAGGGGAATTGCCTCGCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAATATGGCTAAGCAAGa  <  1:467579/244‑1 (MQ=255)                                                                                                                                                                                                                                ggCACAGATTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCcagcagca                                                                                                                                                                                                >  1:156578/1‑57 (MQ=255)                                                                                                                                                                                                                                  cacaGATTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAAcgcg                                                                                                                                                              >  1:196601/1‑89 (MQ=255)                                                                                                                                                                                                                                  cacaGATTTCCCAGAAACTGATGGAAATCGCCCAGCACCAACATGCCCAGCCGCAGACTGACGGTGCTGATGCTTATGCAAACAACGCGAACGATGACGCTGTTGTCGACGCTGAATTTGAAGAAGTCCAAGACAAAAAATAATCGCCTTATAAACGGGTAATTCTACTGACACggg                                                                      >  1:503306/1‑177 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                GCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGA  >  NC_000913/13712‑14181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑